1 research outputs found
Evaluation of a novel 16S rRNA/tRNAVal mitochondrial marker for the identification and phylogenetic analysis of shrimp species belonging to the superfamily Penaeoidea
8 páginas, 4 figuras, 5 tablasIn this study, we infer the phylogenetic relationships within commercial shrimp using sequence data
from a novel mitochondrial marker consisting of an approximately 530-bp region of the 16S ribosomal
RNA (rRNA)/transfer RNA (tRNA)Val genes compared with two other mitochondrial genes: 16S rRNA
and cytochrome c oxidase I (COI). All three mitochondrial markers were considerably AT rich, exhibiting
values up to 78.2% for the species Penaeus monodon in the 16S rRNA/tRNAVal genes, notably higher than
the average among other Malacostracan mitochondrial genomes. Unlike the 16S rRNA and COI genes, the
16S rRNA/tRNAVal marker evidenced that Parapenaeus is more closely related to Metapenaeus than to Solenocera,
a result that seems to be more in agreement with the taxonomic status of these genera. To our
knowledge, our study using the 16S rRNA/tRNAVal gene as a marker for phylogenetic analysis offers
the first genetic evidence to confirm that Pleoticus muelleri and Solenocera agassizi constitute a separate
group and that they are more related to each other than to genera belonging to the family Penaeidae.
The 16S rRNA/tRNAVal region was also found to contain more variable sites (56%) than the other two
regions studied (33.4% for the 16S rRNA region and 42.7% for the COI region). The presence of more variable
sites in the 16S rRNA/tRNAVal marker allowed the interspecific differentiation of all 19 species
examined. This is especially useful at the commercial level for the identification of a large number of
shrimp species, particularly when the lack of morphological characteristics prevents their differentiation.The authors acknowledge the financial
support from the National Food Program of the INIA (Spanish
Ministry for Education, project CAL-03-030-C2-1) and from the
PGIDIT Research Program in Marine Resources (project PGIDIT04RMA261004PR)
of the Xunta de Galicia (Galician Council for
Industry Commerce and Innovation).Peer reviewe