Phenotypic and Genotypic Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Related to Persistent Endovascular Infection.

Persistent methicillin-resistant Staphylococcus aureus (MRSA) bacteremia (PB) represents an important subset of S. aureus infection and correlates with poor clinical outcomes. MRSA isolates from patients with PB differ significantly from those of resolving bacteremia (RB) with regard to several in vitro phenotypic and genotypic profiles. For instance, PB strains exhibit less susceptibility to cationic host defense peptides and vancomycin (VAN) killing under in vivo-like conditions, greater damage to endothelial cells, thicker biofilm formation, altered growth rates, early activation of many global virulence regulons (e.g., sigB, sarA, sae and agr) and higher expression of purine biosynthesis genes (e.g., purF) than RB strains. Importantly, PB strains are significantly more resistant to VAN treatment in experimental infective endocarditis as compared to RB strains, despite similar VAN minimum inhibitory concentrations (MICs) in vitro. Here, we review relevant phenotypic and genotypic characteristics related to the PB outcome. These and future insights may improve our understanding of the specific mechanism(s) contributing to the PB outcome, and aid in the development of novel therapeutic and preventative measures against this life-threatening infection

Platelets in defense against bacterial pathogens

Platelets interact with bacterial pathogens through a wide array of cellular and molecular mechanisms. The consequences of this interaction may significantly influence the balance between infection and immunity. On the one hand, recent data indicate that certain bacteria may be capable of exploiting these interactions to gain a virulence advantage. Indeed, certain bacterial pathogens appear to have evolved specific ways in which to subvert activated platelets. Hence, it is conceivable that some bacterial pathogens exploit platelet responses. On the other hand, platelets are now known to possess unambiguous structures and functions of host defense effector cells. Recent discoveries emphasize critical features enabling such functions, including expression of toll-like receptors that detect hallmark signals of bacterial infection, an array of microbicidal peptides, as well as other host defense molecules and functions. These concepts are consistent with increased risk and severity of bacterial infection as correlates of clinical abnormalities in platelet quantity and quality. In these respects, the molecular and cellular roles of platelets in host defense against bacterial pathogens are explored with attention on advances in platelet immunobiology

Evaluation of Structural and Thermal Properties of Rubber and HDPE for Utilization as Binder Modifier

Today, high-performance requirements for asphalt pavements demand enhanced properties for bitumen to withstand the environmental condition and traffic demand. On the other hand, the rapid growth in population and economy results in a continuously increased material consumption, and subsequently waste generation. Among various waste materials, rubber and plastic, including high-density polyethylene (HDPE), constitute some part of the non-biodegradable solid wastes worldwide. Because of the great difficulties in managing the non-biodegradable wastes and the required volume of bitumen, the idea of using plastic and rubber as bitumen modifier in new asphalt mixtures appears to be an effective and meaningful utilization of these materials. As binder plays an important role in the final performance of the asphalt mixture, an understanding of modified binder properties is essential in designing an asphalt mixture. To this point, since compatibility of asphalt mixture with polymer is the most important factor in the blend of polymers and asphalt, the properties of the waste polymers were evaluated in this ongoing research by means of advanced thermal analysis and scanning electron microscope (SEM). This chapter presents the results of this experimental study to evaluate the properties of polymers as potential modifier for virgin bitumen in asphalt mixture

Estimates of efficiency of Dorper and Rambouillet lambs.

Feed intake and body weight were recorded on thirty-seven Dorper ram lambs and forty-two Rambouillet ram lambs from weaning to 61kg of body weight to determine feed conversion efficiency. The data were collected over a two year period. Lambs were progeny of 6 unrelated sires per breed. Lambs were approximately 90 days of age and 31kg body weight at the start of the trial. Lambs were fed a commercially prepared, pelleted diet with an average crude protein content of 16% and TDN of 70.5%. Lambs were divided into groups of 10-11 containing both breeds, and fed using FIRE (Feed Intake Recording Equipment, Osborne Industries, Inc, Osborne, KS, USA). Lambs were identified using a unique electronic identification ear tag transponder. Feed intake was recorded electronically and lambs were weighed every two weeks. Data were analyzed using SAS PROC MIXED with a model that included breed and birth type as fixed effects, a random effect for sire, and starting weight as a covariate. In both years of the trial, breed was not found to have a statistically significant effect on ADG or FCE (p>0.05). Post weaning feed efficiency was similar between Dorper and Rambouillet lambs. The Dorpers’ total ADG was 340 g/d; the Rambouillets’ was 342 g/d. The Dorpers’ average FCE was 0.159; the Rambouillets’ was 0.158. Over the post weaning period studied there was no advantage shown by either breed

Comparing genome scans among species of the stickleback order reveals three different patterns of genetic diversity

Comparing genome scans among species is a powerful approach for investigating the patterns left by evolutionary processes. In particular, this offers a way to detect candidate genes that drive convergent evolution. We compared genome scan results to investigate if patterns of genetic diversity and divergence are shared among divergent species within the stickleback order (Gasterosteiformes): the threespine stickleback (Gasterosteus aculeatus), ninespine stickleback (Pungitius pungitus), and tubesnout (Aulorhynchus flavidus). Populations were sampled from the southern and northern edges of each species’ range, to identify patterns associated with latitudinal changes in genetic diversity. Weak correlations in genetic diversity (FST and expected heterozygosity) and three different patterns in the genomic landscape were found among these species. Additionally, no candidate genes for convergent evolution were detected. This is a counterexample to the growing number of studies that have shown overlapping genetic patterns, demonstrating that genome scan comparisons can be noisy due to the effects of several interacting evolutionary forces