Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin

Different profiles were obtained by capillary zone electrophoresis (CZE) of human ery- thropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physio- logical fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.The authors are grateful to European Pharmacopoeia for providing rEPO BRP and to Dr. J. Segura (IMIM, Bar- celona, Spain) for the uEPO samples used in this work. Dr. Segura is also gratefully acknowledged for valuable comments. This work was supported by 1767/2000/ HMS/sls (International Olympic Committee) project.Peer reviewe

Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin

Different profiles were obtained by capillary zone electrophoresis (CZE) of human ery- thropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physio- logical fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.The authors are grateful to European Pharmacopoeia for providing rEPO BRP and to Dr. J. Segura (IMIM, Bar- celona, Spain) for the uEPO samples used in this work. Dr. Segura is also gratefully acknowledged for valuable comments. This work was supported by 1767/2000/ HMS/sls (International Olympic Committee) project.Peer reviewe