RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming - Fig 5

<p>Scatter plot of DEGs in (a) A vs. C; (b) B vs. D; (c) A vs. B; (d) C vs. D and GO functional classification of DEGs in (e) A vs. C and (f) B vs. D. Note: For A vs. C, A is the control group. Red points indicate genes up-regulated in C relative to A, green points represent genes down-regulated in C relative to A, and blue points represent genes that showed no differences or fold change below 2.</p

Expression of selected genes in real-time quantitative RT-PCR.

<p>Expression of selected genes in real-time quantitative RT-PCR.</p

Summary of mapping results of four samples (mapping to reference genome).

<p>Summary of mapping results of four samples (mapping to reference genome).</p

Cluster image of DEG levels of transcription factor activity genes expressed in the nucleus.

<p>Each column represents one sample, and each row represents one gene. Red indicates up-regulation and green indicates down-regulation.</p

The shape of blastocysts generated from parthenogenesis, enucleated oocyte SCNT and intact oocyte SCNT.

<p>The shape of blastocysts generated from parthenogenesis, enucleated oocyte SCNT and intact oocyte SCNT.</p

The influence come from mechanical trauma to the cleavage rate and blastocyst rate and blastocyst cell number of parthenogenesis.

<p>The influence come from mechanical trauma to the cleavage rate and blastocyst rate and blastocyst cell number of parthenogenesis.</p

Developmental potential of embryos reconstructed by injection of cumulus cells into intact and enucleated oocytes.

<p>Developmental potential of embryos reconstructed by injection of cumulus cells into intact and enucleated oocytes.</p

The cleavage rate and blastocyst rate of intact oocyte NT after genome exchage.

<p>The cleavage rate and blastocyst rate of intact oocyte NT after genome exchage.</p

Karyotyping of cumulus cells, enucleated oocyte SCNT embryos and intact oocyte SCNT embryos.

<p>Karyotyping of cumulus cells, enucleated oocyte SCNT embryos and intact oocyte SCNT embryos.</p

The cell number of blastocyst generated from parthenogenesis,enucleated oocyte NT and intact oocyte NT.

<p>The cell number of blastocyst generated from parthenogenesis,enucleated oocyte NT and intact oocyte NT.</p