63 research outputs found

    Effects of GHS on sarcoplasmic reticulum (SR) Ca<sup>2+</sup> content and phospho-phospholamban (p-PLB)/phospholamban (PLB) expression.

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    <p>(A) Illustration of the SR Ca<sup>2+</sup> content measurement protocol (data from cardiomyocytes exposed to ischemia/reperfusion). R represents the emission fluorescence ratio of fura-2 from excitation at 340 and 380nm. Cardiomyocytes were perfused with Tyrode solution containing 1.5 mM CaCl<sub>2</sub> and paced at 0.5 Hz for at least 30 s. 10 mM caffeine was then added to induce SR Ca<sup>2+</sup> release. SR Ca<sup>2+</sup> content, as determined by both (B) Area under curve and (C) amplitude of the caffeine-induced Ca<sup>2+</sup> release, significantly decreased after 20 min ischemia compared with the control group. Ghrelin (G) or hexarelin (H) pre-treatment (pre) and post-treatment (post) significantly increased the SR Ca<sup>2+</sup> content after 20 min ischemia (B and C), but introduction of ghrelin into the perfusion system at 40 min and lasting for 10 min (G post control) had no effect on the SR Ca<sup>2+</sup> content of the cells isolated from the normal perfused heart. (D) The time-to-90% decay of caffeine-induced increases in [Ca<sup>2+</sup>]<sub>i</sub> mainly reflect the Ca<sup>2+</sup> clearance ability of the Na<sup>+</sup>/Ca<sup>2+</sup> exchanger (NCX). n  =  18, 44, 44, 33, 38, 41 and 38 cells/3 mice in G post control, control, ischemia, G pre, G post, H pre and H post, respectively. (E) Representative western blots of the total phospholamban (PLB) and the phosphorylated PLB (p-PLB) in 6 groups and (F) the densitometric quantification of ratio of p-PLB/PLB (expressed as fold increase relative to control). n  =  5 mice in each group. Data are shown as means ± S.E.M. and analyzed by one-way ANOVA with <i>Tukey’s post hoc</i> test. *<i>P</i> &lt; 0.05, **<i>P</i> &lt; 0.01, *** <i>P</i> &lt; 0.001 vs ischemic group.</p

    Typical images from the proposed dataset along with ground truth rectangles.

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    <p>Notice the red rectangles. They indicate the texts within them are labeled as difficult (due to blur or occlusion).</p

    Role of GHS-R1a in the cardiac effects of hexarelin (H) and ghrelin (G).

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    <p>R represents the emission fluorescence ratio of fura-2 from excitation at 340 and 380nm. The GHS-R1a antagonists, [D-Lys3]-GHRP-6 (GHRP6, 200 nM) and BIM28163 (BIM,100 nM), completely blocked the effects of 1 nM hexarelin (H) and 10 nM ghrelin (G) post-treatment (post) on sarcomere shortening (A) and [Ca<sup>2+</sup>]<sub>i</sub> transients (B). These peptides alone did not produce any noticeable change in sarcomere shortening (A) and [Ca<sup>2+</sup>]<sub>i</sub> transients (B). For sarcomere shortening experiments, n  =  99, 84, 52, 95, 46,100, 50 and 61 in control, ischemic, GHRP6, G post, G post +GHRP6, BIM, H post and H post+BIM groups, respectively. For [Ca<sup>2+</sup>]<sub>i</sub> transient experiments, n  =  72, 80, 55, 103, 69, 60, 66 and 68 in control, ischemic, GHRP6, G post, G post +GHRP6, BIM, H post and H post+BIM groups, respectively. Data were analyzed by one-way ANOVA with <i>Tukey's post hoc</i> test, and expressed as means ± S.E.M. *<i>P</i> &lt; 0.05, ** <i>P</i> &lt; 0.01, *** <i>P</i> &lt; 0.001.</p

    Detected single characters in images.

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    <p>Images are from the ICDAR dataset <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070173#pone.0070173-Lucas1" target="_blank">[47]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070173#pone.0070173-Lucas2" target="_blank">[48]</a>.</p

    Performances of different text detection methods evaluated on texts of different languages.

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    <p>Performances of different text detection methods evaluated on texts of different languages.</p
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