2 research outputs found
Novel Tacrine – 8-Hydroxyquinoline Hybrids as Multifunctional Agents for the Treatment of Alzheimer’s Disease, with Neuroprotective, Cholinergic, Antioxidant, and Copper-Complexing Properties
Tacrine and PBT2 (an 8-hydroxyquinoline derivative) are well-known drugs that inhibit cholinesterases
and decrease β-amyloid (Aβ) levels by complexation of redox-active metals, respectively. In this work,
novel tacrine-8-hydroxyquinoline hybrids have been designed, synthesized, and evaluated as potential
multifunctional drugs for the treatment of Alzheimer’s disease. At nano- and subnanomolar concentrations
they inhibit human acetyl- and butyrylcholinesterase (AChE and BuChE), being more potent
than tacrine. They also displace propidium iodide from the peripheral anionic site of AChE and thus
could be able to inhibit Aβ aggregation promoted by AChE. They show better antioxidant properties
than Trolox, the aromatic portion of vitamin E responsible for radical capture, and display neuroprotective
properties against mitochondrial free radicals. In addition, they selectively complex Cu(II), show
low cell toxicity, and could be able to penetrate the CNS, according to an in vitro blood-brain barrier
modelThe authors gratefully acknowledge the
financial support of SpanishMinistry of Science and Innovation
(Projects SAF2006-01249, SAF2009-13015-C02-01, and
SAF2009-12150), Community ofMadrid (Programa de Actividades
de IþDentre Grupos de Investigación en Biociencias,
Project S-SAL/0275/2006), and the Institute ofHealth Carlos
III (Projects Red RENEVAS, RETICS-RD06/0026). The
fellowships toM.I.F.-B. and G.C.G.-M. from CSIC are also
acknowledged.Peer reviewe