Apoptosis induced by membrane damage in human lymphocytes; effects of arachidonic acid and its photoproducts.

The effect of arachidonic acid (AA) combined with UVA irradiation was studied in a model system mimicking phototherapy PUVA (psoralen+UVA) ex vivo in vitro. The contribution of damage to the plasma membrane by PUVA was tested on human lymphocytes derived from healthy donors. The effect of arachidonic acid (AA) combined with UVA irradiation was compared with that of a psoralen photoadduct to AA added to the culture. The adduct, obtained photochemically and purified, was characterized by NMR and MS spectrometry as a cycloadduct of psoralen to the vinylene bond of the acid (AAPSO). The reactions of cultured cells, manifested 20 h after treatment by changes in apoptosis and mitochondrial depolarization, were monitored by flow cytometry by tagging lymphocytes with appropriate fluorescent probes. Treatment of lymphocyte suspension within AA doses from 40 to 100 μM gradually induced a shift from Anx-V+ (single positive cells) to late apoptotic, Anx-V+PI+ (double positive cells) in a dose dependent manner. The adduct, AAPSO, induced apoptotic changes at a concentration 2-3 times higher than free AA. Combination of psoralen (1 μM ) or arachidonic acid (20-120 μM) with UVA irradiation (2-6 J/cm2) accelerated the plasma membrane changes in a synergic way. Preliminary studies indicated that changes in the transmembrane potential of mitochondria paralleled the apoptosis when cells were treated by AA alone. Our findings showed that UVA radiation of lymphocytes in the presence of arachidonic acid, as in the presence of psoralen, enhanced apoptosis of cells in a synergic manner. Thus, PUVA-induced apoptosis may proceed in part by a still undefined signaling pathway(s) triggered in lymphocyte membranes

Sustainable Green Processing of Grape Pomace Using Micellar Extraction for the Production of Value-Added Hygiene Cosmetics

This study sought to evaluate the possibility of using grape pomace, a waste material from wine production, for the preparation of cosmetic components. Following the existing clear research trend related to improving the safety of cleansing cosmetics, an attempt was made to determine the possibility of preparing model shower gels based on grape pomace extract. A new method for producing cosmetic components named loan chemical extraction (LCE) was developed and is described for the first time in this paper. In the LCE method, an extraction medium consisting only of the components from the final product was used. Thus, there were no additional substances in the cosmetics developed, and the formulation was significantly enriched with compounds isolated from grape pomace. Samples of the model shower gels produced were evaluated in terms of their basic parameters related to functionality (e.g., foaming properties, rheological characteristics, color) and their effect on the skin. The results obtained showed that the extracts based on waste grape pomace contained a number of valuable cosmetic compounds (e.g., organic acids, phenolic compounds, amino acids and sugars), and the model products basis on them provided colorful and safe natural cosmetics

Sustainable Green Processing of Grape Pomace Using Micellar Extraction for the Production of Value-Added Hygiene Cosmetics

This study sought to evaluate the possibility of using grape pomace, a waste material from wine production, for the preparation of cosmetic components. Following the existing clear research trend related to improving the safety of cleansing cosmetics, an attempt was made to determine the possibility of preparing model shower gels based on grape pomace extract. A new method for producing cosmetic components named loan chemical extraction (LCE) was developed and is described for the first time in this paper. In the LCE method, an extraction medium consisting only of the components from the final product was used. Thus, there were no additional substances in the cosmetics developed, and the formulation was significantly enriched with compounds isolated from grape pomace. Samples of the model shower gels produced were evaluated in terms of their basic parameters related to functionality (e.g., foaming properties, rheological characteristics, color) and their effect on the skin. The results obtained showed that the extracts based on waste grape pomace contained a number of valuable cosmetic compounds (e.g., organic acids, phenolic compounds, amino acids and sugars), and the model products basis on them provided colorful and safe natural cosmetics

Micellar and Solvent Loan Chemical Extraction as a Tool for the Development of Natural Skin Care Cosmetics Containing Substances Isolated from Grapevine Buds

The present study aimed to evaluate the possibility of using micellar and solvent loan chemical extraction (LCE) to isolate valuable components from grapevine leaf buds, subsequently utilizing them to prepare functional and safe-to-use cosmetic preparations, specifically facial serums. An aqueous solution of polyglyceryl-4 laurate/sebacate and polyglyceryl-6 caprylate/caprate was employed for a micellar LCE, while an aqueous solution of 1,3-propanediol was used for a solvent LCE. Importantly, the extraction medium was exclusively comprised of components from the designed final cosmetic product. Consequently, no additional substances were present in the cosmetics developed, and the formulation was notably enhanced by compounds extracted from grapevine buds. The antioxidant properties and compound characterization of the obtained micellar (SurfE) and solvent (SolvE) extracts based on grapevine buds were tested and compared. UPLC-MS/MS results indicated that the extracts were rich in phenolic and flavonoid compounds, exhibiting antioxidant activity as measured using the DPPH and ABTS scavenging ability. The extracts were used to prepare model facial serums, which underwent evaluation based on fundamental functionality-related parameters (e.g., rheological characteristics and color) and their impact on the skin through cytotoxicity assessment. The results demonstrated that facial serums with extracts based on grapevine buds provided safe, natural cosmetics