Analysis of Simulated and Measured Indoor Channels for mm-Wave Beamforming Applications

Ray tracing- (RT-) assisted beamforming, where beams are directly steered to dominant paths tracked by ray tracing simulations, is a promising beamforming strategy, since it avoids the time-consuming exhaustive beam searching adopted in conventional beam steering strategies. The performance of RT-assisted beamforming depends directly on how accurate the spatial profiles of the radio environment can be predicted by the RT simulation. In this paper, we investigate how ray tracing-assisted beamforming performs in both poorly furnished and richly furnished indoor environments. Single-user beamforming performance was investigated using both single beam and multiple beams, with two different power allocation schemes applied to multibeamforming. Channel measurements were performed at 28–30 GHz using a vector network analyzer equipped with a biconical antenna as the transmit antenna and a rotated horn antenna as the receive antenna. 3D ray tracing simulations were carried out in the same replicated propagation environments. Based on measurement and ray tracing simulation data, it is shown that RT-assisted beamforming performs well both for single and multibeamforming in these two representative indoor propagation environments

The Ubiquitin/Proteasome System Mediates Entry and Endosomal Trafficking of Kaposi's Sarcoma-Associated Herpesvirus in Endothelial Cells

Ubiquitination, a post-translational modification, mediates diverse cellular functions including endocytic transport of molecules. Kaposi's sarcoma-associated herpesvirus (KSHV), an enveloped herpesvirus, enters endothelial cells primarily through clathrin-mediated endocytosis. Whether ubiquitination and proteasome activity regulates KSHV entry and endocytosis remains unknown. We showed that inhibition of proteasome activity reduced KSHV entry into endothelial cells and intracellular trafficking to nuclei, thus preventing KSHV infection of the cells. Three-dimensional (3-D) analyses revealed accumulation of KSHV particles in a cytoplasmic compartment identified as EEA1+ endosomal vesicles upon proteasome inhibition. KSHV particles are colocalized with ubiquitin-binding proteins epsin and eps15. Furthermore, ubiquitination mediates internalization of both KSHV and one of its receptors integrin β1. KSHV particles are colocalized with activated forms of the E3 ligase c-Cbl. Knock-down of c-Cbl or inhibition of its phosphorylation reduced viral entry and intracellular trafficking, resulting in decreased KSHV infectivity. These results demonstrate that ubiquitination mediates internalization of both KSHV and one of its cognate receptors integrin β1, and identify c-Cbl as a potential E3 ligase that facilitates this process