How Much Bean Hemagglutinin Is Safe for Human Consumption?

How Much Bean Hemagglutinin Is Safe for Human Consumption

Additional file 1 of Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Additional file 1: Fig S1. The conserved BURP domain and signal peptide of BURP proteins in jujube. The phylogenetic tree of 17 BURP proteins is shown using MEGA-X with the neighbor-joining (NJ) method. The red filled boxed represent the BURP domain, and the green filled boxes represent the signal peptide

Additional file 5 of Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Additional file 5: Table S2. Primer pairs used in RT-PCR and qRT-PCR analysis

Additional file 3 of Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Additional file 3: Table S1. Number of cis-elements in putative promoters of ZjBURPs

Additional file 2 of Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Additional file 2: Fig S2. The conserved amino acid residues in BURP domain of jujube sequences. Multialignment of 17 ZjBURP proteins using DNAMAN v. 10.3.3.126. The black-, pink-, and aquamarine-shaded amino acids represented the homology level with 100%, over 75%, and over 50%, respectivel

Additional file 4 of Identification and expression analysis of BURP domain-containing genes in jujube and their involvement in low temperature and drought response

Additional file 4: Fig S3. The original, uncropped gel of RT-PCR analysis of ZjBURPs in three tissues. The red box denoted the region of the original gel. The bands in the F, YF, and L row represented ZjBURPs expression in flower, young fruit, and leaf, respectively. 1-9: ZjBNM1, 2, 3, 4, 5, 6, 7, 8, and 9; 10-14: ZjBURP1, 2, 3, 4, and 5; 15-17: ZjPG1, 2, and 3; 18: Zj26S-2

Dual recording from pericytes and endothelia during AngII exposure, cells associated or spatially separated.

<p><b>A.</b> Resting potential of pericytes and endothelia that are associated on the DVR wall (as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154948#pone.0154948.g005" target="_blank">Fig 5A</a>). Endothelial cells were significantly hyperpolarized relative to the pericytes (paired t-test, n = 6, ** P < 0.01). <b>B.</b> Simultaneous endothelial and pericyte resting membrane potentials from panel A superimposed on the line of identity. By linear regression, values were highly correlated (DF = 5, F = 221, R = 0.99, P < 0.001). <b>C.</b> Resting potential of pericytes and endothelia dissociated from one another (as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154948#pone.0154948.g005" target="_blank">Fig 5B</a>). Resting potentials were not significantly different (n = 7). <b>D.</b> Simultaneous endothelial and pericyte resting membrane potentials from panel C superimposed on the line of identity. A significant correlation was not observed (DF = 6, F = 0.13, R = 0.16, P = 0.73).</p

Descending Vasa Recta Endothelial Membrane Potential Response Requires Pericyte Communication - Fig 4

<p><b>A.</b> Single examples of AngII (10 nM) stimulated membrane potentials of paired pericytes isolated from endothelium, either in contact (<b>A</b>, P-P) or without contact (<b>B</b>, PxP). In each example the individual data points are superimposed upon the dashed line of identity. <b>C.</b> Summary of mean ± SEM of correlation coefficients for P-P and PxP configurations (n = 8 each; **, P < 0.01). Other summaries are provided in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0154948#pone.0154948.t001" target="_blank">Table 1</a></b>.</p

Dual recording from pericyte and endothelium during AngII exposure and gap junction blockade.

<p><b>A.</b> Photomicrograph of the dual pericyte, endothelial preparation that yielded the recording in panel B. The black bar = 10 microns. <b>B.</b> Electrophysiological record; square wave depolarizations from -80 to -30 mV were imposed at regular intervals on a voltage clamped pericyte. The endothelial response during pericyte depolarization (top) and the pericyte command potential (bottom) are shown before, during and after application of heptanol (2 mM). <b>C.</b> Summary of experiments similar to that shown in panel B. The average membrane potential deviation of the endothelium is shown before, during and after washout of heptanol, which reversibly attenuated conduction (mean ± SEM, n = 6). Repeated measures ANOVA, DF = 17, F = 19.5, P < 0.001. By Holm-Sidak multiple comparison; * P < 0.05 baseline vs heptanol, # P < 0.05, heptanol vs washout. <b>D.</b> Summary of experiments similar to that shown in panel B, but with 18BGRA (20 microM) as the gap junction blocker (mean ± SEM, n = 8). Repeated measures ANOVA, DF = 23, F = 38.6, P < 0.001. By Holm-Sidak multiple comparison; ** P < 0.01 baseline vs 18BGRA, # P < 0.05 18BGRA vs washout.</p

Summary of linear regressions fit to dual pericyte membrane potential records during vasoconstrictor application.

<p>Summary of linear regressions fit to dual pericyte membrane potential records during vasoconstrictor application.</p