Leaf transcriptome analysis of a subtropical evergreen broadleaf plant, wild oil-tea camellia (Camellia oleifera), revealing candidate genes for cold acclimation

Single nucleotide polymorphism (SNP) positions in genes of Camellia oleifera. Genotypes of samples from Jinggang (JG01-04) and Lu (LS01-04) mountains are shown. (XLSX 8324 kb

patS Minigenes Inhibit Heterocyst Development of Anabaena sp. Strain PCC 7120

The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode PatS C-terminal 4 (GSGR) to 8 (CDERGSGR) oligopeptides. When expressed by P(petE), P(patS), or P(rbcL) promoters, patS5 to patS8 inhibited heterocyst formation but patS4 did not. In contrast to the full-length patS gene, P(hepA)-patS5 failed to restore a wild-type pattern in a patS null mutant, indicating that PatS-5 cannot function in cell-to-cell signaling if it is expressed in proheterocysts. To establish the location of the PatS receptor, PatS-5 was confined within the cytoplasm as a gfp-patS5 fusion. The green fluorescent protein GFP-PatS-5 fusion protein inhibited heterocyst formation. Similarly, full-length PatS with a C-terminal hexahistidine tag inhibited heterocyst formation. These data indicate that the PatS receptor is located in the cytoplasm, which is consistent with recently published data indicating that HetR is a PatS target. We speculated that overexpression of other Anabaena strain PCC 7120 RGSGR-encoding genes might show heterocyst inhibition activity. In addition to patS and hetN, open reading frame (ORF) all3290 and an unannotated ORF, orf77, encode an RGSGR motif. Overexpression of all3290 and orf77 under the control of the petE promoter inhibited heterocyst formation, indicating that the RGSGR motif can inhibit heterocyst development in a variety of contexts

An ECG Monitoring and Alarming System Based On Android Smart Phone

ECG monitoring in daily life is an important means of treating heart disease. To make it easier for the medical to monitor the ECG of their patients outside the hospital, we designed and developed an ECG monitoring and alarming system based on Android smart phone. In our system, an ECG device collec...武汉大学、中国传媒大学、广东工业大学、中国计量学院、Engineering Information Institute、Scientific Research

Ascorbate antagonizes nickel ion to regulate JMJD1A expression in kidney cancer cells

Abnormal expression of histone demethylase Jumonji domain-containing protein 1A (JMJD1A) is associated with many kinds of cancers. JMJD1A is also a hypoxic response gene and its expression is regulated by hypoxia-inducible factor-1 (HIF-1). In this study, we determined the role of JMJD1A in development and hypoxia pathway. We also measured the expression of JMJD1A and two hypoxia factors glucose transporter 1 (GLUT1) and vascular endothelial growth factor (VEGF) in 786-0 and HEK293 cells treated with different concentrations of NiCl2 (2.5100 M) for 24 h, and found that JMJD1A mRNA and protein were up-regulated with increased concentrations of NiCl2. We then observed that ascorbate could retard the up-regulated effect of NiCl2-induced JMJD1A expression in a dose-dependent manner through decreasing the stability of HIF-1 protein. Immunohistochemical analysis further demonstrated ascorbate antagonized Ni-2-induced up-regulation of JMJD1A expression in 786-0, HEK293, and OS-RC-2 cells. These findings suggest that both Ni-2 and ascorbate can regulate the expression of histone demethylase JMJD1A, which is important for cancer development or inhibition

Improved YOLOv7 Algorithm for Small Object Detection in Unmanned Aerial Vehicle Image Scenarios

Object detection in unmanned aerial vehicle (UAV) images has become a popular research topic in recent years. However, UAV images are captured from high altitudes with a large proportion of small objects and dense object regions, posing a significant challenge to small object detection. To solve this issue, we propose an efficient YOLOv7-UAV algorithm in which a low-level prediction head (P2) is added to detect small objects from the shallow feature map, and a deep-level prediction head (P5) is removed to reduce the effect of excessive down-sampling. Furthermore, we modify the bidirectional feature pyramid network (BiFPN) structure with a weighted cross-level connection to enhance the fusion effectiveness of multi-scale feature maps in UAV images. To mitigate the mismatch between the prediction box and ground-truth box, the SCYLLA-IoU (SIoU) function is employed in the regression loss to accelerate the training convergence process. Moreover, the proposed YOLOv7-UAV algorithm has been quantified and compiled in the Vitis-AI development environment and validated in terms of power consumption and hardware resources on the FPGA platform. The experiments show that the resource consumption of YOLOv7-UAV is reduced by 28%, the mAP is improved by 3.9% compared to YOLOv7, and the FPGA implementation improves the energy efficiency by 12 times compared to the GPU

Effect of Unmanned Aerial Vehicle Flight Height on Droplet Distribution, Drift and Control of Cotton Aphids and Spider Mites

Unmanned aerial vehicles (UAVs), as emerging plant protection machinery, have the advantages of high operational efficiency, high speed, and low drift. The current study aimed to elucidate the characteristics of droplet distribution and drift, control efficiency on cotton aphids and spider mites, and attachment and absorption of cotton leaves during UAV spraying. Kromekote card and filter paper are used as samplers to collect droplets, and the droplet density, coverage rate, deposition, and drift percentage are statistically analyzed. The pooled results showed that the droplet uniformity, the droplet coverage rate, the deposition, and the drifting ability are higher when the UAV flight height was 2 m. The control effects by UAV spraying on cotton aphids and spider mites were 63.7% and 61.3%, respectively. These values are slightly inferior to those obtained through boom spraying. Cotton leaf attachment and absorption of spirodiclofen after UAV spraying were slightly lower than those after boom spraying, therefore, the control efficiency of cotton pests is slightly different. According to the different flight height operations by the UAV sprayer, the drift capability of the droplets at 2 m flight height was large, and the droplet uniformity and deposition were satisfactory. The research results could provide the theoretical basis and technical support for UAV operation

Study on the Mechanical and Leaching Characteristics of Permeable Reactive Barrier Waste Solidified by Cement-Based Materials

The durability against wet-dry (w-d) cycles is an important parameter for the service life design of solidified permeable reactive barrier (PRB) waste. This study introduces the potential use of cement, fly ash, and carbide slag (CFC) for the stabilization/solidification (S/S) of PRB waste. In this study, solidified PRB waste was subjected to different w-d cycles ranging in times from 0 to 10. By analyzing the mass loss, the unconfined compressive strength (UCS), initial resistivity (IR), and the Mn2+ leaching concentration under different durability conditions, the results demonstrate that these variables increased and then tended to decrease with the number of w-d cycles. The UCS of contaminated soil is significantly correlated with IR. Moreover, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD) analyses indicate that the hydration products calcium silicate hydrate (C-S-H) and ettringite (AFt) are the main reasons for the enhancement of the UCS. However, the increase in Mn2+ concentration leads to a decrease in hydration products and the compactness of solidified soil, which has negative effects for the UCS and the leaching ion concentration. In general, the durability exhibited by the PRB waste treated with S/S in this paper was satisfactory. This study can provide theoretical guidance for practical engineering applications