11 research outputs found
Image_1_A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets.JPEG
Immunoglobulin A (IgA) of sows is critically important for assessing piglets’ protective capacity against porcine epidemic diarrhea virus (PEDV). Here, we report a therapeutic chimeric anti-PEDV IgG/IgA expressed by Chinese hamster ovary (CHO) cells for oral treatment of PED. The chimeric anti-PEDV IgG/IgA was produced by the CHO cell lines, in which the heavy chain was constructed by combining the VH, Cγ1 and hinge regions of PEDV IgG mAb 8A3, and the Cα2 and Cα3 domains of a Mus musculus immunoglobulin alpha chain. The chimeric anti-PEDV IgG/IgA could neutralize the strains of CV777 (G1), P014 (G2) and HN1303 (G2) in vitro effectively, showing broad-spectrum neutralization activity. The in vivo challenge experiments demonstrated that chimeric anti-PEDV IgG/IgA (9C4) produced in the CHO cell supernatant could alleviate clinical diarrhea symptoms of the PEDV infection in piglets. In general, our study showed that chimeric anti-PEDV IgG/IgA produced from CHO cell line supernatants effectively alleviates PEDV infection in piglets, which also gives the foundation for the construction of fully functional secretory IgA by the J chain introduction to maximize the antibody therapeutic effect.</p
Additional file 16: Table S10. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
Feed ingredients. (XLSX 10 kb
Additional file 8: Figure S3. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
A linear discriminant analysis effect size cladogram representing the significantly different taxa in a tree-like structure. (DOC 249 kb
Additional file 12: Table S8. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
Time dependent alteration of bile acids and microbes in control and HFD groups. (XLSX 33 kb
Additional file 11: Figure S4. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
The trajectories of bile acids and microbiome alteration along 56 days in control and HFD groups. (DOC 167 kb
Additional file 3: Table S2. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
Metabolome profile of caecal content in control and HFD groups at 8 weeks. (XLSX 39 kb
Additional file 5: Table S4. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
Concentrations of bile acids in caecal content in each group. (XLSX 48 kb
Additional file 6: Figure S2. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
The microbial community diversity in control and HFD groups shown by Simpson reciprocal index. (DOC 54 kb
Additional file 4: Table S3. of Bile acid is a significant host factor shaping the gut microbiome of diet-induced obese mice
Correlation of metabolite types and microbial phyla. (XLSX 16 kb