Prognostic value of CA125 in diffuse large B-cell lymphoma

BackgroundCarbohydrate antigen 125 (CA125) is one of the most commonly used tumor biomarker for evaluating the prognosis of solid neoplasms. It has been reported that serum CA125 is correlated with the prognosis of non-Hodgkin’s lymphoma. The objective of this study is to explore the clinical value of CA125 in diffuse large B-cell lymphoma (DLBCL).MethodsWe retrospectively analyzed the clinical and pathological data in a cohort of 315 newly diagnosed patients with DLBCL. In our case, the correlations between serum CA125 and clinicopathological parameters were analyzed. Kaplan-Meier survival curve and cox proportional hazards model were applied to evaluate the prognosis. The expression of CA125 in DLBCL paraffin tissues was detected by immunohistochemistry.Results82 patients (26%) with DLBCL had elevated serum CA125 levels at diagnosis. Elevated serum CA125 levels were associated with poor performances status, greater than or equal to 2 Extra-nodal sites, advanced Ann Arbor stage (III-IV), presence of B symptoms, presence of bulky mass, presence of effusion, intermediate/high-risk International Prognostic Index (IPI), elevated lactate dehydrogenase levels and reduced albumin levels. Patients with elevated serum CA125 levels at diagnosis had shorter progression free survival (PFS) and overall survival (OS). Multivariate analysis revealed that serum CA125, cell of origin, IPI score and albumin were independent prognostic factors for OS and PFS. In addition, the results of the immunohistochemistry indicated that none of the 82 DLBCL paraffin tissues expressed CA125 in lymphoma cells and the surrounding microenvironment cells.ConclusionsSerum CA125 detected at the initial diagnosis is a strong predictor of prognosis in patients with DLBCL

Evaluation of Bletilla striata Polysaccharide Deproteinized System Based on Entropy Weighted TOPSIS Model

The entropy TOPSIS model was used to compare the effects of Sevage method， acetonitrile method and trichloroacetic acid (TCA) method for removaling crude Bletilla striata polysaccharide (BSP) protein, and to explore the rationality of entropy TOPSIS for BSP deproteinization system evaluation. Based on the comprehensive score of BSP retention rate and protein removal rate, the optimal treatment conditions were screened out. Nine evaluation indicators including monosaccharide components, oxidative radical scavenging ability (ORAC), and half scavenging concentration of DPPH radicals (IC50) were constructed. Supplemented by UV and FTIR, the entropy TOPSIS was used to evaluate the results of three BSP deproteinization programs. After comprehensive score, the best extraction times of sevage method was 1 time. At same time, the protein removal rate was 22.9%, and the polysaccharide retention rate was 99.11%. The optimal mass concentration of the TCA method was 10%, when the protein removal rate was 70.64%, and the polysaccharide retention rate was 70.03%. Compared with the ORAC values and IC50 of the three polysaccharide, it was found that the value of polysaccharide ORAC treated by the acetonitrile method was higher than that of the positive control group (P<0.05), and the polysaccharides treated by the Sevage method had the strongest antioxidant activity. The BSP deproteinization evaluation system was analyzed by the entropy TOPSIS model, and the sevage method deproteinization effect was the best and the expected result. The results showed that the entropy TOPSIS model could be used in the evaluation of BSP deproteinization system

Expression and Prognostic Significance of PD-L2 in Diffuse Large B-Cell Lymphoma

Recent studies suggest that programmed death ligand-2 (PD-L2) constitutes an important antitumor immune response. Here, we investigated the relationship between PD-L2 expression and clinicopathological features in diffuse large B-cell lymphoma (DLBCL). Immunohistochemistry showed that positive expression of PD-L2 was observed in 45 of 181 newly diagnosed patients, including 14 cases with expression exclusively on tumor cells (TCs) and 31 cases with the expression on both TCs and immune cells (ICs) in the tumor microenvironment (TME). In 21 recurrent patients, positive expression of PD-L2 was present in six cases, including two cases with expression exclusively on TCs, and four cases with the expression on both TCs and ICs in the TME. Patients with PD-L2 tumor proportion score (TPS) ≥1% exhibited a better ECOG performance status (PS) (ECOG PS score &lt;2, P = 0.041), lower international prognostic index (IPI) score (P &lt; 0.001), and early Ann Arbor stage (Ann Arbor stage I or II, P = 0.010). Similarly, patients with PD-L2 immune proportion score (IPS) ≥1% also exhibited a better ECOG PS (ECOG PS score &lt; 2, P = 0.006) and lower IPI score (P = 0.001). Survival analysis showed that patients with PD-L2 TPS ≥1% exhibited prolonged overall survival (OS) and progression-free survival (PFS). However, survival analysis showed no prognostic significance based on expression of PD-L2 on ICs in the TME. TC PD-L2 expression was significantly associated with OS (P = 0.041) and PFS (P = 0.001). In the multivariate analysis, TC PD-L2 expression was an independent prognostic risk factor for PFS (P = 0.013), but not for OS (P = 0.249). Furthermore, we found that higher TC and IC PD-L2 expression was associated with higher objective response rate (ORR). Moreover, we demonstrated that the expression level of PD-L2 was positively correlated with the expression status of M1 macrophage markers CD86. Our findings highlight PD-L2 as a promising therapeutic target in DLBCL

A Study of the Problem Solving Behavior in Youth with Mental Retardation : In Comparison to Children without Mental Retardation on the Transformation Task

The present study was performed to evaluate the food safety of TT51-1, a new type of genetically modified rice that expresses the Cry1Ab/Ac protein (Bt toxin) and is highly resistant to most lepidopteran pests. Sixteen male and 16 female cynomolgus monkeys were randomly divided into four groups: conventional rice (non-genetically modified rice, non-GM rice), positive control, 17.5% genetically modified rice (GM rice) and 70% GM rice. Monkeys in the non-GM rice, positive control, and GM rice groups were fed on diets containing 70% non-GM rice, 17.5% GM rice or 70% GM rice, respectively, for 182 days, whereas animals in the positive group were intravenously injected with cyclophosphamide every other day for a total of four injections before the last treatment. Six months of treatment did not yield abnormal observations. Specifically, the following parameters did not significantly differ between the non-GM rice group and GM rice groups: body weight, food consumption, electrocardiogram, hematology, immuno-phenotyping of lymphocytes in the peripheral blood, mitogen-induced peripheral blood lymphocyte proliferation, splenocyte proliferation, KLH-T cell-dependent antibody response, organ weights and ratios, and histological appearance (p>0.05). Animals from the GM rice group differed from animals in the non-GM rice group (p<0.05) in several parameters: specifically, their body temperatures and serum alanine aminotransferase (ALT) levels were higher, whereas their levels of serum K+, Cl- and cytokines (IL-2, IL-4 and IL-5) were lower. Because dose- or time-dependent changes were not observed in this study and animals appeared histologically normal, the aforementioned differences were not considered to be adverse or related to the treatment with GM rice. In conclusion, a 6-month feeding study of TT51-1 did not show adverse immunotoxicological effects on cynomolgus monkeys

Texts of Kolima dialect of Yukaghir

<p>Clinical chemistry data of monkeys fed on diets containing GM rice or non-GM rice.</p

Estimating Area Coverage and Volume of Hot Lava by Integrating Multiple Satellite Remote Sensing Techniques

&amp;lt;p&amp;gt;Monitoring the regions that are prone to natural hazards is essential in disaster management, since early warnings can be issued. Airborne and space-borne remote sensing techniques are cost-effective in accomplishing the task. Estimating the area and volume of erupted lava can help researchers understand the volcanic processes and impact on land use and land cover. In this study, we developed a new algorithm to estimate areal coverage and volume of exposed hot lava by integrating the space-borne Interferometric Synthetic Aperture Radar (InSAR), thermal infrared, and Normalized Vegetation Distribution Index (NDVI) techniques. We applied this algorithm to the eruption of the East Rift Zone (ERZ) of the Kilauea volcano took place between May and August 2018 and estimated the areal coverage and volume of lava erupted. We compared the results of InSAR to those derived from airborne Light Detection and Ranging (LiDAR), and found that although air-borne LiDAR provides data with higher resolution and accuracy, InSAR is almost as good as LiDAR in monitoring deformed areas and has larger spatial and temporal coverage.&amp;lt;/p&amp;gt;</jats:p