Table1_Immunological role and prognostic value of somatostatin receptor family members in colon adenocarcinoma.XLSX

Colon adenocarcinoma (COAD) is among the most prevalent cancers worldwide, ranking as the third most prevalent malignancy in incidence and mortality. The somatostatin receptor (SSTR) family comprises G-protein-coupled receptors (GPCRs), which couple to inhibitory G proteins (Gi and Go) upon binding to somatostatin (SST) analogs. GPCRs are involved in hormone release, neurotransmission, cell growth inhibition, and cancer suppression. However, their roles in COAD remain unclear. This study used bioinformatics to investigate the expression, prognosis, gene alterations, functional enrichment, and immunoregulatory effects of the SSTR family members in COAD. SSTR1-4 are differentially downregulated in COAD, and low SSTR2 expression indicates poor survival. Biological processes and gene expression enrichment of the SSTR family in COAD were further analyzed using the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. A strong correlation was observed between SSTR expression and immune cell infiltration. We also quantified SSTR2 expression in 25 COAD samples and adjacent normal tissues using quantitative real-time polymerase chain reaction. We analyzed its correlation with the dendritic cell–integrin subunit alpha X marker gene. The biomarker exploration of the solid tumors portal was used to confirm the correlation between SSTR2 with immunomodulators and immunotherapy responses. Our results identify SSTR2 as a promising target for COAD immunotherapy. Our findings provide new insights into the biological functions of the SSTR family and their implications for the prognosis of COAD.</p

Rice- or pork-based diets with similar calorie and content result in different rat gut microbiota

<p>Rice is the most important food crop, and pork is the most widely eaten meat in the world. In this study, we compared the gut microbiota of the rats fed with rice or pork mixed diets, which have similar caloric contents. The physiological indices (body weights, hematology, serum chemistry, organ weights and histopathology) of two groups were all within the normal range. Two diets did not induce difference in the diversity of gut bacteria. However, Firmicutes were significantly higher in rice diet group, while Bacteroidetes were enriched in pork diet group. Butyrate and the bacteria enzymes β-glucuronidase, β-glucosidase and nitroreductase in the feces were all drastically higher in pork diet group. This study indicates that different diets with similar calorie and nutritional composition could change the community structure but not the diversity of rat fecal microbiota.</p

Thyroid transcriptomic profiling reveals the differential regulation of lncRNA and mRNA related to prolificacy in small tail han sheep with <i>FecB BB</i> genotype

The thyroid gland is an important endocrine gland in animals that secretes thyroid hormones and acts on various organs throughout the body. lncRNAs are long non-coding RNAs that play an important role in animal reproduction; however, there is a lack of understanding of their expression patterns and potential roles in the thyroid gland of the Small Tail Han (STH) sheep. In this study, we used RNA-Seq technology to examine the transcriptome expression pattern of the thyroid from the luteal phase (LP) and follicular phase (FP) of FecB BB (MM) STH sheep. We identified a total of 122 and 1287 differential expression lncRNAs (DELs) and differential expression mRNAs (DEGs), respectively, which were significantly differentially expressed. These DELs target genes and DEGs can be enriched in several signalling pathways related to the animal reproduction process. The expression profiles of DELs and DEGs in thyroid glands provide a more comprehensive resource for elucidating the reproductive regulatory mechanisms of STH sheep.</p

Liquid Phase Exfoliated MoS₂ Nanosheets Percolated with Carbon Nanotubes for High Volumetric/Areal Capacity Sodium-Ion Batteries

The search for high-capacity, low-cost electrode materials for sodium-ion batteries is a significant challenge in energy research. Among the many potential candidates, layered compounds such as MoS₂ have attracted increasing attention. However, such materials have not yet fulfilled their true potential. Here, we show that networks of liquid phase exfoliated MoS₂ nanosheets, reinforced with 20 wt % single-wall carbon nanotubes (SWNTs), can be formed into sodium-ion battery electrodes with large gravimetric, volumetric, and areal capacity. The MoS₂/SWNT composite films are highly porous, electrically conductive, and mechanically robust due to its percolating carbon nanotube network. When directly employed as the working electrode, they exhibit a specific capacity of >400 mAh/g and volumetric capacity of ∼650 mAh/cm³. Their mechanical stability allows them to be processed into free-standing films with tunable thickness up to ∼100 μm, corresponding to an areal loading of 15 mg/cm². Their high electrical conductivity allows the high volumetric capacity to be retained, even at high thickness, resulting in state-of-the-art areal capacities of >4.0 mAh/cm². Such values are competitive with their lithium-ion counterparts

1H NMR spectra of a typical cell extract.

<p>1H NMR spectra of a typical cell extract.</p

<i>S. cerevisiae</i> mitochondrial membrane potential (DYmt).

<p>A–G, S. cerevisiae treated with 0.13 mg/mL of GSE for 0, 0.5, 1, 1.5, 2, 2.5, and 3 h.</p

DAPI- and TUNEL-stained <i>S. cerevisiae</i> nuclei (10×60).

<p>A, E, control group; B, C, D, S. cerevisiae treated with 0.13 mg/mL of GSE for 3 h; A–C yeast cells stained with DAPI; D and E yeast cells stained with TUNEL.</p

Representative silver-stained 2-D gel of total cellular extracts from yeast cells treated with GSE.

<p>The spots that were altered upon treatment are represented by numbers (1–26).</p

<i>S. cerevisiae</i> intracellular ROS.

<p>A–D, S. cerevisiae treated with 0.13 mg/mL of GSE for 0 h, 1 h, 2 h, and 3 h.</p

Mesoporogen-Free Synthesis of Hierarchically Structured Zeolites with Variable Si/Al Ratios via a Steam-Assisted Crystallization Process

In the absence of additional mesoporous template, hierarchically structured zeolites (HSZs) with variable Si/Al ratios (30–150) have been successfully synthesized via a newly developed steam-assisted crystallization process. The synthesized materials were characterized with powder X-ray diffraction, nitrogen sorption measurement, scanning electron microscopy, transmission electron microscopy, inductively coupled plasma optical emission spectrometry, solid-state nuclear magnetic resonance, and ammonia temperature-programmed desorption. All these results prove that the synthesized materials feature high crystallinity (microporous framework) and auxiliary mesoporous structure. In the model reactions of isopropylbenzene and 1,3,5-triisopropylbenzene cracking, compared to purely microporous ZSM-5 counterparts, here synthesized HSZs exhibited markedly enhanced catalytic performances resulting from their enlarged external surface area and shortened diffusion length in the microporous system