1 research outputs found
Molecular identification of cephalopod species by FINS and PCR-RFLP of a cytochrome b gene fragment
6 páginas, 4 figuras, 3 tablasIdentification of commercial species is a relevant
issue to assure the correct labeling of seafood
products. In this work two different molecular techniques,
FINS (Forensically Informative Nucleotide Sequencing)
and PCR-RFLP (Polymerase Chain Reaction-Restriction
Fragment Length Polymorphism) were developed to
identify 8 cephalopod species (families Loliginidae and
Ommastrephidae) employing a fragment of the cytochrome
b gene. DNA amplification for all of the species
was carried out with a new set of specific primers
designed in this study for cephalopods. FINS is a
technique based on DNA sequencing, while PCR-RFLP
allows direct species identification by comparing specific
DNA restriction patterns. Both techniques are useful for
cephalopod species identification. 17 food products
(mainly “squid rings”) were analyzed and the species
employed for their manufacture identified by FINS.The authors are grateful to the European
Commission, Xunta de Galicia and Comisi n Interministerial de
Ciencia y Tecnolog a for their financial support through research
grants FAIR CT97–3061, XUGA 40202 B 98 and ALI1997–1972-
CE.Peer reviewe