Additional file 4: of A long non-coding RNA HOTTIP expression is associated with disease progression and predicts outcome in small cell lung cancer patients

Legends of supplementary figures, primers, RNAi sequences, miRNA and antibodies information. (DOC 633 kb

Additional file 1: of A long non-coding RNA HOTTIP expression is associated with disease progression and predicts outcome in small cell lung cancer patients

HOTTIP and miR-574-5p were screened out by microarray and RT-qPCR methods. (JPEG 275 kb

Additional file 2: of A long non-coding RNA HOTTIP expression is associated with disease progression and predicts outcome in small cell lung cancer patients

HOTTIP regulated the cell cycle of SCLC cells. (JPEG 353 kb

The cell viability (IC50) changes after PTRF konckdown.

<p>(A) The cell viabilty of U251AR cells, U251AR cells transfected with shNC and U251AR cells transfected with shPTRF after treatment with 100 µg/mL TMZ for 24 h, 48 h, 72 h, 96 h, and 120 h; (B) The IC50 of U251AR cells and U251AR cells transfected with shNC and shPTRF; (C) The IC50 of U251 cells and U251 cells transfected with shNC and shPTRF. **, P<0.05.</p

Twenty-one differentially expressed proteins in U251 cell line versus U251AR cell line.

<p>Note:</p>a<p>Protein MW, Protein molecular weight;</p>b<p>Fold changes (mean ± SD) of U251 cell line vs. U251AR cell line, which were calculated from the DeCyder/spot volume analysis;</p>c<p>up, up-regulated in the U251AR cell line;</p>d<p>down, down-regulated in the U251AR cell line.</p

The nucleotide sequences of the target miRNA and the unrelated miRNA.

<p>The nucleotide sequences of the target miRNA and the unrelated miRNA.</p

Proteomic analysis of GBM cells by 2D-DIGE.

<p>A representative 2D-DIGE image (merged image) showed the protein profile of U251AR and U251 cells, labeled with Cy3 (green spots) and Cy5 (red spots), respectively. The approximate molecular weight range in the vertical dimension was from 10 to 150 kD. The PI of proteins ranged from 3 to 10. The differently expressed protein spot ID were indicated with white arrows. The protein spot 2421 and 1737 was PTRF and VIM, respectively.</p

Primer sequences used in quantitative RT-PCR analysis.

<p>Primer sequences used in quantitative RT-PCR analysis.</p

Expression levels of PTRF and caveolin1 in GBM patients.

<p>(A) PTRF and Caveolin1 expression in primary GBM and relapsed GBM patients as detected by quantitative RT-PCR (*, P<0.05). (B) The correlation of PTRF and caveolin1 mRNA in the same GBM specimens (2-tailed Pearson correlation, r = 0.766, P<0.01).</p

The biological characteristics of MDR cell line U251AR.

<p>(A) The expression of P-gp was significantly increased in chemoresistant cell line U251AR as detected by Western blot analyses. (B) The mRNA levels of P-gp, MRP1, and BCRP in U251 and U251AR cells were determined by quantitative RT-PCR. Values of three independent experiments were represented as the mean ± SD. *, P<0.05.</p