Additional file 2: of Differentially methylated loci in NAFLD cirrhosis are associated with key signaling pathways

Table S2. Top 100 differentially methylated CpG sites (DOCX 30 kb

Additional file 1: of Differentially methylated loci in NAFLD cirrhosis are associated with key signaling pathways

Table S1. CpG islands differentially methylated between normal and fibrotic samples (N = 208) (DOCX 37 kb

Additional file 4: of Differentially methylated loci in NAFLD cirrhosis are associated with key signaling pathways

Table S3. Canonical pathways identified by pathway analysis using all significant CpG islands (N = 208). (DOCX 26 kb

Additional file 3: of Differentially methylated loci in NAFLD cirrhosis are associated with key signaling pathways

Figure S1. Box plot of mean (± standard deviation) methylation β values for CGI associated with gene expression differences in patients with NAFLD fibrosis (n = 11) compared to individuals with normal liver histology (n = 15). (PDF 544 kb

Multistimuli-Regulated Photochemothermal Cancer Therapy Remotely Controlled <i>via</i> Fe₅C₂ Nanoparticles

Stimuli-controlled drug delivery and release is of great significance in cancer therapy, making a stimuli-responsive drug carrier highly demanded. Herein, a multistimuli-controlled drug carrier was developed by coating bovine serum albumin on Fe₅C₂ nanoparticles (NPs). With a high loading of the anticancer drug doxorubicin, the nanoplatform provides a burst drug release when exposed to near-infrared (NIR) light or acidic conditions. <i>In vitro</i> experiment demonstrated a NIR-regulated cell inhibition that is ascribed from cellular uptake of the carrier and the combination of photothermal therapy and enhanced drug release. The carrier is also magnetic-field-responsive, which enables targeted drug delivery under the guidance of a magnetic field and monitors the theranostic effect by magnetic resonance imaging. <i>In vivo</i> synergistic effect demonstrates that the magnetic-driven accumulation of NPs can induce a complete tumor inhibition without appreciable side effects to the treated mice by NIR irradiation, due to the combined photochemotherapy. Our results highlight the great potential of Fe₅C₂ NPs as a remote-controlled platform for photochemothermal cancer therapy

Monodisperse Au–Fe₂C Janus Nanoparticles: An Attractive Multifunctional Material for Triple-Modal Imaging-Guided Tumor Photothermal Therapy

Imaging-guided photothermal therapy (PTT) by combination of imaging and PTT has been emerging as a promising therapeutic method for precision therapy. However, the development of multicomponent nanoplatforms with stable structures for both PTT and multiple-model imaging remains a great challenge. Herein, we synthesized monodisperse Au–Fe₂C Janus nanoparticles (JNPs) of 12 nm, which are multifunctional entities for cancer theranostics. Due to the broad absorption in the near-infrared range, Au–Fe₂C JNPs showed a significant photothermal effect with a 30.2% calculated photothermal transduction efficiency under 808 nm laser irradiation <i>in vitro</i>. Owing to their excellent optical and magnetic properties, Au–Fe₂C JNPs were demonstrated to be advantageous agents for triple-modal magnetic resonance imaging (MRI)/multispectral photoacoustic tomography (MSOT)/computed tomography (CT) both <i>in vitro</i> and <i>in vivo</i>. We found that Au–Fe₂C JNPs conjugated with the affibody (Au–Fe₂C–Z_HER2:342) have more accumulation and deeper penetration in tumor sites than nontargeting JNPs (Au–Fe₂C–PEG) <i>in vivo</i>. Meanwhile, our results verified that Au–Fe₂C–Z_HER2:342 JNPs can selectively target tumor cells with low cytotoxicity and ablate tumor tissues effectively in a mouse model. In summary, monodisperse Au–Fe₂C JNPs, used as a multifunctional nanoplatform, allow the combination of multiple-model imaging techniques and high therapeutic efficacy and have great potential for precision theranostic nanomedicines