2 research outputs found
Microsatellites from sponge genomes: the number necessary for detecting genetic structure in Hemimycale columella populations
10 páginas, 3 figuras, 3 tablasUsing next-generation sequencing technology, we designed a pool of microsatellite
primers and amplified them in 2 physically isolated populations of the Atlanto-Mediterranean
sponge Hemimycale columella, which exhibits particular evolutionary, biological, and ecological
features. The species has contrasting life cycles in shallow and relatively deep waters, and
releases small, predictably low-dispersal larvae. This study experimentally evaluated how many
polymorphic microsatellites would be necessary to detect genetic structure in H. columella populations,
and whether or not the use of compound loci is advisable. By sequencing 1/2 454 GS-FLX
run, we obtained a total of 4208 sequences enclosing microsatellite motifs. We selected 20 microsatellites,
15 of which proved adequate for the genetic study of the sponge populations. The 2 analyzed
populations were genetically structured with all microsatellite combinations assayed, and
the values of the Dest and FST statistics did not increase with increasing number of loci. A weak signal
of genetic structure, however, was shown in bar plots representing membership coefficients
for each individual to each sampling location even with all loci. We conclude that a few polymorphic
loci can detect structure in H. columella populations, but using 12 or more loci notably
enhances the power of the analyses. The study also describes a low-cost protocol for obtaining
microsatellites by the hundreds from non-model, ecologically relevant species, which can be used
to provide information about population isolation, adaptation, and vulnerability.The study was funded by Mar -
SymBiomics project ref. MINECO, I+D+I of Excellence,
CTM2013-43287-P and 2014-SGR120 Benthic Ecology Consolidate
Group from the Generalitat of Catalunya, to M.J.U.Peer reviewe