Additional file 1: of Gene expression analysis in asthma using a targeted multiplex array

Supplementary Methods – Methods describing selection of house keeping genes and immunohistochemical staining procedure. Supplementary Tables – Tables containing clinical demographics for subjects, average counts, fold change, and p-value for all genes studied, and all differentially co-expressed genes. Supplementary Figures and Legends – Figures showing sample immunohistochemical staining for proteins of significantly altered genes, co-expression plots. (DOCX 35 kb

Volcano plot of gene differentially expressed between statin users and non-users.

<p>The plot shows the log fold-change on the X-axis versus the unadjusted P values (on the–log10 scale) on the Y-axis. The blue and red dots represent genes that showed fold change in either direction that is greater than 0.05 and have unadjusted P value <0.01. Genes that had an FDR-adjusted P value less than 0.05 for differential expression are annotated on the graph.</p

GO biological processes’ enrichment among statin differentially expressed genes.

<p>Adjusted P value: The Benjamini Hochberg Adjusted P value. No. of genes: number of genes in this pathway present among the 18 genes tested.</p><p>GO biological processes’ enrichment among statin differentially expressed genes.</p

Study participants’ demographics.

<p>Continuous variables are shown as mean+ standard error of man (SEM). Difference in demographics between statin users and non-users was calculated using Student’s t-test for continuous variables and Chi-square test for categorical variables</p><p>Study participants’ demographics.</p

Statins’ differentially expressed genes at 10% FDR.

<p>LogFC: log 2 fold change. Adj.p.value: Benjamini Hochberg Adjusted P value</p><p>Statins’ differentially expressed genes at 10% FDR.</p