1 research outputs found
Evaluation of the antioxidant capacity of lupin sprouts germinated in the presence of selenium.
Antioxidant capacity of 5 DAS (days after
seeding) lupin sprouts germinated in the presence of selenate
or selenite was determined by cyclic voltammetry
(CV), Folin-Ciocalteu reagent (FCR) application and
photochemiluminescence (PCL) methods. The hydrophilic
(80% methanol) and lipophillic (hexane/methanol) extracts
of 5 DAS lupin sprouts germinated in the absence of
selenium (control sprouts) showed higher antioxidant
capacity than ungerminated seeds. Five DAS sprouts produced
in the presence of higher concentration of selenate or
selenite (6–8 mg/L) showed increased total antioxidant
capacity formed by both hydrophilic and lipohillic antioxidants
when evaluated by CV and FCR methods. The
hydrophilic extracts from sprouts produced in the presence
of low concentration of selenate (2–6 mg/L) showed a
significantly higher antioxidant capacity of water-soluble
compounds (PCL ACW) while no changes were noted in
those germinated in the presence of the highest concentration
in relation to the control sprouts. In contrast, the
antioxidant capacity of lipid-soluble compounds (PCL
ACL) lowered significantly in relation to the control
sprouts. The similar changes in antioxidant capacity of
sprouts produced in the presence of selenite were found by
both PCL assays. Comparison of PCL with CV and FCR
reducing capacity assays has shown that these methods
yielded considerably different chemical information.
Moreover, the changes in total antioxidant and total
reducing capacity of sprouts germinated in the presence of
either selenate or selenite provided by CV and FCR assays
were higher than those obtained by photochemiluminesce
measurements. It can be suggested to use 6–8 mg/L of
selenite rather than selenate in order to obtain a lupin
sprouts with high antioxidant capacity.Peer reviewe