Transition-Metal-Free Sonogashira-Type Cross-Coupling of Alkynes with Fluoroarenes

A novel, inexpensive, and efficient palladium-, copper-, ligand-, and amine-free Sonogashira-type cross-coupling reaction of terminal alkynes with unreactive aryl fluorides in the presence of sodium, sodium methoxide, and calcium hydroxide under the assistance of a Grignard reagent was developed. A plausible mechanism was also suggested

Copper-Catalyzed Coupling Cyclization of <i>gem</i>-Difluoroalkenes with Activated Methylene Carbonyl Compounds: Facile Domino Access to Polysubstituted Furans

A novel and efficient CuI-catalyzed synthesis of 2,3,5-trisubstituted furans was developed via coupling cyclization of <i>gem</i>-difluoroalkenes with active methylene carbonyl compounds such as 1,3-dicarbonyl compounds, acetoacetonitrile, and phenylsulfonylacetone with the assistance of a base. Commercial availability of substrates or reagents, good to high isolated yields, and excellent functional group compatibility make this transformation a powerful tool for the synthesis of various furans. A plausible mechanism involving the allenyl ketone is suggested

Copper-Catalyzed Coupling Cyclization of <i>gem</i>-Difluoroalkenes with Activated Methylene Carbonyl Compounds: Facile Domino Access to Polysubstituted Furans

A novel and efficient CuI-catalyzed synthesis of 2,3,5-trisubstituted furans was developed via coupling cyclization of <i>gem</i>-difluoroalkenes with active methylene carbonyl compounds such as 1,3-dicarbonyl compounds, acetoacetonitrile, and phenylsulfonylacetone with the assistance of a base. Commercial availability of substrates or reagents, good to high isolated yields, and excellent functional group compatibility make this transformation a powerful tool for the synthesis of various furans. A plausible mechanism involving the allenyl ketone is suggested

Synthesis of <i>N</i>‑(α-Fluorovinyl)azoles by the Reaction of Difluoroalkenes with Azoles

A mild and versatile method for the construction of C–N bonds by the reaction of (2,2-difluorovinyl)­arenes with various N–H-containing heterocycles in the presence of K₃PO₄ has been developed. The reaction proceeded efficiently at room temperature (25 °C) affording the (<i>E</i>)-<i>N</i>-α-fluorovinyl derivatives of azoles <b>3</b> in good to excellent yields with relatively high stereoselectivity

Additional file 1 of RUNX1 promotes angiogenesis in colorectal cancer by regulating the crosstalk between tumor cells and tumor associated macrophages

Additional file 1: Supplementary Fig. 1. RUNX1 expression is upregulated in CRC. (A) Expression profiles of RUNX1 in diverse cancers and normal tissues using the Oncomine database (http://www.oncomine.org/resource/login.html). (B) Expression profiles of RUNX1 in diverse cancers and normal tissues using the online website TIMER (http://cistrome.shinyapps.io/timer/). (C) RUNX1 is upregulated in colorectal cancer using the GEPIA analysis (http://gepia.cancer-pku.cn/). (D) IHC data from the Human Protein Atlas Analysis (HPA) observed RUNX1 expression in normal colon and CRC tissues (http://www.Proteinatlas.org/). (E) Correlation between RUNX1 expression and macrophage infiltrations in COAD. (F) Correlation between the mRNA expression of RUNX1 and CD68, CD206, IL-10 in CRC tissues (n = 30). (G) The RUNX1 expression in 293T, FHC, SW480, SW620, HT29, HCT116, RKO and LoVo cells were detected by western blotting. (H) The full picture of Figure 1H. (I-L) Stably transfected CRC cells were generated. Western blotting and RT-qPCR analysis of the expression of RUNX1 in HCT116, RKO and SW480 cells