Isothermal titration calorimetry results and the curving fitting between peptide ligands and Shank1 PDZ protein.

<p>(A) The titration of ligands <b>p1</b>, <b>p2</b> and <b>p3</b> to Shank1 PDZ protein (A1, A2 and A3 are for <b>p1</b>, <b>p2</b> and <b>p3</b> respectively). (A1) [<b>p1</b>] = 4 mM, [Shank1 PDZ] = 400 μM; (A2) [<b>p2</b>] = 326 μM, [Shank1 PDZ] = 43 μM; (A3) [<b>p3</b>] = 354 μM, [Shank1 PDZ] = 35 μM. For the binding pair <b>p1</b> ligand and Shank1 PDZ, the affinity was very low so that protein concentration was adjusted higher to get accuracy affinity data. (B)The titration of diluted <b>p2</b>, <b>p3</b> and dimeric peptide with changed linker length to Shank1 PDZ protein. (B1) [<b>p2</b>] = 194 μM, [Shank1 PDZ] = 0.018 μM; (B2) [<b>p3</b>] = 160 μM, [Shank1 PDZ] = 16 μM; (B3) [dimeric peptide BM(PEG)₃] = 600 μM, [Shank1 PDZ] = 53 μM.</p

Native mass spectrometric analysis of Shank1 PDZ protein with peptide dendrimers in 1:1 mixture.

<p>(A) Deconvoluted native mass spectrum of Shank1 PDZ protein without ligands, 130 μM in 150 mM ammonium acetate. (B-D) Deconvoluted mass spectra of Shank1 PDZ protein and peptide ligands <b>p1</b>, <b>p2</b> and <b>p3</b> mixed at 1:1 ratio (B, C and D are for <b>p1</b>, <b>p2</b> and <b>p3</b> respectively). In all three spectra, protein−peptide complexes with 1:1 stoichiometry were observed (indicated by the arrows). [Shank1 PDZ] = [peptide] = 65 μM.</p

Surface plasmon resonance demonstrated ligands p1, p2 and p3 binding to Shank1 PDZ protein immobilized on the sensor chip.

<p>(A, B and C are for <b>p1</b>, <b>p2</b> and <b>p3</b> respectively). (A) [<b>p1</b>] = 11–346 μM; (B) [<b>p2</b>] = 10–320 μM; (C) [<b>p3</b>] = 5–168 μM. Dash lines in (B) & (C) were kinetics fitting used 1:1 Langmuir binding model for dimer <b>p2</b> and trimer <b>p3</b>.</p

Native mass spectrometric analysis of Shank1 PDZ protein with peptide dendrimer in 4:1 mixture.

<p>(A) Deconvoluted native mass spectrum of Shank1 PDZ protein without ligands, 91 μM in 150 mM ammonium acetate. (B-D) Deconvoluted mass spectra of Shank1 PDZ protein and peptide ligands <b>p1</b>, <b>p2</b> and <b>p3</b> mixed at 4:1 ratio (B, C and D are for <b>p1</b>, <b>p2</b> and <b>p3</b> respectively). Similarly, only protein−peptide complexes with 1:1 stoichiometry were observed (indicated by the solid line arrows). [Shank1 PDZ] = 45 μM; [peptide] = 11 μM. The peak at 13757 (indicated by * in the spectra) is from an impurity protein that co-purified with Shank1 PDZ protein in this batch. The protein peaks at around 14900 is from the Shank PDZ protein with the first methionine removed and the peak at around 15076 is from the Shank PDZ protein with the first methionine; both peaks are present in the spectrum of protein itself, so they are not relevant to the protein−peptide complex.</p

Isothermal titration calorimetry of ligands p1, p2 and p3 to Shank3 PDZ protein (A, B and C are for p1, p2 and p3 respectively).

<p>(A) [<b>p1</b>] = 406 μM, [Shank3 PDZ] = 40μM; (B) [<b>p2</b>] = 400 μM, [Shank3 PDZ] = 30 μM; (C) [<b>p3</b>] = 315 μM, [Shank3 PDZ] = 34 μM.</p

Peptide dendrimers for Shank PDZ as mimics for the clustered ligand in βPIX.

<p>The design of monomeric, dimeric and trimeric peptides <b>p1</b>, <b>p2</b> and <b>p3</b> to mimic the trimer structure ofβPIX.</p