In vivo selection of resistant E. coli after ingestion of milk with added drug residues.

Antimicrobial resistance represents a major global threat to modern medicine. In vitro studies have shown that very low concentrations of drugs, as frequently identified in the environment, and in foods and water for human and animal consumption, can select for resistant bacteria. However, limited information is currently available on the in vivo impact of ingested drug residues. The objective of our study was to evaluate the effect of feeding preweaned calves milk containing antimicrobial drug residues (below the minimum inhibitory concentration), similar to concentrations detected in milk commonly fed to dairy calves, on selection of resistant fecal E. coli in calves from birth to weaning. At birth, thirty calves were randomly assigned to a controlled feeding trial where: 15 calves were fed raw milk with no drug residues (NR), and 15 calves were fed raw milk with drug residues (DR) by adding ceftiofur, penicillin, ampicillin, and oxytetracycline at final concentrations in the milk of 0.1, 0.005, 0.01, and 0.3 µg/ml, respectively. Fecal samples were rectally collected from each calf once a week starting at birth prior to the first feeding in the trial (pre-treatment) until 6 weeks of age. A significantly greater proportion of E. coli resistant to ampicillin, cefoxitin, ceftiofur, streptomycin and tetracycline was observed in DR calves when compared to NR calves. Additionally, isolates from DR calves had a significant decrease in susceptibility to ceftriaxone and ceftiofur when compared to isolates from NR calves. A greater proportion of E. coli isolates from calves in the DR group were resistant to 3 or more antimicrobial drugs when compared to calves in the ND group. These findings highlight the role that low concentrations of antimicrobial drugs have on the evolution and selection of resistance to multiple antimicrobial drugs in vivo

A new subunit vaccine based on nucleoprotein nanoparticles confers partial clinical and virological protection in calves against bovine respiratory syncytial virus

Human and bovine respiratory syncytial viruses (HRSV and BRSV) are two closely related, worldwide prevalent viruses that are the leading cause of severe airway disease in children and calves, respectively. Efficacy of commercial bovine vaccines needs improvement and no human vaccine is licensed yet. We reported that nasal vaccination with the HRSV nucleoprotein produced as recombinant ringshaped nanoparticles (NSRS) protects mice against a viral challenge with HRSV. The aim of this work was to evaluate this new vaccine that uses a conserved viral antigen, in calves, natural hosts for BRSV. Calves, free of colostral or natural anti-BRSV antibodies, were vaccinated with NSRS either intramuscularly, or both intramuscularly and intranasally using MontanideTM ISA71 and IMS4132 as adjuvants and challenged with BRSV. All vaccinated calves developed anti-N antibodies in blood and nasal secretions and N-specific cellular immunity in local lymph nodes. Clinical monitoring post-challenge demonstrated moderate respiratory pathology with local lung tissue consolidations for the non vaccinated calves that were significantly reduced in the vaccinated calves. Vaccinated calves had lower viral loads than the nonvaccinated control calves. Thus NSRS vaccination in calves provided cross-protective immunity against BRSV infection without adverse inflammatory reaction

Conditioned place preference reveals ongoing pain in calves 3 weeks after disbudding.

Hot-iron disbudding, a routine procedure that prevents horn bud growth through cauterization, is painful for calves. The resulting burns remain sensitive to touch for weeks, but it is unknown whether calves experience ongoing, non-evoked pain. We evaluated conditioned place preference for analgesia in 44 calves disbudded or sham-disbudded 6 hours (Day 0) or 20 days (Day 20) before testing (n = 11/treatment). Calves were conditioned to associate the effects of a lidocaine cornual nerve block with the location and pattern of a visual stimulus, and a control injection of saline with the contrasting stimulus. On Day 0, disbudded calves tended to prefer the lidocaine-paired stimulus over the saline-paired one, suggesting that they found analgesia rewarding. On Day 20, sham calves avoided the lidocaine-paired stimulus, consistent with humans' experience of this drug being painful. Disbudded calves on Day 20 did not show this aversion, suggesting that they traded off the short-term pain of the lidocaine with the longer-term analgesia provided. Day 0 sham calves did not avoid the lidocaine-paired stimulus, likely because they received less than half the dose of Day 20 calves during conditioning. Thus, higher doses of lidocaine are aversive to uninjured animals, but disbudded calves are willing to engage in this cost. We conclude that calves experience ongoing pain 3 weeks after disbudding, raising additional welfare concerns about this procedure

Eating quality of Holstein bull calves fed only grass or purely herbs matches that of concentrate-fed veal calves

Organic meat production from Holstein calves born in dairy herds require that the bull calves are raised outdoor at least 6 months a year, and on large quantities of roughage in the diet. This study aimed at elucidating if Holstein bull calves fed either purely grass or purely herbs prior to slaughter would differ in meat quality traits, fatty acid composition and sensory profile and if they differed in quality aspects from concentrate-fed veal calves

Dairy calves' preference for rearing substrate

Rearing substrate is an important component of the pre-weaning environment of dairy calves. Traditional substrate types, such as sawdust, are becoming difficult and/or expensive for farmers to obtain in New Zealand. Therefore, there is a need to evaluate alternative rearing substrates for dairy calves that that are economically viable for farmers, readily available and provide an acceptable level of animal welfare. The preference of dairy calves for four different rearing substrates and the effects on behaviour and physiology were evaluated. At 1 wk of age, 24 calves were housed in groups of four, in pens which were evenly divided into four rearing substrates: sawdust, rubber, sand and stones. During the first 3 d calves were given free access to all four substrates. Calves were then restricted to each substrate type for 48 h. In order to rank preference, calves were subsequently exposed to two surfaces simultaneously for 48 h until calves experienced all six treatment combinations. Finally, calves were given free access to all four substrates simultaneously for 48 h. Lying behaviour and location in the pen was recorded for 24 h at the end of each experimental period using handycams and accelerometers. Preference was determined based on lying times on each substrate. The insulating properties of each substrate were assessed using iButtons®. During the initial free choice period, the proportion of time spent standing (p < .001) and lying (p < .001) was influenced by substrate. Calves spent a higher proportion of time on sawdust (88%) than all other substrates (rubber: 6%, sand: 4% and stones: 3%). When restricted to each substrate, calves spent more (p .05) of rearing substrate on the frequency of jumps, buck/kicks, head to object and mount/frontal pushing. Calves spent more (p .05) of rearing substrate on the number and duration of lying bouts. We detected no effect (p > .05) of rearing substrate on concentrations of cortisol, lactate, glucose, or white blood cell, neutrophil and lymphocyte count or the neutrophil:lymphocyte ratio. The insulating properties were greatest for sawdust and lowest for sand. During the pairwise choice period, calves had a strong preference for one substrate over another, spending on average, 89% of their time on the preferred surface. Calves preference ranking was for sawdust, rubber, sand then stones as determined by the proportion of time spent on each surface. At the end of the study, when given free access to all rearing substrates again, calves spent a higher proportion of time on sawdust (85%) than all other substrates (rubber: 5%, sand: 7% and stones: 3%). In conclusion, dairy calves showed a clear preference for sawdust over rubber, sand and stones. This preference remained consistent over the course of the study. The calves’ preference for sawdust may be associated with the physical and thermal properties in comparison to the alternative substrates. However, factors such as cost to the farmer, availability and practicality of alternative substrates need to be considered along with animal preferences before any recommendations can be made

Female reproductive strategies and mother-calf relationships of common dolphins (Delphinus delphis) in the Hauraki Gulf, New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Zoology at Massey University, Albany, New Zealand

This study reviewed the habitat use, social organisation and behaviour of common dolphin groups categorised by their youngest member, as well as the behaviour of common dolphin calves of different age classes. Most newborn calves were sighted over late spring and early summer, and in water temperatures warmer than for other groups. The water depths at which common dolphin groups were found did not vary according to the age class of their youngest member. However, only groups containing newborns or infants were sighted under 20 metres of water depth. A high percentage of groups encountered contained calves (82.5%). suggesting the importance of the Hauraki Gulf for common dolphins' reproduction. Groups of common dolphins containing calves were found to be of larger size than any other group type. All groups proved to be similarly involved in different behavioural states and showed comparable patterns of association with other species, although groups including newborns were significantly less likely to be seen associated with whales and birds. Groups of common dolphins as a whole did not show a differential reaction to the boat according to the age class of their youngest member. However, mothers and their young calves kept greater distances to the boat than mothers and older calves did. suggesting that the boat may be perceived as a threat during the newborn period. Most of the behaviours that characterise mother-calf relationships varied according to calf age class. The occurrence, frequency, duration and distance of separations increased with older aged calves. A similar increase was found in the time spent without the mother, in the occurrence of association with non-mother dolphins, in the time spent in 'echelon position', and in both mothers' and calves' dive time. Mothers also had longer dive times than calves. Older aged calves tend to present the lowest proportion of synchronous breaths. These changes are likely to represent a gradual increase in calves' independence. Results from this study have extended our knowledge of common dolphins' reproductive ecology, demonstrated that studies of mother-calf relationships in pelagic species of dolphins can he achieved, and allowed future research needs to he identified and management recommendations to be made

Reducing cross-sucking of group housed calves by an environmental enriched building design

Group housing is a natural and economic way of raising calves. One major disadvan-tage of group housing calves is the occurrence of cross-sucking as an abnormal behaviour. The main objective of our study on organic calf housing was the reduction of cross-sucking related to milk feeding. A new feeding stall with automatic gates was attached to an automatic teat feeder. One gate opened to an enriched area immedi-ately when the calves had finished their milk meal. In this zone calves could use rub-ber teats or a hay net (treatment optimized). The design for the control group used a simple feeding stall without attached enriched zone. 168 female calves (German Holstein) from one origin (herd) were randomly assigned to 14 groups with 12 calves each. Thus, each treatment could be repeated seven times. Two groups were used for preliminary testing of housing and technique. Calves were fed whole milk, according to EU-organic-farming-guidelines. The behaviour of calves was observed directly for 20 minutes following milk intake. In addition, 24 hour video observation completed the behavioural monitoring. The videos were analyzed with the continuous sampling method; statistical analysis was done with the SAS statistical software package and non-parametric procedures. As one result, cross-sucking of calves decreased signifi-cantly in the optimized housing treatment compared to the control treatment: cross-sucking behaviour occurred for 12% of the calves of the optimized treatment vs. 60% cross-sucking calves in the control treatment. Regarding the intensity of cross-sucking, the control group showed 200 cross-sucking bouts per 100 calves and meal, in comparison with a frequency of 16 bouts of the optimized treatment. Our study supports the thesis, that design of housing and environment could be used to reduce cross-sucking of group housed calves. However, future research should be done to evaluate interactions between feeding techniques, environment and physiological processes related to cross-sucking behaviour of calves

PRECONDITIONING BEEF CALVES: ARE EXPECTED PREMIUMS SUFFICIENT TO JUSTIFY THE PRACTICE?

The concept of preconditioning calves has been around for a long time, yet adoption of the practice has been slow. Current trends in the beef industry likely will increase interest in preconditioning programs. This research estimates premiums received for preconditioned calves and the expected returns from a preconditioning program. Preconditioned calves sold in the fall received a premium of approximately $4.50-$5.50/cwt relative to non-preconditioned calves. Premiums were lower for calves sold in the winter, heavier calves, and when cattle markets were strong. Based on a 45-day post-weaning preconditioning program, cow-calf producers can increase returns about $14/head compared to selling calves at weaning.Livestock Production/Industries,

Rearing calves outdoors with and without calf jackets compared with indoor housing on calf health and live-weight performance

peer-reviewedThe objective of this study was to compare the effects of rearing calves outdoors, with and without all-weather calf jackets, with calves reared indoors on calf immunity and animal performance. In February 1999, male Holstein calves (mean (s.e.) weight 55 (1.90) kg) were randomly assigned to one of three treatments (n=30 per treatment): 1) outdoors with jacket, (J; mean age 19 (s.e. 2.0) days); 2) outdoors without jacket (NJ; mean age 19 (s.e. 1.8) days), and 3) indoors on straw (I; mean age 19 (s.e. 1.0) days). Calves received an individual allowance of 25 kg of milk replacer dry matter during the first 42 days with ad libitum access to a concentrate ration from day 0 to 63. The jackets were removed from the calves on day 42. Live-weight gain from day 0 to day 63 of the study was not significantly different between treatments (J, 0.79; NJ, 0.80; I, 0.80 kg). Sixty percent of the J calves and 53% of the NJ calves required four or more antibiotic treatments for respiratory disease while corresponding treatments were required for 97% of the I calves. The incidence of diarrhoea was significantly higher in both outdoor treatments compared to the I treatment. There was no significant difference in white blood cell counts or in serum immunoglobulin concentrations between treatments on days 0, 21, 42 and 63 or in in vitro interferon-γ production on day 63. It is concluded that using calf jackets on calves reared outdoors had no beneficial effect on calf performance or immune status. The incidence of respiratory disease was higher and diarrhoea incidence was lower in calves reared indoors compared with calves reared outdoors. There was no significant difference in incidences of diarrhoea and respiratory disease between the two outdoor treatments

Studies on restricted suckling in dual purpose and dairy breed cattle in Mexico

The aim of this thesis was to investigate the effects of rearing calves by restricted suckling (RS) compared to artificial rearing (AR) in dual purpose and dairy breed cattle in Mexico, milked once or three times a day, respectively. The following parameters were recorded during the first eight weeks after calving: social, abnormal and general behaviours and weight gain of Zebu crossbred and dairy calves; and milk yield, milk composition and udder health in the Zebu crossbred dams and udder health and milk let-down in the Holstein dams. RS reduced the abnormal behaviours of the calves and resulted in less foraging behaviours compared to AR. AR dairy calves consumed four-fold as much concentrate as RS dairy calves, whereas the amount of milk ingested was similar in the two treatments. A higher fat content in the milk ingested by RS calves compared to whole milk fed to AR calves, resulted in similar total ME intake from milk and concentrate in both treatments. Irrespective of type of animals RS resulted in similar weight gain in comparison to AR. Cow-calf separation five days after calving induced fewer indications of physiological stress in RS cows and calves. RS further increased the saleable and total (saleable and suckled) milk yield and decreased the fat content in saleable milk. RS improved udder health of the Zebu crossbred cows as judged according to elevated CMT scores and lower lactose content in AR cows. RS of Holstein dairy cows did not impair milk let-down and udder health tended to be improved according to the CMT. RS calves showed a front teat preference, which did not impair udder health according to similar CMT scores of front and rear teats. These studies indicate that RS is economically viable for the farmer as once daily milking combined with twice daily suckling considerably increased the saleable and total milk yield of Zebu crossbred cows and improved animal well-being