Effects of PHC on the expression of p-IκB, IκB and NF-κB in heart tissues after I/R.

<p>Myocardial tissues were obtained and the expression of p-IκB, IκB and NF-κB were analyzed by western blot. The band densities were normalized to GAPDH or Histone H3. Data were expressed as means±SD (n = 5 in each group). ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R (ns means no significant difference).</p

Long-term effect of H-PHC on myocardial infarct and cardiac function.

<p>The rats were given a single injection of H-PHC 30 min before the surgery, and then the rats were subjected to ischemia/reperfusion. After 7 days, infarct size <b>(A)</b> and hemodynamic changes <b>(B)</b> were measured. Scale bars represent 10 mm. Data were expressed as means±SD (n = 6 in each group). ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R.</p

Effects of PHC on cardiac function.

<p><b>(A)</b> EF. <b>(B)</b> FS. <b>(C)</b> LVEDP. <b>(D)</b> LVSP. Values were mean±SD (n = 6 in each group). ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R (ns means no significant difference).</p

Effects of PHC on myocardial injury and oxidative stress.

<p>The rats were subjected to 30 min ischemia and 3 h reperfusion. The levels of CK <b>(A)</b>, AST <b>(B)</b>, LDH <b>(C)</b>, total NO <b>(D)</b>, ROS <b>(E)</b>, MDA <b>(F)</b> and total GSH <b>(G)</b> were measured after 3h reperfusion. The activities of SOD <b>(H)</b>, CAT <b>(I)</b> and GSH-Px <b>(J)</b> were determined. Values were mean±SD (n = 6 in each group). #<i>P</i><0.05 and ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R (ns means no significant difference).</p

Histological analysis of myocardial infarction area.

<p>Infarct size was measured after 30 min ischemia and 3 h reperfusion. Scale bars represent 10 mm. Representative images were shown. Data are expressed as mean±SD (n = 6 in each group). ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R.</p

Effects of PHC on cardiomyocyte apoptosis.

<p>After reperfusion, myocardial tissues were subjected to TUNEL staining. Representative images <b>(A)</b> were photographed and showed. Images in <b>(B)</b> are insets from red squares in <b>(A)</b>. The red arrows pointed to the cells that presented typical apoptosis features. The apoptotic rates in each group were calculated <b>(C)</b>. Scale bars represent 20 μm. Values are mean±SD (n = 6 in each group). ##<i>P</i><0.01 vs. Sham; *<i>P</i><0.05 and **<i>P</i><0.01 vs. I/R.</p

Data_Sheet_1_Prevalence and phylogenetic analysis of human enteric emerging viruses in porcine stool samples in the Republic of Korea.docx

Emerging infectious diseases (EID) in humans and animals are proving to be a serious health concern. This study investigated the prevalence of emerging or re-emerging human enteric viruses in porcine stools and swabs. Eleven enteric EID viruses were selected as target viruses for the current study and ranked based on their impact on public health and food safety: enterovirus (EV), hepatitis E virus, norovirus GI and GII, sapovirus (SaV), adenovirus (AdV), astrovirus, rotavirus, hepatitis A virus, aichivirus, and bocavirus. Using real-time RT-PCR or real-time PCR, EID viruses were detected in 129 (86.0%) of 150 samples. The most prevalent virus was EV, which was detected in 68.0% of samples, followed by AdV with a detection rate of 38.0%. In following sequencing and phylogenetic analyses, 33.0% (58/176) of the detected viruses were associated with human enteric EID viruses, including AdV-41, coxsackievirus-A2, echovirus-24, and SaV. Our results show that porcine stools frequently contain human enteric viruses, and that few porcine enteric viruses are genetically related to human enteric viruses. These findings suggest that enteric re-emerging or EID viruses could be zoonoses, and that continuous monitoring and further studies are needed to ensure an integrated “One Health” approach that aims to balance and optimize the health of humans, animals, and ecosystems.</p

Data_Sheet_2_Prevalence and phylogenetic analysis of human enteric emerging viruses in porcine stool samples in the Republic of Korea.xlsx

Emerging infectious diseases (EID) in humans and animals are proving to be a serious health concern. This study investigated the prevalence of emerging or re-emerging human enteric viruses in porcine stools and swabs. Eleven enteric EID viruses were selected as target viruses for the current study and ranked based on their impact on public health and food safety: enterovirus (EV), hepatitis E virus, norovirus GI and GII, sapovirus (SaV), adenovirus (AdV), astrovirus, rotavirus, hepatitis A virus, aichivirus, and bocavirus. Using real-time RT-PCR or real-time PCR, EID viruses were detected in 129 (86.0%) of 150 samples. The most prevalent virus was EV, which was detected in 68.0% of samples, followed by AdV with a detection rate of 38.0%. In following sequencing and phylogenetic analyses, 33.0% (58/176) of the detected viruses were associated with human enteric EID viruses, including AdV-41, coxsackievirus-A2, echovirus-24, and SaV. Our results show that porcine stools frequently contain human enteric viruses, and that few porcine enteric viruses are genetically related to human enteric viruses. These findings suggest that enteric re-emerging or EID viruses could be zoonoses, and that continuous monitoring and further studies are needed to ensure an integrated “One Health” approach that aims to balance and optimize the health of humans, animals, and ecosystems.</p

BMP-6 promotes E-cadherin expression through repressing δEF1 in breast cancer cells-0

<p><b>Copyright information:</b></p><p>Taken from "BMP-6 promotes E-cadherin expression through repressing δEF1 in breast cancer cells"</p><p>http://www.biomedcentral.com/1471-2407/7/211</p><p>BMC Cancer 2007;7():211-211.</p><p>Published online 13 Nov 2007</p><p>PMCID:PMC2217560.</p><p></p>cancer cells were detected by RT-PCR analysis. GAPDH was used as an internal control. (b) The agarose gel electrophoresis data was quantified by UV band scanning. Data represent three independent experiments

BMP-6 promotes E-cadherin expression through repressing δEF1 in breast cancer cells-7

<p><b>Copyright information:</b></p><p>Taken from "BMP-6 promotes E-cadherin expression through repressing δEF1 in breast cancer cells"</p><p>http://www.biomedcentral.com/1471-2407/7/211</p><p>BMC Cancer 2007;7():211-211.</p><p>Published online 13 Nov 2007</p><p>PMCID:PMC2217560.</p><p></p>lysis in MDA-MB-231 cells, using an unrelated anti-FLAG antibody or an anti-ZEB antibody directed against the N-terminal epitopes of δEF1. The amplified human E-cadherin promoter fragment is shown (-175/+21). (b) For quantitative CHIP assay, MDA-MB-231 cells were treated with or without 200 ng/ml rhBMP-6. Cell lysates were collected after 72 h. The IP was performed using anti-ZEB antibody (10 μg) with anti-FLAG antibody (10 μg) as a negative control. DNA fragments containing the E-cadherin promoter region (-175/+21) were amplified by quantitative PCR from anti-ZEB and anti-FLAG immunoprecipitated samples. Data represent three independent experiments. * indicates p < 0.05 in unpaired t-test when compared with un-treated group