Key upstream regulator networks modulated by arsenic exposure.

<p>The upstream network was generated by IPA and the networks indicate predicted upstream regulators and their downstream target genes presented in the differentially expressed gene set. Up-regulated genes are in red and down-regulated in green. Solid arrow lines represent direct interaction while dotted lines indirect intereaction.</p

Preliminary identification of potential biomarker genes for arsenic exposure.

<p>Selected up-regulated genes by arsenic exposure were examined by RT-qPCR in individual zebrafish (A) and medaka (B) after treatment with arsenic. (A), Fold changes (log2 ratio) of 14 up-regulated genes measured by RT-qPCR. S1-S9, 9 individual zebrafish treated with 15 ppm sodium; 15 ppm, average of the 9 individual fish; 20 ppm, RT-qPCR measurement from the pooled RNA sample used for RNA-SAGE sequencing; SAGE, RNA-SAGE data for comparison (Log2 fold change). The 9 genes displayed dosage-dependent effect between 15 ppm and 20 ppm are indicated with asterisks. Zebrafish gene symbols and names are shown based on NCBI and underlined genes are annotated manually. (B), Average of fold changes (log2 ratio) of four validated medaka genes in 4 individual medaka fish. (C) Comparison of the expression of arsenic biomarker genes in other chemical treatments by hierarchical clustering heatmap. RNA-SAGE data from the current study (Arsenic) were compared with hepatic RNA-SAGE data from zebrafish treated with 5 µg/L 17β-estradiol (E2), 5 µg/L 11-keto testosterone (KT11) or 10 nM 2,3,7,8-tetrachlorodibenzo-<i>p</i>-dioxin (TCDD). The left clustering is based on the 14 genes identified from zebrafish and the right based on the four genes from both zebrafish and medaka.</p

Table_6_Involvement of JMJ15 in the dynamic change of genome-wide H3K4me3 in response to salt stress.XLSX

Post-translational histone modifications play important roles in regulating chromatin structure and transcriptional regulation. Histone 3 lysine 4 trimethylation (H3K4me3) is a prominent histone modification mainly associated with gene activation. Here we showed that a histone demethylase, JMJ15, belonging to KDM5/JARID group, is involved in salt stress response in Arabidopsis thaliana. Jmj15 loss-of-function mutants displayed increased sensitivity to salt stress. Moreover, knockout of JMJ15 impaired the salt responsive gene expression program and affected H3K4me3 levels of many stress-related genes under salt-stressed condition. Importantly, we demonstrated that JMJ15 regulated the expression level of two WRKY transcription factors, WRKY46 and WRKY70, which were negatively involved in abiotic stress tolerance. Furthermore, JMJ15 directly bound to and demethylated H3K4me3 mark in the promoter and coding regions of WRKY46 and WRKY70, thereby repressing these two WRKY gene expression under salt stress. Overall, our study revealed a novel molecular function of the histone demethylase JMJ15 under salt stress in plants.</p

Coliquefaction of coal and polystyrene in supercritical water

<p>The coliquefaction of coal and polystyrene (PS) in supercritical water (SCW) was carried out in a 50-mL batch stainless steel autoclave reactor, and the effects of the polymer ratio by weight (10–40%), reaction temperature (633.5–703.5 K), and reaction time (30–120 min) were investigated. The main products were analyzed qualitatively by Fourier transform infrared spectroscopy and high-performance liquid chromatography. The results show that polystyrene stimulates coal liquefaction as a hydrogen donor, and the synergistic effects during coliquefaction in SCW were confirmed. The conversion reached a maximum of 62.26% after 60 min at 673.5 K. The phase behavior during coliquefaction was observed in a fused silica capillary reactor using a combined microscope and video recorder system.</p

Significantly affected gene ontology terms by arsenic exposure.

<p>Note: Count is number of genes involved. Fold Enrichment is (m/n)/(M/N), where <b>N</b> = all genes in zebrafish, <b>M</b> = all genes belonging to a GO term, <b>n</b> = genes from the differentially expressed gene set, <b>m</b> = genes from the differentially expressed gene set belonging to a GO term.</p

Diseases inferred by IPA based on differentially expressed genes after arsenic exposure.

<p>The bar chart shows the number of arsenic-deregulated genes matched in different disease or disorder categories and only top significant categories (P<0.01) were selected to show. The same gene may be assigned to more than one categories.</p

Enriched KEGG pathways identified by Gene Set Enricher of CTD.

<p>Note: Pathways indicated with asterisks are newly identified in the present study.</p

Comparison of transcriptomic profiles between arsenic-treated and control groups.

<p>(A) Distribution of transcript entries and total transcript counts in both arsenic-treated and control groups. The percentages of accumulated transcript counts or transcript entries are plotted over different transcript abundance categories. (B) Plot of transcript change fold (Y-axis) versus transcript TPM (X-axis) after arsenic exposure. Both axes are in log2 scale and TPM in the X-axis is based on the treatment group.</p

sj-docx-1-jop-10.1177_02698811241248391 – Supplemental material for Mining and analysis of security alert signals of valbenazine based on the Food and Drug Administration Adverse Event Reporting System database

Supplemental material, sj-docx-1-jop-10.1177_02698811241248391 for Mining and analysis of security alert signals of valbenazine based on the Food and Drug Administration Adverse Event Reporting System database by Qi Wang, Kankan Qu, Zhiqiang Du, Yuan Shen, Ying Jiang and Haohao Zhu in Journal of Psychopharmacology</p

The moderating role of COMT and BDNF polymorphisms on transfer effects following multi- and single-domain cognitive training among community-dwelling Shanghainese older adults

Given the increase in research suggesting benefit following cognitive training in older adults, researchers have started to investigate the potential moderating role of genetic polymorphisms on transfer effects. The objective of this study was to evaluate the moderating effect of catechol-O-methyltransferase (COMT) and brain-derived neurotrophic factor (BDNF) polymorphisms on transfer effects following a single-domain or multi-domain training intervention in healthy community-dwelling older adults. A total of 104 men and women living in Shanghai were randomized to a multi-domain or a single-domain cognitive training (SDCT) group. COMT rs4818 SNP and the BDNF rs6265 SNP were analyzed from blood. At pre-intervention, post-intervention and at 6-month follow-up, participants completed the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS), the Color-Word Stroop Test (CWST), the Trails Making Test (TMT) and the Visual Reasoning Test (VRT). COMT was found to moderate immediate memory transfer effects following single-domain training only, with G/- carriers displaying greater benefits than C/C carriers. BDNF was found to moderate attention and inhibition independent of the training, with Met/- carriers displaying better performance than Val/Val carriers. Overall, individualizing training methods with full consideration of genetic polymorphisms may promote the maximization of cognitive training benefits.  </p