128 research outputs found

    Histological observation of a gelatin sponge transplant loaded with bone marrow-derived mesenchymal stem cells combined with platelet-rich plasma in repairing an annulus defect - Fig 2

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    <p>The exposure of annulus fibrosus after decompression of the lamina, (A) sham group, arrow showing annulus fibrosus. (B)injury group,arrow showing a 1 × 1 cm defect of annulus fibrosus. (C)therapeutic group,arrow showing complexes including BMSCs, PRP,and Gelatin sponges.</p

    Quantitative analysis of type II collagen staining for each group (x ± s, n = 90).

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    <p>Quantitative analysis of type II collagen staining for each group (x ± s, n = 90).</p

    HE staining score for each group (x ± s, n = 90).

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    <p>HE staining score for each group (x ± s, n = 90).</p

    AB-PAS staining in each group after 3,6,12 weeks.

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    <p>(A-C) AB-PAS staining in the sharm group after 3,6,12 weeks (×100). (D-F) AB-PAS staining in the injury group after 3,6,12 weeks (×100). (G-I) AB-PAS staining in the therapeutic groupafter 3,6,12 weeks (×100). Black arrows:cartilage cells.</p

    Masson staining in each group after 3,6,12 weeks.

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    <p>(A-C) Masson staining in the sharm group after 3,6,12 weeks (×100). (D-F) Masson staining in the injury group after 3,6,12 weeks (×100). (G-I) Masson staining in the therapeutic group after 3,6,12 weeks (×100). Blue arrows:mature bone trabecular.Black arrows:muscle fiber connective tissue.</p

    Results of HE staining in each group after 3,6,12 weeks.

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    <p>(A-C) HE staining in the sharm group after 3,6,12 weeks (×100). (D-F) HE staining in the injury group after 3,6,12 weeks (×100). (G-I) HE staining in the therapeutic groupafter 3,6,12 weeks (×100).Blue arrows:collagen and matrix.Black arrows:cartilage cells.</p

    Masson staining score for each group (x ± s, n = 90).

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    <p>Masson staining score for each group (x ± s, n = 90).</p

    TEM results of treated tumors.

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    <p>Stimulated by heated (60°C) lipiodol perfusion, TEM results revealed that the tumor endothelial cell microvilli decreased (A, 5000×), the vascular endothelial cell endoplasmic reticulum expanded, and the chondriosome was swollen (B, 10000×).</p

    Expression of PCNA protein.

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    <p>As detected through immunohistochemistry, PCNA protein expression was detected mainly in viable VX2 tumor cells (brown; A, control 400<b>×</b>; B, treated 400<b>×</b>).</p
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