Additional file 3: Figure S2. of Identification of definitive serum biomarkers associated with disease activity in primary Sjögren’s syndrome

Serum levels of five proteins in pSS, sSS, sicca syndrome and HCs. The five proteins were CXCL13, TNF-R2, CD48, BAFF and PD-L2. Primary SS (pSS), n = 58; secondary SS (sSS), n = 6; other sicca syndrome, n = 13; healthy controls (HCs), n = 38. Differences in quantitative variables were analyzed by the Mann-Whitney U test when comparing two groups and by the Kruskal-Wallis test when comparing multiple groups. *P value <0.05, which was considered significant. (TIF 33972 kb

Additional file 2: Figure S1. of Identification of definitive serum biomarkers associated with disease activity in primary Sjögren’s syndrome

Functional annotation of differentially expressed proteins in pSS patient sera. Nodes indicate molecular concepts or set of biologically related genes. Name of each node is indicated in black text on the node. The node size represents the proportion of differentially expressed gene symbols in the concepts (e.g., the “chemokine signaling pathway” and “extracellular region” concepts contain 14 and 58 genes, respectively). Length of lines between nodes represents degree of overlap between symbols. Colored lines indicate strength of functional relationship from strong to weak, as follows: red, yellow, green and gray. Green nodes indicate immune response-related molecular concepts, and red nodes indicate platelet-related molecular concepts. (TIF 9752 kb

Additional file 1: Figure S1. of Enhanced IgG4 production by follicular helper 2 T cells and the involvement of follicular helper 1 T cells in the pathogenesis of IgG4-related disease

Flow cytometric analysis of the percentage of circulating follicular helper T (Tfh) cells and activated Tfh cells. The percentage of Tfh cells (A) and activated Tfh cells (B) from patients with IgG4-related disease (IgG4-RD) (n = 17), primary Sjögren’s syndrome (pSS) (n = 20), multicentric Castleman’s disease (MCD) (n = 5), and healthy controls (HC) (n = 12). *P < 0.05; **P < 0.0001 for analysis using the Kruskal-Wallis test, followed by group-wise comparisons using the Mann-Whitney U test. (TIF 42 kb