Effects of knocking down Bcl-3 on UV stress-induced suppression of CTCF.

<p>(A) Time-dependent effect of knocking down Bcl-3 on UV stress-induced suppression of CTCF detected by Western analysis. (B) Time-dependent effect of knocking down Bcl-3 on UV stress-induced inhibition of CTCF mRNA expression by RT-PCR. (C) Quantitative detection of UV stress-induced CTCF mRNA suppression in Bcl-3 knocking down cells by real time PCR. (D) Effect of knocking down Bcl-3 on UV stress-induced suppression of CTCF promoter activity. Data were plotted as Mean±SE and statistical significance was determined at <i>p</i><0.05 (n = 3 to 6).</p

UV stress-induced activation and interaction of Bcl-3 and p50.

<p>(A) Immuno-coprecipitation of Bcl-3 and p50 pulled down by anti-Bcl-3 antibodies. (B) Immuno-coprecipitation of Bcl-3 and p50 pulled down by anti-p50 antibodies. (C) Nuclear immuno-colocalization of Bcl-3 and p50 in UV stress-induced HCE cells. Arrows indicate activated p50, Bcl-3 and p50+Bcl-3 localized in the nucleus. (D) Statistical significance of immuno-colocalized nuclear Bcl-3 and p50 in UV stress-induced HCE cells. Bcl-3 and p50 in control, EGF stimulated and UV stress-induced HCE cells were detected by immunostaining experiments with specific antibodies against Bcl-3 and p50. Cell nuclei were detected by DAPI staining. Arrows are indicating immune activities that by were imaged by using a Nikon fluorescent microscope at 40×, and data were analyzed by Nikon software. Symbol “*” indicates significant differences (<i>p</i><0.05, n = 26).</p

Interaction of Bcl-3 and p50 with CTCF promoter.

<p>(A) EGF- and UV stress-induced interactions of Bcl-3 and p50 with CTCF promoter detected by ChIP assays. (B) Analysis of interactions between Bcl-3/p50 and CTCF promoter. (C) Effect of over-expression of Bcl-3 and p50 on activities of wildtype and deletion mutant of CTCF promoter. (D) Dose-response relationship between over-expression of Bcl-3 and suppression of CTCF expression. HCE cells were transfected with full-length cDNAs encoding Bcl-3 (a generous gift from Dr. Shin-Ichiro Takahashi at the University of Tokyo) and p50, CTCF reporter and the mutant CTCF reporter with κB site-deletion by lipofection. Symbol “*” indicates significant differences between control and transfected cells (<i>p</i><0.05, n = 4).</p

Effects of EGF- and UV stress-induced activation of NF-κB pathways.

<p>(A) Time course EGF-induced phosphorylation of IκBα. (B) Time course EGF-induced degradation of IκBα. (C) Effect of UV stress on phosphorylation of IκBα. (D) Effect of UV stress on degradation of IκBα. (E) Effect of EGF stimulation on nuclear activities of p50, p65 and Bcl-3. (F) UV stress-induced nuclear activities of p50, p65 and Bcl-3. HCE cells were synchronized by serum-depletion for 24 h. Total and nuclear proteins were extracted following stimulation at indicated time points and detected by Western analysis. Symbol “*” indicates significant differences between control and induced HCE cells (<i>p</i><0.05, n = 3).</p

Effect of EGF and UV stress on CTCF activity.

<p>(A) Time course of UV stress-induced changes in Bcl-3 and CTCF activity. (B) Quantitative detection of UV stress-induced effect on CTCF mRNA expression by real time PCR. (C) Effect of EGF on Bcl-3 and CTCF activity following a time course. (D) Quantitative detection of EGF-induced effect on CTCF mRNA expression by real time PCR. Proteins and RNA were isolated from HCE cells at indicated time-point before/after EGF (20 ng/ml) and UV irradiation (42 µJ/cm²), respectively. Symbol “*” indicates significant differences between control and UV stress-induced cells (<i>p</i><0.05, n = 3).</p

Additional file 2 of Dimensions of spiritual well-being in relation to physical and psychological symptoms: a cross-sectional study of advanced cancer patients admitted to a palliative care unit

Additional file 2. Raw data

Additional file 1 of Dimensions of spiritual well-being in relation to physical and psychological symptoms: a cross-sectional study of advanced cancer patients admitted to a palliative care unit

Additional file 1: Table A. Results of regression analyses exploring associations between spiritual well-being and cancer-related symptoms

Number of significant interactions for yield and yield components detected at 0.001 probability by permutation tests of all possible two loci combinations in hybrids evaluated at Handan (S1) and Cangzhou (N1.).

<p>Number of significant interactions for yield and yield components detected at 0.001 probability by permutation tests of all possible two loci combinations in hybrids evaluated at Handan (S1) and Cangzhou (N1.).</p

Correlation between yield and yield components in F_{2: 3} and F_{2: 4} populations.

<p>*, ** Significant at probability of 0.05 and 0.01 respectively.</p><p>Correlation between yield and yield components in F_{2: 3} and F_{2: 4} populations.</p

The best and the worst single heterozygotes in each of the two loci combinations showing significant AD/DA interactions for bolls/plant in F_{2: 3} population.

<p>See footnotes of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143548#pone.0143548.t007" target="_blank">Table 7</a> for explanations</p><p>The best and the worst single heterozygotes in each of the two loci combinations showing significant AD/DA interactions for bolls/plant in F_{2: 3} population.</p