Control of a single phase unity power factor rectifier

This report presents the implementations and control of single-phase unity power factor rectifier. The implemented rectifier is a bi-directional boost rectifier. An adaptive controller is installed to regulate the output voltage and to achieve a unity power factor and also in the case of loading. The experimental results are provided to show the controller capability in correcting the power factor to unity and regulating output voltage.Bachelor of Engineerin

Experimental observation of effect of the wall curvature of capillary tube on colloidal crystallization inside the tube

We investigated the crystallization process of colloidal suspensions in capillary tubes with different curvature radius. The evolution of structural parameters during crystallization such as crystallinity, average crystallites size and number of crystallites were determined by the reflection spectrum combined with a specially developed data-handling method. We found that the wall curvature has a significant effect on fluctuation, thus the stability of the structural parameters during crystallization process, and with the decrease of the curvature, this effect quickly weakens. In addition, our experiments showed the concentration has lithe influence on crystal stability

Research on Optimization of the Thermal Performance of Composite Rammed Earth Construction

Rammed earth (RE) is a low-tech recyclable building material with good heat storage and moisture absorption performance that can better maintain the stability of the indoor thermal environment and improve indoor comfort. With innovations in and the development of new technology, the field of rammed earth construction technology is gradually expanding. However, deficiencies in the thermal insulation of traditional rammed earth structures make it impossible for them to meet China’s building energy codes in cold regions. This study constructs a comprehensive evaluation index of the thermal performance of rammed earth walls that is based on the heat transfer mechanism, optimizing the thickness of the boundary conditions of the building interior’s design temperature, as well as the energy demand and economic efficiency. This research also offers a new design for the thermal insulation of rammed earth construction by combining the building energy savings design code with WUFI Pro software. This study demonstrates that the optimum thickness of rammed earth construction in Beijing is about 360 mm, the thickness of extruded polystyrene board (XPS) is 50 mm (for public buildings) and 70 mm (for residential buildings), and the structural form of external insulation offers the highest performance benefit. In addition, this work also evaluates the risk of condensation inside composite rammed earth construction, finding that there is a risk of condensation on the exterior side of the wall and at the interface between the insulation panels and rammed earth wall, thus requiring an additional moisture-proof layer. In this study, thermal mass and insulation are fully considered and a design strategy for rammed earth construction given quantitatively, providing a theoretical basis for the application of rammed earth materials in cold regions

Research on Optimization of the Thermal Performance of Composite Rammed Earth Construction

Rammed earth (RE) is a low-tech recyclable building material with good heat storage and moisture absorption performance that can better maintain the stability of the indoor thermal environment and improve indoor comfort. With innovations in and the development of new technology, the field of rammed earth construction technology is gradually expanding. However, deficiencies in the thermal insulation of traditional rammed earth structures make it impossible for them to meet China’s building energy codes in cold regions. This study constructs a comprehensive evaluation index of the thermal performance of rammed earth walls that is based on the heat transfer mechanism, optimizing the thickness of the boundary conditions of the building interior’s design temperature, as well as the energy demand and economic efficiency. This research also offers a new design for the thermal insulation of rammed earth construction by combining the building energy savings design code with WUFI Pro software. This study demonstrates that the optimum thickness of rammed earth construction in Beijing is about 360 mm, the thickness of extruded polystyrene board (XPS) is 50 mm (for public buildings) and 70 mm (for residential buildings), and the structural form of external insulation offers the highest performance benefit. In addition, this work also evaluates the risk of condensation inside composite rammed earth construction, finding that there is a risk of condensation on the exterior side of the wall and at the interface between the insulation panels and rammed earth wall, thus requiring an additional moisture-proof layer. In this study, thermal mass and insulation are fully considered and a design strategy for rammed earth construction given quantitatively, providing a theoretical basis for the application of rammed earth materials in cold regions

Correction: FoxO3a Modulates Hypoxia Stress Induced Oxidative Stress and Apoptosis in Cardiac Microvascular Endothelial Cells.

[This corrects the article DOI: 10.1371/journal.pone.0080342.]

Preparation and environmental toxicity of non-sintered ceramsite using coal gasification coarse slag

In this study, non-sintered ceramsite was prepared using coal gasification coarse slag obtained from a methanol plant. The basic performance and heavy metal leaching toxicity were analyzed. The results showed that seven out of nine non-sintered ceramsite groups were in accordance with the national standard of compressive strength (5 MPa), while only three groups met the national standard of water absorption index of less than 22%. The heavy metal concentrations in these three groups were found to be lower than that specified in National Class IV of surface water environment standards. The concentration of Cr was found to be 16.45 μg/L, which represents only 1% of the IV standard. The optimum mixing ratio, which showed high compressive strength (6.76 MPa) and low water absorption (20.12%), was found to be 73% coal gasification coarse slag, 15% cement, and 12% quartz sand. The characterization using Fourier transform infrared spectroscopy showed that the formation of gelatin in ceramsite enhances the performance of the ceramsite base and increases the immobilization of heavy metal. The study proved that the preparation of non-sintered ceramsite using coal gasification coarse slag reduces its environmental risk and achieves efficient utilization of the slag. Therefore, it can be concluded that it is a feasible and environmental friendly method for the disposal of coal slag

FoxO3a Modulates Hypoxia Stress Induced Oxidative Stress and Apoptosis in Cardiac Microvascular Endothelial Cells

<div><p>Cardiac microvascular endothelial cells (CMECs) dysfunction induced by hypoxia is an important pathophysiological event in myocardium ischemic injury, whereas, the underlying mechanism is not fully clarified. FoxO transcription factors regulate target genes involved in apoptosis and cellular reactive oxygen species (ROS) production. Therefore, the present study was designed to elucidate the potential role of FoxOs on the hypoxia-induced ROS formation and apoptosis in CMECs. Exposure to low oxygen tension stimulated ROS accumulation and increased apoptosis in CMECs within 6–24 h. Hypoxia also significantly increased the expressions of HIF-1α and FoxO3a. However, hypoxia decreased the phosphorylation of Akt and FoxO3a, correlated with increased nuclear accumulation. Conversely, the expression of FoxO1 was not significantly altered by hypoxia. After inhibition of HIF-1α by siRNA, we observed that hypoxia-induced ROS accumulation and apoptosis of CMECs were decreased. Meanwhile, knockdown of HIF-1α also inhibited hypoxia induced FoxO3a expression in CMECs, but did not affect FoxO1 expression. Furthermore, hypoxia-induced ROS formation and apoptosis in CMECs were correlated with the disturbance of Bcl-2 family proteins, which were abolished by FoxO3a silencing with siRNA. In conclusion, our data provide evidence that FoxO3a leads to ROS accumulation in CMECs, and in parallel, induces the disturbance of Bcl-2 family proteins which results in apoptosis.</p></div

Multiplex, variant-tolerant, real-time RT-LAMP for SARS-CoV-2 detection using HFman probe

Viral evolution impacts diagnostic test performance through the emergence of variants with sequences affecting the efficiency of primer binding. Such variants that evade detection by nucleic acid-based tests are subject to selective pressure, enabling them to spread more efficiently. Here, we report a variant-tolerant diagnostic test for SARS-CoV-2 using a loop-mediated isothermal nucleic acid-based amplification (LAMP) assay containing high-fidelity DNA polymerase and a high-fidelity DNA polymerase-medicated probe (HFman probe). In addition to demonstrating a high tolerance to variable SARS-CoV-2 viral sequences, the mechanism also overcomes frequently observed limitations of LAMP assays arising from non-specific amplification within multiplexed reactions performed in a single “pot”. Results showed excellent clinical performance (sensitivity 94.5%, specificity 100%, n = 190) when compared directly to a commercial gold standard reverse transcription quantitative polymerase chain reaction assay for the extracted RNA from nasopharyngeal samples and the capability of detecting a wide range of sequences containing at least alpha and delta variants. To further validate the test with no sample processing, directly from nasopharyngeal swabs, we also detected SARS-CoV-2 in positive clinical samples (n = 49), opening up the possibility for the assay’s use in decentralized testing

Proposed scheme for the mechanism that regulates hypoxia-induced injury in CMECs.

<p>Hypoxia stress increases HIF-1α expression, which subsequently promotes the transcription of FoxO3a and directly stimulates ROS formation. Meanwhile, hypoxia also inhibits the activation of Akt which results in decreased phosphorylation and degradation of FoxO3a. Thus, hypoxia increases FoxO3a translocation to nucleus and the transcription activities, which results in the elevated ROS accumulation and Bcl-2 protein family disturbance. In parallel, increased ROS formation also promotes the activity of FoxO3a which increases the pro-apoptotic proteins Bim and Bax and decreased the anti-apoptotic proteins Bcl-xL and Bcl-2. The disturbance of anti-apoptosis proteins and pro-apoptosis proteins induced by oxidative stress subsequently triggers the apoptosis of CMECs. Accordingly, FoxO3a plays a central role in hypoxia-induced ROS formation and apoptosis in CMECs.</p