27 research outputs found
Benigne perivaginale Raumforderungen: Ătiologie, Diagnostik und therapeutisches Management
Einleitung:
Benigne perivaginale Raumforderungen (PVRF) sind relativ selten. Treten sie auf, stellen sie in vielen FĂ€llen eine diagnostische und therapeutische Herausforderung dar. VielfĂ€ltige, sich oftmals ĂŒberlappende Symptome, sowie ein mangelndes Bewusstsein fĂŒr diese seltenen EntitĂ€ten tragen maĂgeblich dazu bei. Eine inkorrekte oder verspĂ€tete Diagnose kann mit Inkontinenz, Schmerzen, Rezidiven und weiteren Komplikationen einhergehen und den Leidensweg fĂŒr die betroffenen Patientinnen unnötig verlĂ€ngern. In seltenen FĂ€llen kann es zu einer malignen Transformation kommen. Ziel dieser Studie ist es, ein Bewusstsein fĂŒr diese EntitĂ€ten zu schaffen sowie eine akkurate Diagnostik und Versorgung aufzuzeigen.
Material und Methoden:
Aus den OP-BĂŒchern der UniversitĂ€tsfrauenklinik TĂŒbingen wurden ĂŒber einen Zeitraum von fĂŒnf Jahren die Art und die Anzahl der durchgefĂŒhrten urogynĂ€kologischen Eingriffe im Allgemeinen, sowie die aufgrund einer benignen PVRF erfolgten Eingriffe im Speziellen erhoben. Aus den Krankenunterlagen wurden Diagnostik, Therapie, Histologie und postoperatives Management zusammengefasst und analysiert. Vaginale Endometriosemanifestationen fanden keine BerĂŒcksichtigung.
Ergebnisse:
Im Zeitraum 2011-2015 wurden an unserer Klinik insgesamt 4157 Frauen einer urogynĂ€kologischen Operation unterzogen, 65 (1,6 %) davon aufgrund benigner PVRF. Die verschiedenen EntitĂ€ten variierten erheblich in ihrer GröĂe, Konfiguration und KomplexitĂ€t. Die gröĂte PVRF betrug 10 cm. PVRF traten einzeln oder multipel auf. Sie waren asymptomatisch (21,2 %) oder gingen mit einem breiten Spektrum an Symptomen einher (78,8 %). Anamnese, klinische Untersuchung, Becken-boden-Sonographie, Urethrozystoskopie und MRT waren fĂŒr die Diagnostik entscheidend. In allen 65 FĂ€llen wurde die PVRF exzidiert. In einem weiteren Fall bildete sich ein Urethradivertikel vollstĂ€ndig unter konservativer Therapie zurĂŒck.
Fazit:
Anamnese, klinische Untersuchung, Beckenboden-Sonographie, Urethrozystoskopie und MRT sind essentiell fĂŒr die Diagnostik benigner PVRF. Im Falle einer Infektion sollte grundsĂ€tzlich zunĂ€chst eine konservative Therapie erfolgen. Eine komplette Exzision ist bei einem chirurgischen Vorgehen die Therapiemethode der Wahl. Das Bewusstsein fĂŒr und die Vertrautheit mit den verschiedenen EntitĂ€ten ist von herausragender Bedeutung fĂŒr eine korrekte Diagnose und Versorgung. Als SekundĂ€rpathologie muss auf Divertikelsteine sowie auf eine maligne Entartung geachtet werden
Thermoresponsive Mobile Interfaces with Switchable Wettability, Optical Properties, and Penetrability
Liquid-based mobile
interfaces, in which liquids are being utilized as structural long-term
components, have shown their multifunctionality in materials science,
such as the hydration layer of polyelectrolyte brushes used for artificial
implants, stabilized lubricants for antibiofouling, anti-icing, self-cleaning,
optical control, and so forth. However, these currently available
systems do not usually show a response to environmental stimuli. Here,
we describe a strategy for preparing thermoresponsive mobile interfaces
made from novel silicone-based lubricants that display lower critical
solution temperature and demonstrate their capabilities on controlling
in situ water wetting and dewetting, thermo-gating penetration, and
optical properties. These properties allow the mobile films to form
a kind of erasable recording platforms. We foresee diverse applications
in liquid transport, wetting and adhesion control, and transport switching
Thermoresponsive Mobile Interfaces with Switchable Wettability, Optical Properties, and Penetrability
Liquid-based mobile
interfaces, in which liquids are being utilized as structural long-term
components, have shown their multifunctionality in materials science,
such as the hydration layer of polyelectrolyte brushes used for artificial
implants, stabilized lubricants for antibiofouling, anti-icing, self-cleaning,
optical control, and so forth. However, these currently available
systems do not usually show a response to environmental stimuli. Here,
we describe a strategy for preparing thermoresponsive mobile interfaces
made from novel silicone-based lubricants that display lower critical
solution temperature and demonstrate their capabilities on controlling
in situ water wetting and dewetting, thermo-gating penetration, and
optical properties. These properties allow the mobile films to form
a kind of erasable recording platforms. We foresee diverse applications
in liquid transport, wetting and adhesion control, and transport switching
Flexible Polymer Ultra-Fine Fiber with Extreme Toughness
Fiber materials with
multilevel interior structures have myriad applications in many fields
due to their unique properties. In this study, we develop a bioinspired
flexible ultrafine polymer fiber via an integrated microfluidic-electrospinning
technology. The fiber possesses periodic hollow and tubular chambers
with a shell layer of approximately 150 nm in thickness extremely
like natural bamboo. The single fiber with a diameter of âŒ1.5
ÎŒm exhibits the Youngâs modulus ranging from 2 to 7 MPa
measured with atomic force microscopy (AFM). The fiber with periodic
hollow chambers and extreme toughness can find many applications in
medicine, industry, and agriculture
Bioinspired Artificial Single Ion Pump
Bioinspired artificial functional
nanochannels for intelligent
molecular and ionic transport control at the nanoscale have wide potential
applications in nanofluidics, energy conversion, and biosensors. Although
various smart passive ion transport properties of ion channels have
been artificially realized, it is still hugely challenging to achieve
high level intelligent ion transport features in biological ion pumps.
Here we show a unique bioinspired single ion pump based on a cooperative
pH response double-gate nanochannel, whose gates could be opened and
closed alternately/simultaneously under symmetric/asymmetric pH environments.
With the stimulation of the double-gate nanochannel by continuous
switching of the symmetric/asymmetric pH stimuli, the bioinspired
system systematically realized three key ionic transport features
of biological ion pumps, including an alternating gates ion pumping
process under symmetric pH stimuli, transformation of the ion pump
into an ion channel under asymmetric pH stimuli, and a fail-safe ion
pumping feature under both symmetric and asymmetric pH stimuli. The
ion pumping processes could well be reproduced under a concentration
gradient. With the advantages of the extraordinary ionic transport
functions of biological ion pumps, the bioinspired ion pump should
find widespread applicability in active transportation-controlling
smart nanofluidic devices, efficient energy conversions, and seawater
desalinization, and open the way to design and develop novel bioinspired
intelligent artificial nanochannel materials
Additional file 1: Table S1. of Intramuscular injection of human umbilical cord-derived mesenchymal stem cells improves cardiac function in dilated cardiomyopathy rats
Alterations in serum BNP and cTNI levels by doxorubicin treatment. Figure S1. Development of DCM examined by echocardiography. Figure S2. Survival rate of DCM rats during doxorubicin treatment. Figure S3. hUCMSCs examined for growth capacity, differentiation capacity and immune phenotype. Figure S4. Cardiac fibrosis evaluated by Masson staining (DOC 5053 kb
Confirmation of the c.1885_1889delinsGATCATCAACC indel mutation of <i>LDLR</i>.
<p><b>(A)</b> Sequence chromatogram showing the c.1885_1889delinsGATCATCAACC indel mutation of <i>LDLR</i> in the proband. The mutation was numbered according to GenBank NM_000527.4. <b>(B)</b> Protein alignment of mammalian LDLR showing that the regions around the mutation are highly conserved. Numbers on left and right indicate the position of this fragment. The position of the mutation is marked by a black triangle. <b>(C)</b> Summary of the mutation responsible for familial hypercholesterolemia. The positions of the mutation in this study are marked by a red triangle and a black arrow, respectively.</p
Mutant LDLR depresses the uptake of LDL.
<p><b>(A)</b> Hep G2 cells, stably transfected with wild-type or mutant LDLR, were incubated with DiI-LDL and analyzed by fluorescence microscopy. <b>(B)</b> Fluorescence of DiI-LDL was calculated by ImageJ. One hundred cells were counted in each group and the relative fluorescence was averaged. Data was represented as mean value±SD (***p<0.001).</p
Clinical characteristics findings in the family members.
<p>Clinical characteristics findings in the family members.</p
The indel disrupts the glycosylation of LDLR.
<p><b>(A)</b> The wild-type and mutant LDLR in the membrane were labeled with biotin. Then the biotin was precipitated using streptavidin-agarose beads and immunoblotted with anti-FLAG antibodies. ÎČ-actin was used as a loading control. <b>(B)</b> Relative quantification of wild-type and mutant LDLR in the membrane were analyzed using Quantity One 1-D software (Bio-Rad, Hercules, CA, USA). Data was represented as mean value±SD (**p<0.005). <b>(C)</b> Subcellular localization of wild-type and mutant LDLR in Hep G2 cells. Calnexin is used as an endoplasmic reticulum marker.</p