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Additional file 18: of CLICK: one-step generation of conditional knockout mice
Table S4. Primer sets used for PCR and sequencing analysis in this study. (XLSX 10 kb
Additional file 18: of CLICK: one-step generation of conditional knockout mice
Table S4. Primer sets used for PCR and sequencing analysis in this study. (XLSX 10 kb
Additional file 1: of CLICK: one-step generation of conditional knockout mice
Figure S1. Schematic representation of the protocol to prepare long single-stranded DNA (lssDNA) using nicking endonucleases. (AI 1147 kb
Additional file 11: of CLICK: one-step generation of conditional knockout mice
Figure S11. Sequences of two gRNAs and lssDNA(+) designed to target exon 2 of the rat Vapb locus. (AI 1013 kb
Additional file 2: of CLICK: one-step generation of conditional knockout mice
Figure S2. Sequences of two gRNAs and a complementary lssDNA(−) designed to target exon 4 of the mouse Serpina3n locus. (AI 1215 kb
Additional file 3: of CLICK: one-step generation of conditional knockout mice
Figure S3. Floxed Serpina3n alleles generated by the microinjection of two gRNA, Cas9 mRNA and lssDNA(−) into B6 mouse zygotes. (AI 1263 kb
Additional file 17: of CLICK: one-step generation of conditional knockout mice
Table S3. Crossing floxed founders with Cre-driver mice. (XLSX 11 kb
Additional file 6: of CLICK: one-step generation of conditional knockout mice
Figure S6. Sequences of two gRNAs and lssDNA(−) designed to target exon 3 of the mouse KIAA1322 locus. (AI 1214 kb
Additional file 9: of CLICK: one-step generation of conditional knockout mice
Figure S9. Sequences of two gRNAs and lssDNA(+) designed to target exon 3 of the mouse Mct4 locus. (AI 1209 kb
Additional file 15: of CLICK: one-step generation of conditional knockout mice
Table S1. Germline transmission of CLICK-mediated mutations in F1 offspring. (XLSX 12 kb