1,061 research outputs found
Wage Dispersion and Decentralization of Wage Bargaining
This paper studies how decentralization of wage bargaining from sector to firm-level influences wage levels and wage dispersion. We use detailed panel data covering a period of decentralization in the Danish labor market. The decentralization process provides variation in the individual worker's wage-setting system that facilitates identification of the effects of decentralization. We find a wage premium associated with firm-level bargaining relative to sector-level bargaining, and that the return to skills is higher under the more decentralized wage-setting systems. Using quantile regression, we also find that wages are more dispersed under firm-level bargaining compared to more centralized wage-setting systems.wage bargaining, decentralization, wage dispersion
Wage Dispersion and Decentralization of Wage Bargaining
This paper studies how decentralization of wage bargaining from sector to firm level influences wage levels and wage dispersion. We use a detailed panel data set covering a period of decentralization in the Danish labor market. The decentralization process provides exogenous variation in the individual worker's wage-setting system that facilitates identification of the effects of decentralization. Consistent with predictions we find that wages are more dispersed under firm-level bargaining compared to more centralized wage-setting systems. However, the differences across wage-setting systems are reduced substantially when controlling for unobserved individual level heterogeneity.Wage bargaining; decentralization; panel data quantile regression
Comment on "On the -Anomaly in Betaine Calcium Chloride Dihydrate"
Recently, Hlinka and Ishibashi [J. Phys. Soc. Jpn. 67, 495 (1998)] discussed
the -anomaly in betaine calcium chloride dihydrate (BCCD) in a Landau-type
approach. We comment on the shortcomings of this approach and discuss the
-anomaly in the framework of a microscopical pseudo spin model based on a
realistic description of BCCD in terms of symmetry-adapted local modes.Comment: 2 pages, RevTex, submitted to J. Phys. Soc. Jp
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Optimisation of recombinant production of active human cardiac SERCA2a ATPase
Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge. We describe here, expression and purification optimisation of the human SERCA2a cardiac isoform of Ca2+ translocating ATPase, using Saccharomyces cerevisiae as the heterologous expression system of choice. Two different expression vectors were utilised, allowing expression of C-terminal fusion proteins with a biotinylation domain or a GFP- His8 tag. Solubilised membrane fractions containing the protein of interest were purified onto Streptavidin-Sepharose, Ni-NTA or Talon resin, depending on the fusion tag present. Biotinylated protein was detected using specific
antibody directed against SERCA2 and, advantageously, GFP-His8 fusion protein was easily traced during the purification steps using in-gel fluorescence. Importantly, talon resin affinity purification proved more specific than Ni-NTA resin for the GFP-His8 tagged protein, providing
better separation of oligomers present, during size exclusion chromatography. The optimised method for expression and purification of human cardiac SERCA2a reported herein, yields purified protein (> 90%) that displays a calcium-dependent thapsigargin-sensitive activity and is suitable for further biophysical, structural and physiological studies. This work provides support for the use of Saccharomyces cerevisiae as a suitable expression system for recombinant production of multi-domain eukaryotic membrane proteins
Thymulin (Zn-FTS) activity in protein-energy malnutrition : new evidence for interaction between malnutrition and infection on thymic function
The SPHERE data center: a reference for high contrast imaging processing
The objective of the SPHERE Data Center is to optimize the scientific return
of SPHERE at the VLT, by providing optimized reduction procedures, services to
users and publicly available reduced data. This paper describes our motivation,
the implementation of the service (partners, infrastructure and developments),
services, description of the on-line data, and future developments. The SPHERE
Data Center is operational and has already provided reduced data with a good
reactivity to many observers. The first public reduced data have been made
available in 2017. The SPHERE Data Center is gathering a strong expertise on
SPHERE data and is in a very good position to propose new reduced data in the
future, as well as improved reduction procedures.Comment: SF2A proceeding
Effects of ionizing radiations on proteins. Evidence of non-random fragmentations and a caution in the use of the method for determination of molecular mass
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