2 research outputs found
Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis
25 Pag., 2 Tabl., 5 Fig. The definitive version is available at: http://www.elsevier.de/jplphIron deficiency induces several mechanisms in response to iron shortage in plants. Metabolic
changes occur to sustain the increased iron uptake capacity of Fe-deficient plants. We evaluated the
metabolic changes of three Prunus rootstocks submitted to iron chlorosis and their different
response for tolerance using measurements of metabolites and enzymatic activities. The more
tolerant rootstocks Adesoto (Prunus insititia) and GF 677 (Prunus amygdalus × Prunus persica),
and the more sensitive Barrier (P. persica × Prunus davidiana) were grown hydroponically in iron
sufficient and deficient conditions during two weeks. Sugar, organic and amino acid concentrations
of root tips were determined after two weeks of iron shortage by proton nuclear magnetic resonance
spectroscopy of extracts. Complementary analyses of organic acids were performed by liquid
chromatography coupled to mass spectrometry. The major soluble sugars found were glucose and
sucrose. The major organic acids were malic and citric acids, and the major amino acid was
asparagine. Iron deficiency increased root sucrose, total organic and amino acid concentrations and
phosphoenolpyruvate carboxylase activity. After two weeks of iron deficiency, the malic, citric and
succinic acid concentrations increased in the three rootstocks, although no significant differences
were found among genotypes with different tolerance to iron chlorosis. The tolerant rootstock
Adesoto showed higher total organic and amino acid concentrations. In contrast, the susceptible
rootstock Barrier showed lower total amino acid concentration and phosphoenolpyruvate
carboxylase activity values. These results suggest that the induction of this enzyme activity under
iron deficiency, as previously shown in herbaceous plants, indicates the tolerance level of
rootstocks to iron chlorosis. The analysis of other metabolic parameters, such as organic and amino
acid concentrations, gives complementary information for selection of genotypes tolerant to iron
chlorosis.This work was partly funded by the Spanish-French bilateral
cooperation programs (Picasso Program-HF2003-273; Aquitaine-
Aragón Aq23 and Aq25) and the Spanish MICINN (Ministerio de
Ciencia e Innovación, grants AGL-2005-05533, AGL-2008-00283
and AGL2009-09018). Sergio Jiménez was supported by an I3P
fellowship from CSIC/FSE (Consejo Superior de Investigaciones Científicas/
Fondo Social Europeo) and a travel fellowship from DGA/CAI
(CA 5/03).Peer reviewe
Metabolic response in roots of Prunus rootstocks submitted to iron chlorosis
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699International audienceIron deficiency induces several responses to iron shortage in plants. Metabolic changes occur to sustain the increased iron uptake capacity of Fe-deficient plants. We evaluated the metabolic changes of three Prunus rootstocks submitted to iron chlorosis and their different responses for tolerance using measurements of metabolites and enzymatic activities. The more tolerant rootstocks Adesoto (Prunus insititia) and GF 677 (Prunus amygdalus × Prunus persica), and the more sensitive Barrier (P. persica × Prunus davidiana) were grown hydroponically in iron-sufficient and -deficient conditions over two weeks. Sugar, organic and amino acid concentrations of root tips were determined after two weeks of iron shortage by proton nuclear magnetic resonance spectroscopy of extracts. Complementary analyses of organic acids were performed by liquid chromatography coupled to mass spectrometry. The major soluble sugars found were glucose and sucrose. The major organic acids were malic and citric acids, and the major amino acid was asparagine. Iron deficiency increased root sucrose, total organic and amino acid concentrations and phosphoenolpyruvate carboxylase activity. After two weeks of iron deficiency, the malic, citric and succinic acid concentrations increased in the three rootstocks, although no significant differences were found among genotypes with different tolerance to iron chlorosis. The tolerant rootstock Adesoto showed higher total organic and amino acid concentrations. In contrast, the susceptible rootstock Barrier showed lower total amino acid concentration and phosphoenolpyruvate carboxylase activity values. These results suggest that the induction of this enzyme activity under iron deficiency, as previously shown in herbaceous plants, indicates the tolerance level of rootstocks to iron chlorosis. The analysis of other metabolic parameters, such as organic and amino acid concentrations, provides complementary information for selection of genotypes tolerant to iron chlorosis