Additional file 1: of Sociodemographic, clinical, and psychosocial factors associated with depression among type 2 diabetic outpatients in Black Lion General Specialized Hospital, Addis Ababa, Ethiopia: a cross-sectional study

This is a cleaned comma delimited (*.csv) dataset titled “Patients sociodemographic, clinical, psychosocial, and depressive symptom raw data”. It comprises patients’ sociodemographic, clinical, psychosocial, and depressive symptoms information. All data was anonymized. (CSV 87 kb

Additional file 2: of Sociodemographic, clinical, and psychosocial factors associated with depression among type 2 diabetic outpatients in Black Lion General Specialized Hospital, Addis Ababa, Ethiopia: a cross-sectional study

STROBE Statement. (DOC 89 kb

Additional file 1: Figure S1. of Granzyme B-inhibitor serpina3n induces neuroprotection in vitro and in vivo

A representative section from the lumbar part of the spinal cord. The regions under the blue-lined rectangular boxes show the areas where CD4+ T cells and SMI32-positive axons were quantified and analyzed. (PPTX 6495 kb

List of primers and their sequences or catalogue numbers used in the study.

<p>List of primers and their sequences or catalogue numbers used in the study.</p

Microarray-based gene analysis.

<p>The graphs show cluster analysis of Row Z-score data of (A) pluripotent/embryonic, (B) endothelial, as well as (C) neuronal and glial genes expressed by HUVECs, HiPSCs, HiPSC-derived neurons (HiPSC-Ns), HFNs and samples obtained from previous publications as described in the Materials and Methods (hESCs, hESC-NSCs, hESC-SCNTs). Data are average values of 3 independent experiments.</p

Characterization of HUVEC-derived iPSCs (HiPSCs).

<p>Gene expression was determined after 5 passages of the colonies. RT-PCR results (mRNA levels) showing endogenous embryonic (A) and endothelial (B) specific genes expressed by HUVECs and HiPSCs. Values in Figure B represent fold reduction compared to HUVECs. Data are average values of 4 HiPSC lines (N = at least 3 replicates; P<0.001). Non-detectable genes are denoted as n.d. At protein levels using immune-staining, representative micrographs show expression of embryonic markers-SSEA-4 and Oct 3/4 (C and D respectively), DAPI (E) and lack of expression of endothelial markers- PECAM (CD31) and VE-cadherin (F and G respectively) in HiPSC colonies. H is DAPI co-staining of panels F and G. Scale bar: 200 μm.</p

Morphological comparison of human fetal neurons (HFNs) and HiPSC-derived neurons.

<p>Neurons were immuno-stained for MAP-2 and their morphology was manually evaluated. Micrographs showing MAP-2 positive (A) HFNs and (B) HiPSC-derived neurons. The graphs compare average (C) size of cell bodies, (D) number of neurites/cell, and (E) neurite length/cell of HFNs and HiPSC-derived neurons. P< 0.001; Scale bar: 100 μm. The experiment was repeated 3 times, and at least 50 neuronal cells were evaluated in each group.</p

Susceptibility of HiPSC-derived neurons to inflammatory cells.

<p>MAP-2 immuno-stained micrographs showing that HiPSC-derived neurons were either (A) treated with neuronal media, (B) co-cultured with unactivated T cells or (C) co-cultured with activated T cells. (D) shows quantification of MAP-2 positive surviving neurons.</p

Feeder-layer independent induced pluripotent stem cells generation from HUVECs (HiPSCs).

<p>(A) HUVECs were transduced with lentiviral vectors. Within 4 days (B) and a week (C) the endothelial cells were reduced in density but formed aggregates. (D) On day 16, immature colonies emerged. (E and F) Fully reprogrammed human embryonic stem cells (hESC)-like colonies were isolated after 21 days. Micrographs (G and I in low magnification) and (H and J in high magnification) show the morphology and quality of the colonies. Scale bars: A-F = 200 μm, G and I = 100 μm, H and J = 400 μm.</p

Differentiation of HiPSCs into neurons and astrocytes.

<p>Representative micrographs showing that differentiated HiPSCs acquired neuronal morphology with extending neuritic processes (A) in phase contrast, and became positive for (B) MAP-2 and (C) βIII-tubulin. (D) Primary human fetal cells differentiate into neurons (green) and GFAP-positive astrocytes (red). Similarly, HiPSCs differentiated not only to neurons but also to GFAP-positive astrocytes. The micrograph in (E) shows a HiPSC-derived mixed culture of neurons (green) and GFAP immune-stained astrocytes (red). (F) A micrograph showing few mature astrocytes positive for S100B. Blue is DAPI for nuclear staining. Scale bar: 200 μm.</p