Sparsity-Based Two-Dimensional DOA Estimation for Co-Prime Planar Array via Enhanced Matrix Completion

In this paper, the two-dimensional (2-D) direction-of-arrival (DOA) estimation problem is explored for the sum-difference co-array (SDCA) generated by the virtual aperture expansion of co-prime planar arrays (CPPA). Since the SDCA has holes, this usually causes the maximum virtual aperture of CPPA to be unavailable. To address this issue, we propose a complex-valued, sparse matrix recovery-based 2-D DOA estimation algorithm for CPPA via enhanced matrix completion. First, we extract the difference co-arrays (DCA) from SDCA and construct the co-array interpolation model via nuclear norm minimization to initialize the virtual uniform rectangular array (URA) that does not contain the entire rows and columns of holes. Then, we utilize the shift-invariance structure of the virtual URA to construct the enhanced matrix with a two-fold Hankel structure to fill the remaining empty elements. More importantly, we apply the alternating direction method of the multipliers (ADMM) framework to solve the enhanced matrix completion model. To reduce the computational complexity of the traditional vector-form, sparse recovery algorithm caused by the Kronecker product operation between dictionary matrices, we derive a complex-valued sparse matrix-recovery model based on the fast iterative shrinkage-thresholding (FISTA) method. Finally, simulation results demonstrate the effectiveness of the proposed algorithm

Sparsity-Based Two-Dimensional DOA Estimation for Co-Prime Planar Array via Enhanced Matrix Completion

In this paper, the two-dimensional (2-D) direction-of-arrival (DOA) estimation problem is explored for the sum-difference co-array (SDCA) generated by the virtual aperture expansion of co-prime planar arrays (CPPA). Since the SDCA has holes, this usually causes the maximum virtual aperture of CPPA to be unavailable. To address this issue, we propose a complex-valued, sparse matrix recovery-based 2-D DOA estimation algorithm for CPPA via enhanced matrix completion. First, we extract the difference co-arrays (DCA) from SDCA and construct the co-array interpolation model via nuclear norm minimization to initialize the virtual uniform rectangular array (URA) that does not contain the entire rows and columns of holes. Then, we utilize the shift-invariance structure of the virtual URA to construct the enhanced matrix with a two-fold Hankel structure to fill the remaining empty elements. More importantly, we apply the alternating direction method of the multipliers (ADMM) framework to solve the enhanced matrix completion model. To reduce the computational complexity of the traditional vector-form, sparse recovery algorithm caused by the Kronecker product operation between dictionary matrices, we derive a complex-valued sparse matrix-recovery model based on the fast iterative shrinkage-thresholding (FISTA) method. Finally, simulation results demonstrate the effectiveness of the proposed algorithm

Binding Interactions of Zinc Cationic Porphyrin with Duplex DNA: From B-DNA to Z-DNA

Recognition of unusual left-handed Z-DNA by specific binding of small molecules is crucial for understanding biological functions in which this particular structure participates. Recent investigations indicate that zinc cationic porphyrin (ZnTMPyP4) is promising as a probe for recognizing Z-DNA due to its characteristic chiroptical properties upon binding with Z-DNA. However, binding mechanisms of the ZnTMPyP4/Z-DNA complex remain unclear. By employing time-resolved UV-visible absorption spectroscopy in conjunction with induced circular dichroism (ICD), UV-vis, and fluorescence measurements, we examined the binding interactions of ZnTMPyP4 towards B-DNA and Z-DNA. For the ZnTMPyP4/Z-DNA complex, two coexisting binding modes were identified as the electrostatic interaction between pyridyl groups and phosphate backbones, and the major groove binding by zinc(II) coordinating with the exposed guanine N7. The respective contribution of each mode is assessed, allowing a complete scenario of binding modes revealed for the ZnTMPyP4/Z-DNA. These interaction modes are quite different from those (intercalation and partial intercalation modes) for the ZnTMPyP4/B-DNA complex, thereby resulting in explicit differentiation between B-DNA and Z-DNA. Additionally, the binding interactions of planar TMPyP4 to DNA were also investigated as a comparison. It is shown that without available virtual orbitals to coordinate, TMPyP4 binds with Z-DNA solely in the intercalation mode, as with B-DNA, and the intercalation results in a structural transition from Z-DNA to B-ZNA. These results provide mechanistic insights for understanding ZnTMPyP4 as a probe of recognizing Z-DNA and afford a possible strategy for designing new porphyrin derivatives with available virtual orbitals for the discrimination of B-DNA and Z-DNA

Efficacy and Safety of PD-1/PD-L1 Checkpoint Inhibitors versus Anti-PD-1/PD-L1 Combined with Other Therapies for Tumors: A Systematic Review

Objective: In recent years, the anti-programmed cell death protein-1 and its ligand (PD-1/PD-L1) or combination therapies have been recommended as an alternative emerging choice of treatment for oncology patients. However, the efficacy and adverse events of different combination strategies for the treatment of tumors remain controversial. Methods: PubMed, Embase, Cochrane Library, the American Society of Clinical Oncology (ASCO), and the European Society of Medicine Oncology (ESMO) were searched from database inception until 16 February 2022. The endpoints of objective response rate (ORR), disease control rate (DCR), overall survival (OS), progression-free survival (PFS), and adverse events (AEs) were analyzed from different treatment schemes and tumor types. The protocol was registered in PROSPERO (CRD42022328927). Results: This meta-analysis included forty-eight eligible studies. Combination therapy has improved ORR (RR = 1.40, p p p p = 0.117). Besides, combination treatment strategies are more toxic in any grade AEs (RR = 1.13, p p < 0.001). Conclusions: Treatment with PD-1/PD-L1 inhibitors in combination with other antitumor therapies improve patients’ ORR, DCR, and PFS compared to anti-PD-1/PD-L1. However, it is regrettable that there is no benefit to OS and an increased risk of AEs in combinatorial therapies

Additional file 1 of Relationship between sarcopenia/paravertebral muscles and the incidence of vertebral refractures following percutaneous kyphoplasty: a retrospective study

Additional file 1

Additional file 2 of Relationship between sarcopenia/paravertebral muscles and the incidence of vertebral refractures following percutaneous kyphoplasty: a retrospective study

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LINC00482 sponged miR-2467-3p to promote bone metastasis of prostate cancer through activating Wnt/β-catenin signaling pathway

This study was designed to investigate the biological functions of LINC00482 in prostate cancer (PCa) with bone metastasis. TCGA dataset of PCa was applied for LINC00482 expression analysis and real time PCR was used to verify the expression level of LINC00482 in PCa tissues as well as PCa bone metastatic tissues. To detect the biological functions of LINC00482 in vitro, various assays were used including CCK-8, EdU, colony formation and transwell assays. The biological functions of LINC00482 were also identified in vivo by inoculating PCa cells into the left cardiac ventricle of mice, followed by evaluating the osteolytic lesions and osteolytic score. In addition, Starbase and Lncbase databases were applied for predicting the potential target miRNA of LINC00482, while TargetScan and Starbase databases were used for predicting the potential target of miRNA. The luciferase reporter assay was utilized to determine the interactions among these molecules and western blotting was employed to verified the targeted proteins. Results showed that high expression level of LINC00482 was observed in bone metastatic PCa tissues and associated with PCa progression. Silencing of LINC00482 inhibited cell proliferation, migration and invasion in PCa. Furthermore, LINC00482 was proved to act as a competing endogenous RNA by sponging miR-2467-3p to activate Wnt/β-catenin signaling pathway, which may be a promising therapeutic target for PCa with bone metastasis

Multidimensional analysis to elucidate the possible mechanism of bone metastasis in breast cancer

Abstract Background Breast cancer (BC) patients tend to suffer from distant metastasis, especially bone metastasis. Methods All the analysis based on open-accessed data was performed in R software, dependent on multiple algorithms and packages. The RNA levels of specific genes were detected using quantitative Real-time PCR as a method of detecting the RNA levels. To assess the ability of BC cells to proliferate, we utilized the CCK8 test, colony formation, and the 5-Ethynyl-20-deoxyuridine assay. BC cells were evaluated for invasion and migration by using Transwell assays and wound healing assays. Results In our study, we identified the molecules involved in BC bone metastasis based on the data from multiple BC cohorts. Then, we comprehensively investigated the effect pattern and underlying biological role of these molecules. We found that in the identified molecules, the EMP1, ACKR3, ITGA10, MMP13, COL11A1, and THY1 were significantly correlated with patient prognosis and mainly expressed in CAFs. Therefore, we explored the CAFs in the BC microenvironment. Results showed that CAFs could activate multiple carcinogenic pathways and most of these pathways play an important role in cancer metastasis. Meanwhile, we noticed the interaction between CAFs and malignant, endothelial, and M2 macrophage cells. Moreover, we found that CAFs could induce the remodeling of the BC microenvironment and promote the malignant behavior of BC cells. Then, we identified MMP13 for further analysis. It was found that MMP13 can enhance the malignant phenotype of BC cells. Meanwhile, biological enrichment and immune infiltration analysis were conducted to present the effect pattern of MMP13 in BC. Conclusions Our result can improve the understanding of researchers on the underlying mechanisms of BC bone metastasis

Characterization of oral bacterial and fungal microbiome in recovered COVID-19 patients

Abstract COVID-19 has emerged as a global pandemic, challenging the world’s economic and health systems. Human oral microbiota comprises the second largest microbial community after the gut microbiota and is closely related to respiratory tract infections; however, oral microbiomes of patients who have recovered from COVID-19 have not yet been thoroughly studied. Herein, we compared the oral bacterial and fungal microbiota after clearance of SARS-CoV-2 in 23 COVID-19 recovered patients to those of 29 healthy individuals. Our results showed that both bacterial and fungal diversity were nearly normalized in recovered patients. The relative abundance of some specific bacteria and fungi, primarily opportunistic pathogens, decreased in recovered patients (RPs), while the abundance of butyrate-producing organisms increased in these patients. Moreover, these differences were still present for some organisms at 12 months after recovery, indicating the need for long-term monitoring of COVID-19 patients after virus clearance