Development of Single Nucleotide Polymorphism Markers for the Wheat Curl Mite Resistance Gene Cmc4

Wheat curl mite (Aceria tosichella Keifer) is an important wheat (Triticum aestivum L. em. Thell.) pest in many wheat-growing regions worldwide. Mite feeding damage not only directly affects wheat yield, but A. tosichella also transmits Wheat streak mosaic virus (WSMV). Wheat resistance to A. tosichella, therefore, helps control WSMV. OK05312 (PI 670019) is an advanced breeding line released from Oklahoma that shows a high level of A. tosichella resistance. To map the gene(s) conditioning wheat resistance to A. tosichella in OK05312, a genetic linkage map was constructed using single nucleotide polymorphism (SNP) markers derived from genotyping-by-sequencing (GBS) and a population of 186 recombinant inbred lines (RILs) from the cross ‘Jerry’ (PI 632433)/OK05312. Seedlings of both parents and the RIL population were infested by A. tosichella Biotype 1 in greenhouse experiments. One major quantitative trait locus was identified on the short arm of chromosome 6D, which corresponds to the previously reported gene Cmc4 for A. tosichella resistance. This gene explained up to 71% of the phenotypic variation and was delimited in a 1.7-Mb (?3.3-cM) region by SNPs 370SNP7523 and 370SNP1639. We successfully converted 12 GBS-SNPs into Kompetitive allele specific polymerase chain reaction (KASP) markers. Two of them tightly linked to Cmc4 were validated to be highly diagnostic in a US winter wheat population and can be used for marker-assisted breeding for incorporation of Cmc4 into new wheat cultivars

Tone Mapping Method Based on the Least Squares Method

Tone mapping is used to compress the dynamic range of image data without distortion. To compress the dynamic range of HDR images and prevent halo artifacts, a tone mapping method is proposed based on the least squares method. Our method first uses weights for the estimation of the illumination, and the image detail layer is obtained by the Retinex model. Then, a global tone mapping function with the parameter is used to compress the dynamic range, and the parameter is obtained by fitting the function to the histogram equalization. Finally, the detail layer and the illumination layer are fused to obtain the LDR image. The experimental results show that the proposed method can efficiently restore real-world scene information while preventing halo artifacts. Therefore, tone mapping quality index and mean Weber contrast of the tone-mapped image are 8% and 12% higher than the closest competition tone mapping method

Tone Mapping Method Based on the Least Squares Method

Tone mapping is used to compress the dynamic range of image data without distortion. To compress the dynamic range of HDR images and prevent halo artifacts, a tone mapping method is proposed based on the least squares method. Our method first uses weights for the estimation of the illumination, and the image detail layer is obtained by the Retinex model. Then, a global tone mapping function with the parameter is used to compress the dynamic range, and the parameter is obtained by fitting the function to the histogram equalization. Finally, the detail layer and the illumination layer are fused to obtain the LDR image. The experimental results show that the proposed method can efficiently restore real-world scene information while preventing halo artifacts. Therefore, tone mapping quality index and mean Weber contrast of the tone-mapped image are 8% and 12% higher than the closest competition tone mapping method

Three-Stage Tone Mapping Algorithm

In this paper, a tone mapping algorithm is presented to map real-world luminance into displayed luminance. Our purpose is to reveal the local contrast of real-world scenes on a conventional monitor. Around this point, we propose a three-stage algorithm to visualize high dynamic range images. All pixels of high dynamic range images are classified into three groups. For the first stage, we introduce piecewise linear mapping as the global tone mapping operator to map the luminance of the first group, which provides overall impressions of luminance. For the second stage, the luminance of the second group is determined by the weighted average of its neighborhood pixels, which are derived from the first group’s pixels. For the third stage, the luminance of the third group is determined by the weighted average of its neighborhood pixels, which are derived from the second group’s pixels. Experimental results on several real-world images and the TMQI database show that our algorithm can improve the visibility of real-world scenes with about 12% and 9% higher scores of mean opinion score and tone-mapped image quality index than the closest competitive tone mapping methods. Compared to the existing tone mapping methods, our algorithm produces visually compelling results without halo artifacts and loss of detail

Customer Complaint Avoidance: A Randomized Field Experiment of Platform Governance Based on Value Co-Creation and Appropriation

From the theoretical perspectives of value co-creation and value appropriation in platform governance, this study explores how a platform can design motivation mechanisms to induce the proactive efforts of providers to reduce customer complaints. We conducted a field experiment on a major Chinese peer-to-peer real estate platform offering long-term rental properties. Specifically, when a renter (customer) complaint about a host (provider) occurs, we sent reminder messages to other unaffected hosts in the same neighborhoods (as the affected host) and urged them to proactively prevent similar complaints. The reminder messages varied in terms of their emphasis of the roles of different stakeholders in platform-based value co-creation (provider-emphasized vs. customer-emphasized) and how they explicated different value appropriation mechanisms (competition-based vs. cooperation-based). Results show that compared to the control message, customer-emphasized messages effectively motivated providers’ proactive efforts to reduce customer complaints. Contrastingly, provider-emphasized messages led to the undesirable outcome of increasing customer complaints, possibly due to providers’ shirking behavior. We also found that a competition-based value appropriation mechanism strengthened the motivating effect, whereas a cooperation-based mechanism undermined the motivating effect. The study provides important theoretical and practical implications for platforms on the design of effective governance mechanisms

Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is one of the most devastating diseases of common wheat (Triticum aestivum L.). The wheat line 92145E8-9 is immune to Bgt isolate E09. Genetic analysis reveals that the powdery mildew resistance in 92145E8-9 is controlled by a single dominant gene, temporarily designated Ml92145E8-9. Bulked-segregant analysis (BSA) with simple sequence repeat (SSR) markers indicates that Ml92145E8-9 is located on chromosome 2AL. According to the reactions of 92145E8-9, VPM1 (Pm4b carrier), and Lankao 906 (PmLK906 carrier) to 14 Bgt isolates, the resistance spectrum of 92145E8-9 differs from those of Pm4b and PmLK906, both of which were previously localized to 2AL. To test the allelism among Ml92145E8-9, Pm4b and PmLK906, two F2 populations of 92145E8-9 × VPM1 (Pm4b) and 92145E8-9 × Lankao 906 (PmLK906) were developed in this study. Screening of 784 F2 progeny of 92145E8-9 × VPM1 and 973 F2 progeny of 92145E8-9 × Lankao 906 for Bgt isolate E09 identified 37 and 19 susceptible plants, respectively. These findings indicated that Ml92145E8-9 is non-allelic to either Pm4b or PmLK906. Thus, Ml92145E8-9 is likely to be a new powdery mildew resistance gene on 2AL. New polymorphic markers were developed based on the collinearity of genomic regions of Ml92145E8-9 with the reference sequences of the International Wheat Genome Sequencing Consortium (IWGSC). Ml92145E8-9 was mapped to a 3.6 cM interval flanked by molecular markers Xsdauk13 and Xsdauk682. This study also developed five powdery mildew-resistant wheat lines (SDAU3561, SDAU3562, SDAU4173, SDAU4174, and SDAU4175) using flanking marker-aided selection. The markers closely linked to Ml92145E8-9 would be useful in marker-assisted selection for wheat powdery mildew resistance breeding. Keywords: Marker-assisted selection, Ml92145E8-9, Powdery mildew, Triticum aestivum L

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Fusarium head blight (FHB) is a fungal disease that devastates global wheat production, with losses of billions of dollars annually. Unlike foliar diseases, FHB occurs directly on wheat spikes (inflorescences). The infection lowers grain yield and also causes the grain to be contaminated by mycotoxins produced by the Fusarium pathogen, thus imposing health threats to humans and livestock. Although plant breeders have improved wheat resistance to FHB, the lack of wheat strains with stable FHB resistance has limited progress

Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

Fusarium head blight (FHB), a fungal disease caused by Fusarium species that produce food toxins, currently devastates wheat production worldwide, yet few resistance resources have been discovered in wheat germplasm. Here, we cloned the FHB resistance gene Fhb7 by assembling the genome of Thinopyrum elongatum, a species used in wheat distant hybridization breeding. Fhb7 encodes a glutathione S-transferase (GST) and confers broad resistance to Fusarium species by detoxifying trichothecenes through de-epoxidation. Fhb7 GST homologs are absent in plants, and our evidence supports that Th. elongatum has gained Fhb7 through horizontal gene transfer (HGT) from an endophytic Epichloë species. Fhb7 introgressions in wheat confers resistance to both FHB and crown rot in diverse wheat backgrounds without yield penalty, providing a solution for Fusarium resistance breeding