Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus

Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium. © 2014, Society for Industrial Microbiology and Biotechnology.This study was supported by grants AGL2012-40084-C03-01, AGL2012-35814 and RM2011-00003-00-00 from the Spanish Ministry of Economics and Competitiveness and by European Commission FP7 Initial Training Network (contract 238490).Peer reviewe