6 research outputs found
Chemical Imaging of Latent Fingerprints by Mass Spectrometry Based on Laser Activated Electron Tunneling
Identification of endogenous and
exogenous chemicals
contained in latent fingerprints is important for forensic science
in order to acquire evidence of criminal identities and contacts with
specific chemicals. Mass spectrometry has emerged as a powerful technique
for such applications without any derivatization or fluorescent tags.
Among these techniques, MALDI (Matrix Assisted Laser Desorption Ionization)
provides small beam size but has interferences with MALDI matrix materials,
which cause ion suppressions as well as limited spatial resolution
resulting from uneven distribution of MALDI matrix crystals with different
sizes. LAET (Laser Activated Electron Tunneling) described in this
work offers capabilities for chemical imaging through electron-directed
soft ionization. A special film of semiconductors has been designed
for collection of fingerprints. Nanoparticles of bismuth cobalt zinc
oxide were compressed on a conductive metal substrate (Al or Cu sticky
tape) under 10 MPa pressure. Resultant uniform thin films provide
tight and shining surfaces on which fingers are impressed. Irradiation
of ultraviolet laser pulses (355 nm) on the thin film instantly generates
photoelectrons that can be captured by adsorbed organic molecules
and subsequently cause electron-directed ionization and fragmentation.
Imaging of latent fingerprints is achieved by visualization of the
spatial distribution of these molecular ions and structural information-rich
fragment ions. Atomic electron emission together with finely tuned
laser beam size improve spatial resolution. With the LAET technique,
imaging analysis not only can identify physical shapes but also reveal
endogenous metabolites present in females and males, detect contacts
with prohibited substances, and resolve overlapped latent fingerprints
Laser Activated Electron Tunneling Based Mass Spectrometric Imaging of Molecular Architectures of Mouse Brain Revealing Regional Specific Lipids
A comprehensive
description of overall brain architecture at the
molecular level is essential for understanding behavioral and cognitive
processes in health and diseases. Although fluorescent labeling of
target proteins has been successfully established to visualize a brain
connectome, the molecular basis for diverse neurophysiological phenomena
remains largely unknown. Here we report a brain-wide, molecular-level,
and microscale imaging of endogenous metabolites, in particular, lipids
of mouse brain by using laser activated electron tunneling (LAET)
and mass spectrometry. In this approach, atomic electron emission
along with finely tuned laser beam size provides high resolution that
can be down to the sub-micrometer level to display spatial distribution
of lipids in mouse brain slices. Electron-directed soft ionization
has been achieved through exothermal capture of tunneling photoelectrons
as well as unpaired electron-initiated chemical bond cleavages. Regionally
specific lipids including saturated, mono-unsaturated, and poly-unsaturated
fatty acids as well as other lipids, which may be implicated in neurological
signaling pathways, have been discovered by using this laser activated
electron tunneling based mass spectrometric imaging (LAET-MSI) technique
DataSheet_1_Diagnostic value of cerebrospinal fluid human epididymis protein 4 for leptomeningeal metastasis in lung adenocarcinoma.doc
BackgroundThe diagnosis of lung adenocarcinoma (LUAD) leptomeningeal metastasis (LM) remains a clinical challenge. Human epididymis protein 4 (HE4) functions as a novel tumor biomarker for cancers. This study aimed to assess the diagnostic value of cerebrospinal fluid (CSF) HE4, and combined with CEACAM6, for LUAD LM.MethodsThe CSF HE4 protein level was measured in two independent cohorts by electrochemiluminescence. Test cohort included 58 LUAD LM patients, 22 LUAD patients without LM (Wiot-LM), and 68 normal controls. Validation cohort enrolled 50 LUAD LM patients and 40 normal controls, in parallel with Wiot-LM patients without brain metastases (19 Wiot-LM/BrM patients) or with BrM (26 BrM patients). The CSF level of CEA, CA125, CA153, CA199, CA724, NSE and ProGRP of these samples was measured by electrochemiluminescence, whereas the CSF CEACAM6 level was detected by enzyme-linked immunosorbent assay (ELISA). In addition, the serum level of these biomarkers was detected by same method as CSF.ResultsThe level of HE4 or CEACAM6 in CSF samples from LUAD LM patients was significantly higher than those from normal controls and Wiot-LM patients. The HE4 or CEACAM6 level in CSF was higher than that in serum of LM patient. The CSF HE4 or CEACAM6 level for distinguished LM from Wiot-LM showed good performance by receiver-operating characteristic analysis. The better discriminative power for LM was achieved when HE4 was combined with CEACAM6. In addition, the CSF HE4 and CEACAM6 level showed little or no difference between Wiot-LM/BrM and BrM patients, the BrM would not significantly influence the HE4 or CEACAM6 level in CSF. The diagnostic power of CSF CA125, CA153, CA199, CA724, NSE and ProGRP for LUAD LM were not ideal.ConclusionThe combination with HE4 and CEACAM6 has the promising application for the diagnosis of LUAD LM.</p
Table_1_Diagnostic value of cerebrospinal fluid human epididymis protein 4 for leptomeningeal metastasis in lung adenocarcinoma.xlsx
BackgroundThe diagnosis of lung adenocarcinoma (LUAD) leptomeningeal metastasis (LM) remains a clinical challenge. Human epididymis protein 4 (HE4) functions as a novel tumor biomarker for cancers. This study aimed to assess the diagnostic value of cerebrospinal fluid (CSF) HE4, and combined with CEACAM6, for LUAD LM.MethodsThe CSF HE4 protein level was measured in two independent cohorts by electrochemiluminescence. Test cohort included 58 LUAD LM patients, 22 LUAD patients without LM (Wiot-LM), and 68 normal controls. Validation cohort enrolled 50 LUAD LM patients and 40 normal controls, in parallel with Wiot-LM patients without brain metastases (19 Wiot-LM/BrM patients) or with BrM (26 BrM patients). The CSF level of CEA, CA125, CA153, CA199, CA724, NSE and ProGRP of these samples was measured by electrochemiluminescence, whereas the CSF CEACAM6 level was detected by enzyme-linked immunosorbent assay (ELISA). In addition, the serum level of these biomarkers was detected by same method as CSF.ResultsThe level of HE4 or CEACAM6 in CSF samples from LUAD LM patients was significantly higher than those from normal controls and Wiot-LM patients. The HE4 or CEACAM6 level in CSF was higher than that in serum of LM patient. The CSF HE4 or CEACAM6 level for distinguished LM from Wiot-LM showed good performance by receiver-operating characteristic analysis. The better discriminative power for LM was achieved when HE4 was combined with CEACAM6. In addition, the CSF HE4 and CEACAM6 level showed little or no difference between Wiot-LM/BrM and BrM patients, the BrM would not significantly influence the HE4 or CEACAM6 level in CSF. The diagnostic power of CSF CA125, CA153, CA199, CA724, NSE and ProGRP for LUAD LM were not ideal.ConclusionThe combination with HE4 and CEACAM6 has the promising application for the diagnosis of LUAD LM.</p
Additional file 1: of Clinical study of children with Takayasu arteritis: a retrospective study from a single center in China
Brief comparison of PVAS and ITAS-2010. (DOCX 16 kb
DataSheet_1_Etiologic characteristics revealed by mNGS-mediated ultra-early and early microbiological identification in airway secretions from lung transplant recipients.xlsx
BackgroundPost-operative etiological studies are critical for infection prevention in lung transplant recipients within the first year. In this study, mNGS combined with microbial culture was applied to reveal the etiological characteristics within one week (ultra-early) and one month (early) in lung transplant recipients, and the epidemiology of infection occurred within one month.MethodsIn 38 lung transplant recipients, deep airway secretions were collected through bronchofiberscope within two hours after the operation and were subjected to microbial identification by mNGS and microbial culture. The etiologic characteristics of lung transplant recipients were explored. Within one month, the infection status of recipients was monitored. The microbial species detected by mNGS were compared with the etiological agents causing infection within one month.ResultsThe detection rate of mNGS in the 38 airway secretions specimens was significantly higher than that of the microbial culture (PConclusionBased on the mNGS-reported pathogens in airway secretions samples collected within two hours, the initial empirical anti-infection regimes covering the bacteria and fungi are reasonable. The existence of bacteria with MDR forecasts the high risk of infection within 48 hours after transplant, reminding us of the necessity to adjust the antimicrobial strategy. The predictive role of mNGS performed within two hours in etiological agents is time-limited, suggesting continuous pathogenic identification is needed after lung transplant.</p