Genome-wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β-catenin paradigm

We would like to thank Ken Cho, Ira Blitz, Kris Vleminckx and Aaron Zorn for discussion and Adam Lynch for comments on the manuscript. The research of the authors is supported by the UK Biotechnology and Biological Sciences Research Council (BB/M001695/1).Peer reviewe

The Buchsbaum property of symbolic powers of Stanley–Reisner ideals of dimension 1

AbstractLet S be a polynomial ring and I be the Stanley–Reisner ideal of a simplicial complex Δ. The purpose of this paper is investigating the Buchsbaum property of S/I(r) when Δ is pure dimension 1. We shall characterize the Buchsbaumness of S/I(r) in terms of the graphical property of Δ. That is closely related to the characterization of the Cohen–Macaulayness of S/I(r) due to the first author and N.V. Trung

Induction of reparative dentin by calcium silicate-based material as direct pulp capping agent

This study was performed to examine whether calcium silicate could induce reparative dentin formation without eliciting any adverse effect in direct pulp capping of premolar teeth. Twenty participants who need extraction of their 4 healthy permanent premolar teeth for orthodontic reasons were included in this study. Following the surgical procedure, the exposed pulp tissue was treated either with calcium silicate or covered with calcium hydroxide paste. On day 3, 7, 14 and 28, the experimental teeth was extracted and examined using light microscopy and histometric analysis to observe the inflammatory changes and the amount of reparative dentin formation. The results showed that in the calcium silicate treated teeth, substantial amounts of dentine-like tissue was formed on day 14 and mostly located on the exposure site. It was also observed in the calcium hydroxide treated teeth but dentin-like tissue located at a distance from the exposure site. The total amount of reparative dentine formed in the calcium silicate-treated teeth was significantly higher (p<0.005) than in the calcium hydroxide-treated specimens. In conclusion that the calcium silicate indices pulpal wound healing and reparative formation in the exposed teeth without affecting the normal function of the remaining pulp

Plasticity of Cells and Ex Vivo Production of Red Blood Cells

The supply of transfusable red blood cells (RBCs) is not sufficient in many countries. If transfusable RBCs could be produced abundantly from certain resources, it would be very useful. Our group has developed a method to produce enucleated RBCs efficiently from hematopoietic stem/progenitor cells present in umbilical cord blood. More recently, it was reported that enucleated RBCs could be abundantly produced from human embryonic stem (ES) cells. The common obstacle for application of these methods is that they require very high cost to produce sufficient number of RBCs that are applicable in the clinic. If erythroid cell lines (immortalized cell lines) able to produce transfusable RBCs ex vivo were established, they would be valuable resources. Our group developed a robust method to obtain immortalized erythroid cell lines able to produce mature RBCs. To the best of our knowledge, this was the first paper to show the feasibility of establishing immortalized erythroid progenitor cell lines able to produce enucleated RBCs ex vivo. This result strongly suggests that immortalized human erythroid progenitor cell lines able to produce mature RBCs ex vivo can also be established