Einsatz von pflanzlichen Futterzusätzen zur Prophylaxe von E.coli - bedingten Durchfällen bei Absetzferkeln

The aim of this investigation was to examine the effect of different feed additives on prophylaxis of weaning diarrhoea.184 piglets were divided into 3 experimental groups and one control group by compensating randomisation. Feed additives were either herbs (“Herbenterosan”), oligogalacturonides (“Enteronid”) or lignocellulosis (“Agrocell”). Piglets were reared according to Council Regulation EC Nr. 889/2008 and weaned at 40 days of age. From day 4 after weaning until day 8 faeces of the piglets were examined every day to create a faecal score of each group (= group sum). Firm faeces were characterised as “0”, pasty faeces as “1” and liquid faeces as “2”. Live weight was examined regularly. Blood samples were collected twice for the analysis of haptoglobin and electrolytes. The Herbenterosan group showed the lowest group sum in faecal scoring. It was followed by the Enteronid group, the control group and the Agrocell group. No difference between groups was found in haptoglobin values. Electrolyte values (sodium, potassium and chloride) coincided with faecal scores: lower faecal score meant better values of electrolytes and conversely. The three different phytogenic feed additives were not able to prevent piglets from diarrhoea. The Herbenterosan group showed best values in faecal score and live weight gain

Quantitative determination of the faradiol esters in marigold flowers and extracts

An HPLC method is introduced for the precise quantitative determination of the anti-inflammatory active faradiol esters from marigold flowers and extracts. After a prepurification the HPLC analysis allows the proof of the quality of marigold flowers or C02-extracts used for pharmaceutical preparations

In vitro inhibitory effects of plant-derived by-products against Cryptosporidium parvum

Disposal of organic plant wastes and by-products from the food or pharmaceutical industries usually involves high costs. In the present study, 42 samples derived from such by-products were screened in vitro against Cryptosporidium parvum, a protozoan parasite that may contaminate drinking water and cause diarrhoea. The novel bioassay was previously established in the microtitre plate format. Human ileocaecal adenocarcinoma (HCT-8) cell cultures were seeded with C. parvum oocysts and parasite development was monitored by an indirect fluorescent antibody technique (IFAT) and microscopic assessment for clusters of secondary infection (CSI). Minimum inhibitory concentrations (MICs) and potential detrimental effects on the host cells were determined. An ethanolic extract from olive (Olea europaea) pomace, after oil pressing and phenol recovery, reproducibly inhibited C. parvum development (MIC = 250–500 μg mL−1, IC50 = 361 (279–438) μg mL−1, IC90 = 467 (398–615) μg mL−1). Accordingly, tyrosol, hydroxytyrosol, trans-coniferyl alcohol and oleuropein were selected as reference test compounds, but their contributions to the observed activity of the olive pomace extract were insignificant. The established test system proved to be a fast and efficient assay for identifying anti-cryptosporidial activities in biological waste material and comparison with selected reference compounds

Effect of Sustained Administration of Thymol on Its Bioaccessibility and Bioavailability in Rabbits

The objective of this study was the detection of thymol in rabbit plasma, tissues, large intestinal content, and faeces. Forty-eight rabbits were divided into control and experimental groups (thymol 250 mg/kg feed). Thymol was administered for 21 days and then withdrawn for 7 days. Concentration of thymol in the intestinal wall (IW) was significantly higher than in plasma (p < 0.05) and liver (p < 0.05); in the kidneys it was significantly higher than in plasma (p < 0.05) and liver (p < 0.05) during thymol addition. Thymol in IW was significantly higher than in plasma also after withdrawal (p < 0.01). Significant correlation (rs = −1.000, p < 0.01) between IW and plasma points to the intensive absorption of thymol from the intestine, while the correlation between plasma and liver (rs = 0.786, p < 0.05) indicates intensive biotransformation and excretion processes in liver. Significant correlation between liver and kidney (rs = 0.738, p < 0.05) confirms the intensive metabolism of thymol in the kidney. During the withdrawal period, thymol was detected above trace amounts only in faeces, and was significantly higher than in the colon during both periods (p < 0.01). Results show intensive biotransformation of thymol in the rabbit organism

Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits.

To find natural feed additives with a beneficial effect on rabbit health, thymol alone and in combination with Enterocin M were administered in drinking water for 42 days (35 -77 days of age). A total of 48 rabbits based on their weight were randomly divided into four experimental groups: C – control (basal diet), T – thymol (250 mg/L), E – Enterocin M (Ent M) (50 μL/animal/day), T + E (thymol with Ent M). Ent M (p < .05) and thymol (p < .01) separately decreased malondialdehyde in the liver. Thymol separately and in combination significantly increased phagocytic activity in the blood (p = .0051) and lactic acid in the caecum (p = .0142) and decreased coagulase-positive staphylococci in the caecum (p = .0329). Ent M separately and in combination increased immunoglobulin A content in the jejunal wall (p = .002) and decreased coliform bacteria in faeces (p = .0002). Thymol and Ent M application separately or in combination improved the antioxidant and immune response of rabbits and demonstrated an antibacterial effect

Effect of thymol and Enterocin M administration on biochemical, antioxidant and immunological parameters, small intestinal morphology and microbiota in rabbits

To find natural feed additives with a beneficial effect on rabbit health, thymol alone and in combination with Enterocin M were administered in drinking water for 42 days (35 -77 days of age). A total of 48 rabbits based on their weight were randomly divided into four experimental groups: C - control (basal diet), T - thymol (250 mg/L), E - Enterocin M (Ent M) (50 lL/animal/day), TthornE (thymol with Ent M). Ent M (p <.05) and thymol (p <.01) separately decreased malondialdehyde in the liver. Thymol separately and in combination significantly increased phagocytic activity in the blood (p = .0051) and lactic acid in the caecum (p =0142) and decreased coagulase-positive staphylococci in the caecum (p =.0329). Ent M separately and in combination increased immunoglobulin A content in the jejunal wall (p =.002) and decreased coliform bacteria in faeces (p =.0002). Thymol and Ent M application separately or in combination improved the antioxidant and immune response of rabbits and demonstrated an antibacterial effect