IGL-1 solution reduces endoplasmic reticulum stress and apoptosis in rat liver transplantation

Injury due to cold ischemia reperfusion (I/R) is a major cause of primary graft non-function following liver transplantation. We postulated that I/R-induced cellular damage during liver transplantation might affect the secretory pathway, particularly at the endoplasmic reticulum (ER). We examined the involvement of ER stress in organ preservation, and compared cold storage in University of Wisconsin (UW) solution and in Institute Georges Lopez-1 (IGL-1) solution. In one group of rats, livers were preserved in UW solution for 8 h at 4 °C, and then orthotopic liver transplantation was performed according to Kamada's cuff technique. In another group, livers were preserved in IGL-1 solution. The effect of each preservation solution on the induction of ER stress, hepatic injury, mitochondrial damage and cell death was evaluated. As expected, we found increased ER stress after liver transplantation. IGL-1 solution significantly attenuated ER damage by reducing the activation of three pathways of unfolded protein response and their effector molecules caspase-12, C/EBP homologous protein-10, X-box-binding protein 1, tumor necrosis factor-associated factor 2 and eukaryotic translation initiation factor 2. This attenuation of ER stress was associated with a reduction in hepatic injury and cell death. Our results show that IGL-1 solution may be a useful means to circumvent excessive ER stress reactions associated with liver transplantation, and may optimize graft quality

Processament d'imatges de satèl·lit amb QGIS = Procesamiento de imágenes satélite con QGIS

Taller a càrrec de Ferran Orduña, Carla Garcia (SIGTE - Universitat de Girona), que té com a objectiu aprendre a processar imatges de satèl·lit Landsat i/o Sentinel utilitzant el software QGIS. S'exploren les millors combinacions de bandes a l'hora d'interpretar diferents usos del sòl i es treballa amb el complement SCP (semi-automatic classification plugin) mitjançant el qual es poden realitzar classificacions supervisades per obtenir una capa d'usos del sòl. Es veu com millorar el resultat obtingut en la classificació d'usos del sòl mitjançant l'ús d'algunes tècniques com la introducció de l'índex NDVI o la reducció de l'efecte dels núvol