Phase-Tunable Synthesis of Monodisperse YPO₄:Ln³⁺ (Ln = Ce, Eu, Tb) Micro/Nanocrystals via Topotactic Transformation Route with Multicolor Luminescence Properties

A novel aqueous-based and phase-selected synthetic strategy toward YPO₄:Ln³⁺ (Ln = Ce, Eu, Tb) micro/nanocrystals was developed by selecting specific precursors whose structure topotactically matches with the target ones. It was found that layered yttrium hydroxide (LYH) induced the formation of hexagonal-phased <i>h</i>-YPO₄·0.8H₂O with the crystalline relationship of [001]­LYH<b>//</b>[0001]<i>h</i>-YPO₄·0.8H₂O, while the amorphous Y­(OH)­CO₃ favored the formation of tetragonal-phased <i>t</i>-YPO₄. We also systematically investigated the influence of Na₂CO₃/NaH₂PO₄ feeding ratio on the evolutions of morphology and size of the <i>h</i>-YPO₄·0.8H₂O sample, and we also obtained a novel mesoporous nanostructure for <i>t</i>-YPO₄ single crystalline with closed octahedron shape for the first time. Besides, the multicolor and phase-dependent luminescence properties of the as-obtained <i>h</i>-YPO₄·0.8H₂O and <i>t</i>-YPO₄ micro/nanocrystals were also investigated in detail. Our work may provide some new guidance in synthesis of nanocrystals with target phase structure by rational selection of precursor with topotactic structural matching

DataSheet_4_Comprehensive analysis of lactate-related gene profiles and immune characteristics in lupus nephritis.csv

ObjectivesThe most frequent cause of kidney damage in systemic lupus erythematosus (SLE) is lupus nephritis (LN), which is also a significant risk factor for morbidity and mortality. Lactate metabolism and protein lactylation might be related to the development of LN. However, there is still a lack of relative research to prove the hypothesis. Hence, this study was conducted to screen the lactate-related biomarkers for LN and analyze the underlying mechanism.MethodsTo identify differentially expressed genes (DEGs) in the training set (GSE32591, GSE127797), we conducted a differential expression analysis (LN samples versus normal samples). Then, module genes were mined using WGCNA concerning LN. The overlapping of DEGs, critical module genes, and lactate-related genes (LRGs) was used to create the lactate-related differentially expressed genes (LR-DEGs). By using a machine-learning algorithm, ROC, and expression levels, biomarkers were discovered. We also carried out an immune infiltration study based on biomarkers and GSEA.ResultsA sum of 1259 DEGs was obtained between LN and normal groups. Then, 3800 module genes in reference to LN were procured. 19 LR-DEGs were screened out by the intersection of DEGs, key module genes, and LRGs. Moreover, 8 pivotal genes were acquired via two machine-learning algorithms. Subsequently, 3 biomarkers related to lactate metabolism were obtained, including COQ2, COQ4, and NDUFV1. And these three biomarkers were enriched in pathways ‘antigen processing and presentation’ and ‘NOD-like receptor signaling pathway’. We found that Macrophages M0 and T cells regulatory (Tregs) were associated with these three biomarkers as well.ConclusionOverall, the results indicated that lactate-related biomarkers COQ2, COQ4, and NDUFV1 were associated with LN, which laid a theoretical foundation for the diagnosis and treatment of LN.</p

DataSheet_1_Comprehensive analysis of lactate-related gene profiles and immune characteristics in lupus nephritis.csv

ObjectivesThe most frequent cause of kidney damage in systemic lupus erythematosus (SLE) is lupus nephritis (LN), which is also a significant risk factor for morbidity and mortality. Lactate metabolism and protein lactylation might be related to the development of LN. However, there is still a lack of relative research to prove the hypothesis. Hence, this study was conducted to screen the lactate-related biomarkers for LN and analyze the underlying mechanism.MethodsTo identify differentially expressed genes (DEGs) in the training set (GSE32591, GSE127797), we conducted a differential expression analysis (LN samples versus normal samples). Then, module genes were mined using WGCNA concerning LN. The overlapping of DEGs, critical module genes, and lactate-related genes (LRGs) was used to create the lactate-related differentially expressed genes (LR-DEGs). By using a machine-learning algorithm, ROC, and expression levels, biomarkers were discovered. We also carried out an immune infiltration study based on biomarkers and GSEA.ResultsA sum of 1259 DEGs was obtained between LN and normal groups. Then, 3800 module genes in reference to LN were procured. 19 LR-DEGs were screened out by the intersection of DEGs, key module genes, and LRGs. Moreover, 8 pivotal genes were acquired via two machine-learning algorithms. Subsequently, 3 biomarkers related to lactate metabolism were obtained, including COQ2, COQ4, and NDUFV1. And these three biomarkers were enriched in pathways ‘antigen processing and presentation’ and ‘NOD-like receptor signaling pathway’. We found that Macrophages M0 and T cells regulatory (Tregs) were associated with these three biomarkers as well.ConclusionOverall, the results indicated that lactate-related biomarkers COQ2, COQ4, and NDUFV1 were associated with LN, which laid a theoretical foundation for the diagnosis and treatment of LN.</p

Mutational spectrum of the phenylalanine hydroxylase gene in patients with phenylketonuria in the central region of China

<p>Phenylketonuria (PKU, OMIM 261600) caused by phenylalanine hydroxylase (PAH) deficiency is an autosomal recessive disease that is characterized by abnormalities of phenylalanine metabolism. In this study, a total of 77 patients, originating from the central region of China and who were diagnosed with PAH deficiency at the third affiliated hospital of Zhengzhou University, were enrolled in this study. The 13 exons and 12 flanking introns of the <i>PAH</i> gene were analyzed by Sanger sequencing and next generation sequencing. The sequencing data were aligned to the hg19, PAHvdb and HGMD databases to characterize the genotypes of PKU patients, and genotype–phenotype correlations and BH4 responsiveness predictions were performed using BIOPKUdb. In total, 149 alleles were characterized among the 154 PKU alleles. These mutations were located in exons 2–13, and intron 12 of the <i>PAH</i> gene, with a relative frequency of ≥5%, for EX6-96A>G, p.R241C, p.R243Q, p.V399V and p.R53H. Additionally, a novel variant, p.D84G, was identified. The genotype correlated with clinical symptoms in 33.3–100% of the cases, depending on the disease severity, and BH4 responsiveness predictions show that only five patients with MHP-PKU and one patient with Mild-PKU were predicted to be BH4 responsive. In conclusion, we have characterized the mutational spectrum of <i>PAH</i> in the central region of China and have identified a novel mutation. The hotspot mutation information might be useful for screening, diagnosis and treatment of PKU.</p

Additional file 1 of Starvation induces hepatopancreas atrophy in Chinese mitten crab (Eriocheir sinensis) by inhibiting angiogenesis

Supplementary Material

Supplementary document for Learning based Compressive Snapshot Spectral Light Field Imaging with RGB Sensors - 6605677.pdf

Supplemental Documen

Emission Enhancement and Color Tuning for GdVO₄:Ln³⁺ (Ln = Dy, Eu) by Surface Modification at Single Wavelength Excitation

The surface modification can realize systematically the emission enhancement of GdVO₄:Ln³⁺ (Ln = Dy, Eu) microstructures and multicolor emission at single component. The structure, morphology, composition, and the surface ligands modification of as-prepared samples were studied in detail. It is found that the surface-modified ligands can act as sensitizer to improve the emission of the Eu³⁺ and Dy³⁺ ions via the energy transfer besides the VO₄^3––Eu³⁺/Dy³⁺ process. More importantly, under a single wavelength excitation, the emission color can be effectively tuned by manipulating the doping ratio of the Eu³⁺ ions in the internal crystal lattice and the Tb³⁺ ions in the external surface ligands, simultaneously. And further, multicolor emissions are obtained under single wavelength excitation due to the high overlapping between the VO₄^3– absorption and the π–π* electron transition of the ligands. These findings may open new avenues to design and develop new highly efficient luminescent materials

High-Throughput Screening for GPR119 Modulators Identifies a Novel Compound with Anti-Diabetic Efficacy in <i>db/db</i> Mice

<div><p>G protein-coupled receptor 119 (GPR119) is highly expressed in pancreatic β cells and enteroendocrine cells. It is involved in glucose-stimulated insulin secretion and glucagon-like peptide-1 (GLP-1) release, thereby representing a promising target for the treatment of type 2 diabetes. Although a number of GPR119 agonists were developed, no positive allosteric modulator (PAM) to this receptor has been reported. Here we describe a high-throughput assay for screening GPR119 PAMs and agonists simultaneously. Following screening of a small molecule compound library containing 312,000 synthetic and natural product-derived samples, one potent GPR119 agonist with novel chemical structure, MW1219, was identified. Exposure of MIN6 and GLUTag cells to MW1219 enhanced glucose-stimulated insulin secretion and GLP-1 release; once-daily oral dosing of MW1219 for 6 weeks in diabetic <i>db/db</i> mice reduced hemoglobin A1c (HbA1c) and improved plasma glucose, insulin and GLP-1 levels; it also increased glucose tolerance. The results demonstrate that MW1219 is capable of effectively controlling blood glucose level and may have the potential to be developed as a new class of anti-diabetic agents.</p></div