1 research outputs found
Colonisatoin of Pinus halepensis roots by Pseudomonas fluorescens and interaction with the ectomycorrhizal fungus Suillus granulatus
8 pages, figures, and tables statistics.Colonisation of Pinus halepensis roots by GFP-tagged Pseudomonas fluorescens Aur6 was monitored by epifluorescence microscopy
and dilution plating. Aur6-GFP was able to colonise and proliferate on P. halepensis roots. Co-inoculation with the ectomycorrhizal
fungus Suillus granulatus did not affect the bacterial colonisation pattern whereas it had an effect on bacterial density.
Bacterial counts increased during the first 20 days of seedling growth, irrespective of seedlings being mycorrhizal or not. After
40 days, bacterial density significantly decreased and bacteria concentrated on the upper two-thirds of the pine root. The presence
of S. granulatus significantly stimulated survival of bacteria in the root elongation zone where fungal colonisation was higher. The
number of mycorrhizas formed by S. granulatus was not affected by co-inoculation with Aur6-GFP. Neither Aur6-GFP nor S. granulatus
stimulated P. halepensis development when inoculated alone, but a synergistic effect was observed on seedling growth when
bacteria and fungus were co-inoculated.Peer reviewe