2 research outputs found
Plasma-activated multi-walled carbon nanotube–polystyrene composite substrates for biosensing
7 páginas, 6 figuras, 2 tablas.-- PACS 87.80.-y Biophysical techniques (research methods)
52.77.Bn Etching and cleaning
85.35.Kt Nanotube devices
07.07.Mp Transducers
79.60.-i Photoemission and photoelectron spectra.Carbon nanotube–polymer composites have shown to be suitable materials for the fabrication of
electrochemical transducers. The exposed surface of these materials is commonly passivated by
a very thin layer of the polymer component that buries the conductive carbon particles. Working
with multi-walled carbon nanotube–polystyrene (MWCNT–PS) composite structures, it was
previously described how a simple low power oxygen plasma process produced an effective
etching of the composite surface, thereby exposing the conductive surface of CNTs. This work
shows how this plasma process not only gave rise to a suitable composite conductive surface for
electrochemical sensing but simultaneously exposed and created a high density of
oxygen-containing functional groups at both the CNT and the PS components, without affecting
the material’s mechanical stability. These chemical groups could be effectively modified for the
stable immobilization of biological receptors. A detailed chemical characterization of the
plasma-activated composite surface was possible using x-ray photoelectron spectroscopy. The
material reactivity towards the tethering of a protein was studied and protein–protein
interactions were then evaluated on the modified composite transducers by scanning electron
microscopy. Finally, an amperometric immunosensor approach for the detection of rabbit
Immunoglobulin G target analyte was described and a minimum concentration of 3 ng ml−1
was easily measured.This work was supported by Consolider-Ingenio 2010 (project
no. CSD2006-00012) and MICINN (project no. TEC2007-
68012-C03-01). CF-S and EM acknowledge the Ministerio
de Educación y Ciencia for the financial support through the
Programa Ramon y Cajal.Peer reviewe
Plasma-activated multi-walled carbon nanotube–polystyrene composite substrates for biosensing
7 páginas, 6 figuras, 2 tablas.-- PACS 87.80.-y Biophysical techniques (research methods)
52.77.Bn Etching and cleaning
85.35.Kt Nanotube devices
07.07.Mp Transducers
79.60.-i Photoemission and photoelectron spectra.Carbon nanotube–polymer composites have shown to be suitable materials for the fabrication of
electrochemical transducers. The exposed surface of these materials is commonly passivated by
a very thin layer of the polymer component that buries the conductive carbon particles. Working
with multi-walled carbon nanotube–polystyrene (MWCNT–PS) composite structures, it was
previously described how a simple low power oxygen plasma process produced an effective
etching of the composite surface, thereby exposing the conductive surface of CNTs. This work
shows how this plasma process not only gave rise to a suitable composite conductive surface for
electrochemical sensing but simultaneously exposed and created a high density of
oxygen-containing functional groups at both the CNT and the PS components, without affecting
the material’s mechanical stability. These chemical groups could be effectively modified for the
stable immobilization of biological receptors. A detailed chemical characterization of the
plasma-activated composite surface was possible using x-ray photoelectron spectroscopy. The
material reactivity towards the tethering of a protein was studied and protein–protein
interactions were then evaluated on the modified composite transducers by scanning electron
microscopy. Finally, an amperometric immunosensor approach for the detection of rabbit
Immunoglobulin G target analyte was described and a minimum concentration of 3 ng ml−1
was easily measured.This work was supported by Consolider-Ingenio 2010 (project
no. CSD2006-00012) and MICINN (project no. TEC2007-
68012-C03-01). CF-S and EM acknowledge the Ministerio
de Educación y Ciencia for the financial support through the
Programa Ramon y Cajal.Peer reviewe