67 research outputs found
Surgical excision promotes tumor growth and metastasis by promoting expression of MMP-9 and VEGF in a breast cancer model
Surgery is still the main curative therapeutic modality for breast cancer. Although surgery often results in the successful removal of the primary tumor, its process could increase the risk of metastases of residual cancer cells. Understanding of the connection between breast cancer metastasis and surgical wound will lead to the establishment of a proper treatment strategy for postoperative cancer patient. Aim: To study the influence of surgical procedure on the metastasis of primary breast cancer. Methods: We established MDA-MB-435 human breast cancer xenograft model. Levels of Pro-matrix metalloproteinase 9 (Pro-MMP-9) and vascular endothelial growth factor (VEGF) in host serum and tumors were tested at different time points with ELISA and zymography and correlated to tumor growth and postoperative metastasis. Results: Our study demonstrated surgical wound had promoting effect on tumor growth and pulmonary metastasis of human breast cells, if tumor cells remain in bodies. This effect might be related to the postoperative interaction of cancer and host cells, which resulted in expression of Pro-MMP-9. Surgical process could also increase the VEGF expression in tumor tissues. Conclusions: Surgical wound-produced host Pro-MMP-9 and tumor cell VEGF might be important mediators leading to metastasis of residual breast cancer after surgery.Π₯ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ Π»Π΅ΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎ ΡΠΈΡ
ΠΏΠΎΡ ΠΎΡΡΠ°Π΅ΡΡΡ Π³Π»Π°Π²Π½ΡΠΌ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ΠΎΠΌ ΠΏΡΠΈ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ°ΠΊΠΎΠΌ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ
ΠΆΠ΅Π»Π΅Π·Ρ. Π₯ΠΎΡΡ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ΄Π°Π»Π΅Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² Π±ΠΎΠ»ΡΡΠΈΠ½ΡΡΠ²Π΅ ΡΠ»ΡΡΠ°Π΅Π² Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ, Π² ΡΠΎ ΠΆΠ΅ Π²ΡΠ΅ΠΌΡ ΠΎΠ½ΠΎ ΠΌΠΎΠΆΠ΅Ρ
ΡΠ²Π΅Π»ΠΈΡΠΈΡΡ ΡΠΈΡΠΊ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΡΠΈ ΡΠ°ΠΊΠ΅
ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠ°Π½ΠΎΠΉ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ Π²ΡΠ±ΡΠ°ΡΡ Π½Π°ΠΈΠ»ΡΡΡΡΡ ΡΡΡΠ°ΡΠ΅Π³ΠΈΡ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ. Π¦Π΅Π»Ρ:
ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π° Π½Π° ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠ΅ΡΠΎΠ΄Ρ: Π±ΡΠ»Π° ΡΠΎΠ·Π΄Π°Π½Π° ΠΌΠΎΠ΄Π΅Π»Ρ ΡΠ°ΠΊΠ°
ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ° Π² Π²ΠΈΠ΄Π΅ ΠΊΡΠ΅Π½ΠΎΡΡΠ°Π½ΡΠΏΠ»Π°Π½ΡΠ°ΡΠ° MDA-MB-435. Π£ΡΠΎΠ²Π΅Π½Ρ Pro-ΠΌΠ°ΡΡΠΈΡΠ½ΠΎΠΉ ΠΌΠ΅ΡΠ°Π»Π»ΠΎΠΏΡΠΎΡΠ΅ΠΈΠ½Π°Π·Ρ 9
(Pro-MMP-9) ΠΈ ΡΠ°ΠΊΡΠΎΡΠ° ΡΠΎΡΡΠ° ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΡ ΡΠΎΡΡΠ΄ΠΎΠ² (VEGF) Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΡΠ΅ΡΠΈΠΏΠΈΠ΅Π½ΡΠ° ΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈΡΡ Π² ΡΠ°Π·Π½ΡΠ΅
Π²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΏΡΠΎΠΌΠ΅ΠΆΡΡΠΊΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ELISA ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°- ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π° ΠΈ Π·ΠΈΠΌΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π½Π°Π»ΠΈΡΠΈΠ΅ ΠΊΠΎΡΠ΅Π»Π»ΡΡΠΈΠΈ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ,
ΡΠΎΡΡΠΎΠΌ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·Π°ΠΌΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ°Π½Π° ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ
ΡΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠ»ΠΎΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ Π΅Π΅ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π² Π»Π΅Π³ΠΊΠΈΠ΅ Π² ΡΠ»ΡΡΠ°Π΅, ΠΊΠΎΠ³Π΄Π° ΠΏΡΠΈ ΡΠ΄Π°Π»Π΅Π½ΠΈΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Π² ΡΠ°Π½Π΅
ΠΎΡΡΠ°ΡΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠ΅ ΠΊΠ»Π΅ΡΠΊΠΈ. ΠΡΠΎ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΌ Π²Π·Π°ΠΈΠΌΠΎΠ΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΈ ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΡ
ΠΈΡ
Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ, ΠΊΠΎΡΠΎΡΠΎΠ΅ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Pro-MMP-9. Π£Π΄Π°Π»Π΅Π½ΠΈΠ΅ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΌΠΎΠΆΠ΅Ρ ΡΠ°ΠΊΠΆΠ΅ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΠΎΠ²Π°ΡΡ
ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ VEGF ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ. ΠΡΠ²ΠΎΠ΄Ρ: Pro-MMP-9, ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΠΉ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ,
ΠΎΠΊΡΡΠΆΠ°ΡΡΠΈΠΌΠΈ ΠΏΠΎΡΡΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΡ ΡΠ°Π½Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ VEGF, ΠΏΡΠΎΠ΄ΡΡΠΈΡΡΠ΅ΠΌΡΠΉ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΠΌΠΈ ΠΊΠ»Π΅ΡΠΊΠ°ΠΌΠΈ, ΠΌΠΎΠ³ΡΡ Π±ΡΡΡ Π²Π°ΠΆΠ½ΡΠΌΠΈ
ΠΌΠ΅Π΄ΠΈΠ°ΡΠΎΡΠ°ΠΌΠΈ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎΡΠ»Π΅ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²Π°
Predictions of total and total reaction cross sections for nucleon-nucleus scattering up to 300 MeV
Total reaction cross sections are predicted for nucleons scattering from various nuclei. Projectile energies to 300 MeV are considered. So also are mass variations of those cross sections at selected energies. All predictions have been obtained from coordinate space optical potentials formed by full folding effective two-nucleon (NN) interactions with one body density matrix elements (OBDME) of the nuclear ground states. Good comparisons with data result when effective NN interactions defined by medium modification of free NN t matrices are used. Coupled with analyses of differential cross sections, these results are sensitive to details of the model ground states used to describe nuclei
RIG-I detects HIV-1 infection and mediates type I interferon response in human macrophages from patients with HIV-1-associated neurocognitive disorders
Effects of watering regime and depth of burial on seedling emergence of four dominant psammophytes in the Mu Us sandy land, Inner Mongolia, China, and relevance to revegetation of a desertified region
Interpretation of human and robot emblematic gestures: How do they differ?
Human gestures are often directly adopted in gesture design for humanoid robots when human-robot interaction is considered, but the interpretations of human and robot gestures can vary due to the structural difference between human and robot and due to people's perceptual difference of human and robot. In this paper, we investigate how people interpret human and robot gestures differently using emblematic gestures, a type of gesture with specific verbal translations. Nine commonly used emblems performed by a human were evaluated by 32 participants, and the nine emblems performed by a NAO humanoid robot were evaluated by another 32 participants. We compared participants' interpretations of the human and robot emblems systematically. The results indicate that the multimessage gesture and multigesture message characteristics of human emblems also exist in robot emblems. However, participants' recognition rates of robot emblems are lower than those of human emblems in many cases. The human and robot emblems can also vary in interpretations and corresponding percentages. Some in
Microstructures of electroless NiβP alloy deposits and their transformation sequences during post-deposition annealing
Cytological identification of an <i>Aegilops variabilis</i> chromosome carrying stripe rust resistance in wheat
Existence of positive ground state solutions of SchrΓΆdingerβPoisson system involving negative nonlocal term and critical exponent on bounded domain
Progress in research on applying Sijunzi Decoction (εεεζ±€) in treating digestive malignant tumor
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