The genome sequence of the orange-tip butterfly, <i>Anthocharis cardamines</i> (Linnaeus, 1758).

We present a genome assembly from an individual female Anthocharis cardamines (the orange-tip; Arthropoda; Insecta; Lepidoptera; Pieridae). The genome sequence is 360 megabases in span. The majority (99.74%) of the assembly is scaffolded into 31 chromosomal pseudomolecules, with the W and Z sex chromosomes assembled. Gene annotation of this assembly on Ensembl has identified 12,477 protein coding genes

The genome sequence of the Squinting Bush Brown, <i>Bicyclus anynana</i> (Butler, 1879).

We present a genome assembly from an individual female Bicyclus anynana (the Squinting Bush Brown; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The genome sequence is 457.2 megabases in span. Most of the assembly is scaffolded into 28 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 16.1 kilobases in length

The genome sequence of the Rock Grayling, <i>Hipparchia semele</i> (Linnaeus, 1758).

We present a genome assembly from an individual female Hipparchia semele (the Rock Grayling; Arthropoda; Insecta; Lepidoptera; Nymphalidae). The genome sequence is 403.4 megabases in span. Most of the assembly is scaffolded into 30 chromosomal pseudomolecules, including the W and Z sex chromosomes. The mitochondrial genome has also been assembled and is 15.22 kilobases in length. Gene annotation of this assembly on Ensembl identified 17,540 protein coding genes

The genome sequence of the oyster mushroom, Pleurotus ostreatus ((Jacq.) P. Kummer, 1871).

We present a genome assembly from a specimen (the oyster mushroom; Basidiomycota; Agaricomycetes; Agaricales; Pleurotaceae). The genome sequence is 40.6 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules. Two mitochondrial genomes have been assembled, which are 73.1 and 9.3 kilobases in length

The genome sequence of the scale worm, Lepidonotus clava (Montagu, 1808)

We present a genome assembly from an individual Lepidonotus clava (scale worm; Annelida; Polychaeta; Phyllodocida; Polynoidae). The genome sequence is 1,044 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 15.6 kilobases in length.</ns3:p

The genome sequence of the turban top shell, Gibbula magus (Linnaeus, 1758)

We present a genome assembly from an individual Gibbula magus (the turban top shell; Mollusca; Gastropoda; Trochida; Trochidae). The genome sequence is 1,470 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.1 kilobases in length. Gene annotation of this assembly on Ensembl identified 41,167 protein coding genes.</ns3:p

The genome sequence of bittersweet, Solanum dulcamara L. (Solanaceae).

We present a genome assembly from an individual (bittersweet; Eudicot; Magnoliopsida; Solanales; Solanaceae). The genome sequence is 946.3 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial and plastid genomes have also been assembled, with lengths of 459.22 kilobases and 161.98 kilobases respectively

The genome sequence of the marsh skullcap, Scutellaria galericulata L.

We present a genome assembly from an individual (the marsh skullcap; Streptophyta; Magnoliopsida; Lamiales; Lamiaceae). The genome sequence is 328 megabases in span. Most of the assembly is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial and plastid genomes have also been assembled and have lengths of 326.5 kilobases and 152.6 kilobases respectively

High heteroplasmy is associated with low mitochondrial copy number and selection against non-synonymous mutations in the snail Cepaea nemoralis

Molluscan mitochondrial genomes are unusual because they show wide variation in size, radical genome rearrangements and frequently show high variation (>10%) within species. As progress in understanding this variation has been limited, we used whole genome sequencing of a six-generation matriline of the terrestrial snail Cepaea nemoralis, as well as whole genome sequences from wild-collected C. nemoralis, the sister species C. hortensis, and multiple other snail species to explore the origins of mitochondrial DNA (mtDNA) variation. The main finding is that a high rate of SNP heteroplasmy in somatic tissue was negatively correlated with mtDNA copy number in both Cepaea species. In individuals with under ten mtDNA copies per nuclear genome, more than 10% of all positions were heteroplasmic, with evidence for transmission of this heteroplasmy through the germline. Further analyses showed evidence for purifying selection acting on non-synonymous mutations, even at low frequency of the rare allele, especially in cytochrome oxidase subunit 1 and cytochrome b. The mtDNA of some individuals of Cepaea nemoralis contained a length heteroplasmy, including up to 12 direct repeat copies of tRNA-Val, with 24 copies in another snail, Candidula rugosiuscula, and repeats of tRNA-Thr in C. hortensis. These repeats likely arise due to error prone replication but are not correlated with mitochondrial copy number in C. nemoralis. Overall, the findings provide key insights into mechanisms of replication, mutation and evolution in molluscan mtDNA, and so will inform wider studies on the biology and evolution of mtDNA across animal phyla