1 research outputs found
Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells
Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FADâdependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by dropâcasting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazoniumâmodified NPG electrode. The stability of the bilirubinâoxidaseâmodified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOxâmodified NPG cathode (500â
nm thickness) and a GDHâmodified anode (300â
nm), which generated power densities of 17.5 and 7.0â
ÎŒWâcmâ2 in phosphateâbuffered saline and artificial serum, respectively.This project has received funding from the European Union's Seventh Framework Programme for research, technological development and demonstration under grant agreement no. 607793 (BIOENERGY).Peer reviewe