4 research outputs found
Enzymatic Synthesis of Novel Phenol Acid Rutinosides Using Rutinase and Their Antiviral Activity in Vitro
Novel rutinosides of vanillic acid,
sinapic acid, ferulic acid,
and caffeic acid were prepared via a rutinase-catalyzed transglycosylation
reaction. Reaction mixtures containing rutin, phenolic acid, and rutinase
derived from tartary buckwheat (Fagopyrum tataricum) seeds were incubated in 20 mM acetate buffer (pH 5.0) at 40 °C.
The resulting rutinoside of each phenolic acid was purified by HPLC,
and the structure was determined by NMR and FAB-MS analysis. Antiviral
activity was determined using feline calicivirus (FCV) strain F9,
which is a typical norovirus surrogate. It was found that rutinosylation
of the phenolic acids increased their antiviral activity against FCV,
with the sinapic acid rutinoside being the most effective. These results
will contribute to the development of antiviral agents against noroviruses
Discovery-Based Science Education: Functional Genomic Dissection in Drosophila by Undergraduate Researchers
Discovery-Based Science Education: Functional Genomic Dissection in Drosophila by Undergraduate Researcher
Example of the Type of Data Available from the Online Database (http://www.bruinfly.ucla.edu)
<p>Example of the Type of Data Available from the Online Database (<a href="http://www.bruinfly.ucla.edu" target="_blank">http://www.bruinfly.ucla.edu</a>)</p
Representative Pictures from the Laboratory Section of the Course
<p>Representative Pictures from the Laboratory Section of the Course</p