Resistance of casein-derived bioactive peptides to simulated gastrointestinal digestion

The resistance of six casein-derived peptides, including antihypertensive peptides RYLGY, AYFYPEL and YQKFPQY, to simulated gastrointestinal digestion and the effect on angiotensin-converting enzyme (ACE)-inhibitory activity were evaluated. After digestion, peptides RYLGY, AYFYPEL, and YQKFPQY were partly hydrolysed by the digestive enzymes. RYLGY and AYFYPEL maintained potent ACE-inhibitory activity, with IC50 values as low as 9.3 and 4.7μgmL-1, respectively. Digestion fragments were sequenced and then synthesised to evaluate their activity. Several showed potent ACE-inhibitory activity, which could explain the invitro activity of the digests. A notable antioxidant activity was also observed. Since AYFYPEL was less susceptible to digestion, we focused on the antihypertensive activity in spontaneously hypertensive rats of the main digestion fragments of RYLGY. Interestingly, these peptides showed moderate effects invivo. This suggests that the undigested fraction could also contribute to the invivo effects of RYLGY and AYFYPEL, and other minor fragments may also participate. © 2013 Elsevier Ltd.This work has received financial support from the projects AGL2011-24643, Consolider Ingenio 2010 FUN-C-Food CSD2007-063 from Ministerio de Ciencia e Innovación, and project P2009/AGR-1469 from Comunidad de Madrid.Peer Reviewe