Additional file 2: of Physical interaction of STAT1 isoforms with TGF-β receptors leads to functional crosstalk between two signaling pathways in epithelial ovarian cancer

Figure S1. STAT1 expression in human epithelial-type ovarian tumors. Tissue microarray shows the immunohistochemical (IHC) staining of pSTAT1-Y701, pSTAT1-S727, and total STAT1 in serous, mucinous, endometrioid, transitional cell, and metastatic tumors. Figure S2. STAT1 expression in ovarian surface epithelial cells. a STAT1 mRNA expression detected by quantitative RT-PCR. b STAT1 protein expression detected by immunoblotting. c Densitometric analysis of the gels. Figure S3. Effect of TGF-β1 on the phosphorylation of STAT1. (DOCX 1304 kb

Additional file 1: of Physical interaction of STAT1 isoforms with TGF-β receptors leads to functional crosstalk between two signaling pathways in epithelial ovarian cancer

Table S1. PCR primer and siRNA sequence used in experiments. Table S2. Comparison of pSTAT1-Y701, pSTAT1-S727, and STAT1 immunostaining in the ovarian tissues. Table S3. The expression of pSTAT1-Y701, pSTAT1-S727, and STAT1 in human ovarian tissues. (DOCX 50 kb

Phylogenetic analyses of the six Inner Mongolia CVB4 strains and reference strains from GenBank using the 852-bp <i>VP1</i> region sequence.

<p>The strains indicated by blue squares are the CVB4 strains isolated in this study; the strain indicated by a red circle is the prototype CVB4 strain. The arrows indicate the strains in which whole genome sequencing was performed.</p

PCR and sequencing primers.

<p>PCR and sequencing primers.</p

Pairwise nucleotide and amino acid sequence identities between the CVB4 strains and prototype EV-B strains.

<p>Pairwise nucleotide and amino acid sequence identities between the CVB4 strains and prototype EV-B strains.</p

Similarity plot and bootscanning analyses of the whole genome of the Inner Mongolia CVB4 strains.

<p>Gene structure organization (a), similarity plot (b), and bootscanning analysis (c) of complete EV-B genomes using a sliding window of 200 nt moving in 20-nt steps. The HHHT34T/NM/CHN/2010 isolate was used as a query sequence and is indicated in the lower right corner. For each bootscanning analysis, the names of the viruses used as the query sequence are indicated in the upper right corner.</p