Biodegradable Polyurethane Pressure-Sensitive Adhesives Using Biobased Polyols with Distinct Intrinsic Properties

Polyurethane pressure-sensitive adhesives (PSAs) were synthesized by reacting isophorone diisocyanate (IPDI) with mixtures of different biobased polyols, where polylactide diol (PLA 1000) acted as a hard polyol and cashew nut shell liquid (CNSL)-derived diol and castor oil (CO) acted as soft segments. Besides, CO played another role in the synthesis of the polyurethanes as a cross-linker to enhance the cohesion of the prepared PSAs. The effects of different ratios of polyols and NCO/OH on microphase separation, thermal, rheological, and adhesive properties of the polyurethane were comprehensively studied. A balanced PSA with excellent tack and sufficient peel strength was prepared by adjusting the ratio of different polyols at PLA1000/CNSL diol/CO = 4:4:2 and a suitable NCO/OH value of 0.70. Eventually, the biodegradability of PSA 442-0.70 was investigated, and the biodegradation rate reached 64.47% within 8 weeks, making it a promising environmentally friendly adhesive tape for packaging applications

Additional file 1 of An ovalbumin fusion strategy to increase recombinant protein secretion in chicken eggs

Additional file 1: Supplemental Fig. 1. Ovalbumin-EGFP fusion protein structures predicted by alphafold2 software. A. Structure of ovalbumin-EGFP fusion proteins with GS linker. B. Structure of ovalbumin-EGFP fusion proteins with (GS)3 linker. A. Structure of ovalbumin-EGFP fusion proteins with 32aa linker. A. Structure of ovalbumin-EGFP fusion proteins with (EAAAK)5 linker. Supplemental Fig. 2. Plasmids used in OVAL gene modified or CAG-EGFP chicken. A. Structure of CRISPR/Cas9 and donor plasmid used in ovalbumin locus site-specific gene integration. B. PiggayBac plasmid used in CAG-EGFP transgene chicken. Supplemental Fig. 3. EGFP fluorescence detection of PGCs derived from CAG-EGFP chicken. Supplemental Fig. 4. mCherry fluorescence detection of eggs from OVAL-E3-EGFP chicken. Supplemental Fig. 5. Immune fluorescence (IF) analysis of oviduct tissues from WT three-yellow chicken, CAG-EGFP chicken, and OVAL-E3-EGFP chicken. A. Ovalbumin antibody IF analysis of oviducts from WT three-yellow chicken, CAG-EGFP chicken, and OVAL-E3-EGFP chicken. B. EGFP antibody IF analysis of chicken oviduct from OVAL-E3-EGFP chicken. Supplemental Fig. 6. EGFP immune fluorescence analysis of oviduct tissues from wild type three-yellow chicken and CAG-EGFP chicken. Supplemental Fig. 7. FACS of EGFP+/EGFP- cells from OVAL-E3-EGFP chicken oviducts

Additional file 1 of Undetectable off-target effects induced by FokI catalytic domain in mouse embryos

Additional file 1. Supplementary figures S1-S11 and tables S1-S4